{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T20:47:18Z","timestamp":1789850838178,"version":"4.0.1"},"reference-count":40,"publisher":"The Company of Biologists","issue":"1","license":[{"start":{"date-parts":[[1991,5,1]],"date-time":"1991-05-01T00:00:00Z","timestamp":673056000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1991,5,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Using a monoclonal antibody that recognizes specifically a high polysialylated form of N-CAM (high PSA N-CAM), the temporal and spatial expression of this molecule was studied in developing spinal cord and neural crest derivatives of mouse truncal region. Temporal expression was analyzed on immunoblots of spinal cord and dorsal root ganglia (DRGs) extracts microdissected at different developmental stages. Analysis of the ratio of high PSA N-CAM to total N-CAM indicated that sialylation and desialylation are independently regulated from the expression of polypeptide chains of N-CAM. Motoneurons, dorsal root ganglia cells and commissural neurons present a homogeneous distribution of high PSA N-CAMs on both their cell bodies and their neurites. Sialylation of N-CAM can occur in neurons after their aggregation in peripheral ganglia as demonstrated for dorsal root ganglia at E12. Furthermore, peripheral ganglia express different levels of high PSA N-CAM. With in vitro models using mouse neural crest cells, we found that expression of high PSA N-CAM was restricted to cells presenting an early neuronal phenotype, suggesting a common regulation for the expression of high PSA N-CAM molecules, neurofilament proteins and sodium channels. Using perturbation experiments with endoneuraminidase, we confirmed that high PSA N-CAM molecules are involved in fasciculation and neuritic growth when neurons derived from neural crest grow on collagen substrata. However, we demonstrated that these two parameters do not appear to depend on high PSA N-CAM molecules when cells were grown on a fibronectin substratum, indicating the existence of a hierarchy among adhesion molecules.<\/jats:p>","DOI":"10.1242\/dev.112.1.69","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T01:42:05Z","timestamp":1619401325000},"page":"69-82","source":"Crossref","is-referenced-by-count":50,"title":["Analysis of high PSA N-CAM expression during mammalian spinal cord and peripheral nervous system development"],"prefix":"10.1242","volume":"112","author":[{"given":"Sylvie","family":"Boisseau","sequence":"first","affiliation":[{"name":"1 Laboratoire de Neurobiologie Cellulaire et Mol\u00e9culaire du CNRS, 91198 Gif sur Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00ebl","family":"Nedelec","sequence":"additional","affiliation":[{"name":"2 URA 179 CNRS, \u2018Biologie de la diff\u00e9renciation cellulaire, Facult\u00e9 des Sciences de Lummy, Case 901, 13288 Marseille Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"V\u00e9ronique","family":"Poirier","sequence":"additional","affiliation":[{"name":"1 Laboratoire de Neurobiologie Cellulaire et Mol\u00e9culaire du CNRS, 91198 Gif sur Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genevi\u00e8ve","family":"Rougon","sequence":"additional","affiliation":[{"name":"2 URA 179 CNRS, \u2018Biologie de la diff\u00e9renciation cellulaire, Facult\u00e9 des Sciences de Lummy, Case 901, 13288 Marseille Cedex, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Simonneau","sequence":"additional","affiliation":[{"name":"1 Laboratoire de Neurobiologie Cellulaire et Mol\u00e9culaire du CNRS, 91198 Gif sur Yvette, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[1991,5,1]]},"reference":[{"key":"2023050312232745500_DEVELOP_112_1_69C1","doi-asserted-by":"publisher","first-page":"286","DOI":"10.1016\/0012-1606(82)90035-5","article-title":"Localization of vimentin, the nonspecific intermediate filament protein, in embryonal glia and in early differentiating neurons In vivo and in vitro immunofluorescence study of the rat embryo with vimentin and neurofilament antisera","volume":"91","author":"Bignami","year":"1982","journal-title":"Devi Biol"},{"key":"2023050312232745500_DEVELOP_112_1_69C2","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1242\/dev.106.4.665","article-title":"Mammalian neuronal differentiation early expression of a neuronal phenotype from mouse neural crest cells in a chemically defined culture 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