{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T15:59:23Z","timestamp":1775231963774,"version":"3.50.1"},"reference-count":15,"publisher":"The Company of Biologists","issue":"3","license":[{"start":{"date-parts":[[1990,11,1]],"date-time":"1990-11-01T00:00:00Z","timestamp":657417600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1990,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Mutant iv\/iv mice develop as if they have no sense of left and right, so the development of asymmetry is random: half normal, half as a mirror-image of normal, situs inversus. We have made aggregation chimeras of 8-cell stage iv\/iv and +\/+ embryos, transferred them into pseudopregnant mice, and examined their phenotype on day 10 of gestation. The contribution of mutant and wild-type cells to tissues of the embryo was estimated by strain-specific isozyme (GPI-1) analysis. We have also performed reciprocal embryo transfers, iv\/iv blastocysts into +\/+ mice, and vice versa. These transfers show that the development of handed asymmetry is determined by embryonic genotype, and is unaffected by the maternal environment (at least after day 3), or by the procedures of embryo collection, culture and transfer. Our observations on the development of 21 viable chimeric embryos show that neither iv\/iv nor +\/+ cells are dominant. All embryos (12) with less than 50 % contribution of iv\/iv cells to the heart developed with normal situs. Of 9 embryos with greater than 50 % iv\/iv cells, only 2 developed with inverted situs. These findings suggests that there was partial \u2018rescue\u2019 of embryos by some influence of normal over mutant cells. However, we cannot, statistically, exclude an alternative interpretation that cells are behaving autonomously. Interestingly, the embryos that developed with inverted situs were unique in having greater than two thirds contribution of iv\/iv cells to both the heart and the visceral yolk-sac.<\/jats:p>","DOI":"10.1242\/dev.110.3.949","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T01:38:47Z","timestamp":1619401127000},"page":"949-954","source":"Crossref","is-referenced-by-count":16,"title":["The development of handed asymmetry in aggregation chimeras of <i>situs inversus<\/i> mutant and wild-type mouse embryo"],"prefix":"10.1242","volume":"110","author":[{"given":"Nigel A.","family":"Brown","sequence":"first","affiliation":[{"name":"l MRC Experimental Embryology and Teratology Unit, Saint George\u2019s Hospital Medical School, Cranmer Terrace, London, SW17 ORE, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Afshan","family":"Mccarthy","sequence":"additional","affiliation":[{"name":"University College and Middlesex Hospital School of Medicine 2 Department of Anatomy and Developmental Biology , , Windeyer Building, Cleveland Street, London, W1P 6DB, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lewis","family":"Wolpert","sequence":"additional","affiliation":[{"name":"University College and Middlesex Hospital School of Medicine 2 Department of Anatomy and Developmental Biology , , Windeyer Building, Cleveland Street, London, W1P 6DB, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1990,11,1]]},"reference":[{"key":"2024071007213085700_DEVELOP_110_3_949C1","doi-asserted-by":"crossref","first-page":"637","DOI":"10.1242\/dev.107.3.637","article-title":"The development of asymmetry: the sidedness of drug-induced limb abnormalities is reversed in situs inversus mice","volume":"107","author":"Brown","year":"1989","journal-title":"Development"},{"key":"2024071007213085700_DEVELOP_110_3_949C2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1242\/dev.109.1.1","article-title":"The development of handedness in left\/right asymmetry","volume":"109","author":"Brown","year":"1990","journal-title":"Development"},{"key":"2024071007213085700_DEVELOP_110_3_949C3","doi-asserted-by":"crossref","first-page":"416","DOI":"10.1016\/0012-1606(84)90156-8","article-title":"Mouse trisomy 16 as an animal model of human trisomy 21 (Down\u2019s syndrome): formation of viable trisomy 16 diploid mouse embryos","volume":"101","author":"Cox","year":"1984","journal-title":"Devl Biol"},{"key":"2024071007213085700_DEVELOP_110_3_949C4","doi-asserted-by":"crossref","first-page":"181","DOI":"10.1002\/jez.1401830205","article-title":"Use of chimeras to transmit lethal genes in the mouse and to demonstrate allelism of the two X-linked genes jp and msd","volume":"183","author":"Eicher","year":"1973","journal-title":"J. exp. Zool"},{"key":"2024071007213085700_DEVELOP_110_3_949C5","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1038\/273149a0","article-title":"Activation of mammalian oocytes by intracellular injection of calcium","volume":"273","author":"Fulton","year":"1978","journal-title":"Nature"},{"key":"2024071007213085700_DEVELOP_110_3_949C6","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1093\/oxfordjournals.jhered.a106870","article-title":"Visceral inversion and associated anomalies in the mouse","volume":"50","author":"Hummel","year":"1959","journal-title":"J. Hered"},{"key":"2024071007213085700_DEVELOP_110_3_949C7","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1016\/0014-4835(76)90206-2","article-title":"Role of the pigment epithelium in inherited retinal degeneration analyzed with experimental mouse chimeras","volume":"23","author":"La Vail","year":"1976","journal-title":"Expl Eye Res"},{"key":"2024071007213085700_DEVELOP_110_3_949C8","doi-asserted-by":"crossref","first-page":"336","DOI":"10.1093\/oxfordjournals.jhered.a108749","article-title":"Random determination of a developmental process: reversal of normal visceral asymmetry in the mouse","volume":"67","author":"Layton","year":"1976","journal-title":"J. Hered"},{"key":"2024071007213085700_DEVELOP_110_3_949C9","first-page":"432","article-title":"Formation of genotypically mosaic mouse embryos","volume":"2","author":"Mintz","year":"1962","journal-title":"Am. Zool"},{"key":"2024071007213085700_DEVELOP_110_3_949C10","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1002\/jez.1401570210","article-title":"Formation of genetically mosaic mouse embryos, and early development of \u2018lethal\u2019 (t12\/t12) normal mosaics","volume":"157","author":"Mintz","year":"1964","journal-title":"J. exp. Zool"},{"key":"2024071007213085700_DEVELOP_110_3_949C11","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1242\/dev.99.4.473","article-title":"Rescue of developmental lens abnormalities in chimaeras of noncataractous and congenital cataractous mice","volume":"99","author":"Muggleton-Harris","year":"1987","journal-title":"Development"},{"key":"2024071007213085700_DEVELOP_110_3_949C12","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1083\/jcb.66.2.263","article-title":"Ultrastructural localisation of lectin binding sites on zonae pellucidae and plasma membranes of mammalian eggs","volume":"66","author":"Nicholson","year":"1975","journal-title":"J. Cell Biol"},{"key":"2024071007213085700_DEVELOP_110_3_949C13","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0002-9149(70)80002-9","article-title":"Mechanism of dextral looping of the embryonic heart","volume":"25","author":"Stalsberg","year":"1970","journal-title":"Am. J. Cardiol"},{"key":"2024071007213085700_DEVELOP_110_3_949C14","doi-asserted-by":"crossref","first-page":"857","DOI":"10.1038\/190857a0","article-title":"Mouse chimers developed from fused eggs","volume":"190","author":"Tarkowski","year":"1961","journal-title":"Nature"},{"key":"2024071007213085700_DEVELOP_110_3_949C15","first-page":"7","article-title":"Culture of mouse ova","volume":"14","author":"Whittingham","year":"1971","journal-title":"J. Reprod. 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