{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T17:15:20Z","timestamp":1778865320685,"version":"3.51.4"},"reference-count":25,"publisher":"The Company of Biologists","issue":"2","license":[{"start":{"date-parts":[[1991,6,1]],"date-time":"1991-06-01T00:00:00Z","timestamp":675734400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1991,6,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Mouse cerebellar granule cells showed two types of migration behavior in microexplant cultures. They first migrated along their neurites, showing the typical contact guidance, and then oriented themselves at right angles to the parallel neurites, thus exhibiting the \u2018perpendicular contact guidance\u2019 (Nakatsuji, N. and Nagata, I. 1989 Development, 106, 441\u2013447).<\/jats:p>\n               <jats:p>To study whether other neurons have the capacity to show similar \u2018perpendicular contact guidance\u2019, we cultured dissociated neuroblasts from various parts of CNS or PNS on parallel neurite bundles. The PNS neuroblasts always extended their processes parallel to the neurite bundle. In contrast, almost all kinds of CNS neuroblasts tested oriented their processes both perpendicular and parallel to the neurite bundles that were all free of glia. Time-lapse video recording revealed that neuroblasts migrated in both directions. Thus, CNS neuroblasts possess the capacity to migrate and extend their processes at right angles to the substratum of heterotypic neurite bundles, which may play an important role in histogenesis of the CNS during development.<\/jats:p>","DOI":"10.1242\/dev.112.2.581","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T01:55:54Z","timestamp":1619402154000},"page":"581-590","source":"Crossref","is-referenced-by-count":34,"title":["Rodent CNS neuroblasts exhibit both perpendicular and parallel contact guidance on the aligned parallel neurite bundle"],"prefix":"10.1242","volume":"112","author":[{"given":"Isao","family":"Nagata","sequence":"first","affiliation":[{"name":"Department of Genetics 1 , Tokyo Metropolitan Institute for Neurosciences, 2\u20136 Musashidai, Fuchu, Tokyo 183, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norio","family":"Nakatsuji","sequence":"additional","affiliation":[{"name":"Division of Developmental Biology 2 , Meiji Institute of Health Science, 540 Naruda, Odawara 250, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1991,6,1]]},"reference":[{"key":"2023050320421074300_DEVELOP_112_2_581C1","doi-asserted-by":"publisher","first-page":"353","DOI":"10.1002\/cne.901450305","article-title":"Postnatal development of the cerebellar cortex in the rat. I. The external germinal layer and the transitional molecular layer","volume":"145","author":"Altman","year":"1972","journal-title":"J. comp. Neurol."},{"key":"2023050320421074300_DEVELOP_112_2_581C2","doi-asserted-by":"publisher","first-page":"36","DOI":"10.1016\/0014-4886(90)90061-V","article-title":"Horizontal compartmentation in the germinal matrices and intermediate zone of the embryonic rat cerebral cortex","volume":"107","author":"Altman","year":"1990","journal-title":"Expl Neurol."},{"key":"2023050320421074300_DEVELOP_112_2_581C3","doi-asserted-by":"publisher","first-page":"692","DOI":"10.1126\/science.3055291","article-title":"Axon guidance and the patterning of neuronal projections in vertebrates","volume":"242","author":"Dodd","year":"1988","journal-title":"Science"},{"key":"2023050320421074300_DEVELOP_112_2_581C4","doi-asserted-by":"publisher","first-page":"325","DOI":"10.1016\/0304-3940(82)90079-9","article-title":"Selection of mouse cerebellar cells in serum free, hormonally defined media: Survival of neurons","volume":"28","author":"Fischer","year":"1982","journal-title":"Neurosci. Lett."},{"key":"2023050320421074300_DEVELOP_112_2_581C5","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1083\/jcb.32.2.277","article-title":"Quantitative analysis of cell proliferation and differentiation in the cortex of the postnatal mouse cerebellum","volume":"32","author":"Fujita","year":"1967","journal-title":"J. Cell Biol."},{"key":"2023050320421074300_DEVELOP_112_2_581C6","doi-asserted-by":"publisher","first-page":"423","DOI":"10.1016\/0012-1606(87)90179-5","article-title":"Response of sensory neurites and growth cones to patterned substrata of laminin and fibronectin in vitro.","volume":"121","author":"Gundersen","year":"1987","journal-title":"Devi Biol."},{"key":"2023050320421074300_DEVELOP_112_2_581C7","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/0166-2236(90)90044-B","article-title":"Riding the glial monorail: a common mechanism for glial-guided neuronal migration in different regions of the developing mammalian brain","volume":"13","author":"Hatten","year":"1990","journal-title":"Trends Neurosci."},{"key":"2023050320421074300_DEVELOP_112_2_581C8","doi-asserted-by":"publisher","first-page":"1113","DOI":"10.1016\/0896-6273(89)90178-5","article-title":"Small inhibitory cerebellar interneurons grow in a perpendicular orientation to granule cell neurites in culture","volume":"2","author":"Hekmat","year":"1989","journal-title":"Neuron"},{"key":"2023050320421074300_DEVELOP_112_2_581C9","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1016\/0006-8993(88)91314-5","article-title":"Recognition of artificial microstructures by sensory nerve fibers in culture","volume":"446","author":"Hirono","year":"1988","journal-title":"Brain Res."},{"key":"2023050320421074300_DEVELOP_112_2_581C10","doi-asserted-by":"publisher","first-page":"867","DOI":"10.1523\/JNEUROSCI.06-03-00867.1986","article-title":"Migration of neuroblasts along preexisting axonal tracts during prenatal cerebellar development","volume":"6","author":"Hynes","year":"1986","journal-title":"J. Neurosci."},{"key":"2023050320421074300_DEVELOP_112_2_581C11","doi-asserted-by":"publisher","first-page":"1595","DOI":"10.1523\/JNEUROSCI.07-05-01595.1987","article-title":"The selective inhibition of growth cone extension by specific neurites in culture","volume":"7","author":"Kapfhammer","year":"1987","journal-title":"J. Neurosci."},{"key":"2023050320421074300_DEVELOP_112_2_581C12","doi-asserted-by":"publisher","first-page":"112","DOI":"10.1002\/cne.902580109","article-title":"Golgi studies on the development of granule cells of the rat olfactory bulb with reference to migration in the subependymal layer","volume":"258","author":"Kishi","year":"1987","journal-title":"J. comp. Neurol."},{"key":"2023050320421074300_DEVELOP_112_2_581C13","doi-asserted-by":"publisher","first-page":"4098","DOI":"10.1523\/JNEUROSCI.08-11-04098.1988","article-title":"Controlled outgrowth of dissociated neurons on patterned substrates","volume":"8","author":"Kleinfeld","year":"1988","journal-title":"J. Neurosci."},{"key":"2023050320421074300_DEVELOP_112_2_581C14","doi-asserted-by":"publisher","first-page":"92","DOI":"10.1016\/0012-1606(75)90379-6","article-title":"Cell-to-substratum adhesion and guidance of axonal elongation","volume":"44","author":"Letourneau","year":"1975","journal-title":"Devi Biol."},{"key":"2023050320421074300_DEVELOP_112_2_581C15","doi-asserted-by":"publisher","first-page":"127","DOI":"10.1016\/0014-4827(79)90263-5","article-title":"Cell-substratum adhesion of neurite growth cones, and its role in neurite elongation","volume":"124","author":"Letourneau","year":"1979","journal-title":"Expl Cell Res."},{"key":"2023050320421074300_DEVELOP_112_2_581C16","first-page":"911","article-title":"Either parallel or perpendicular - orientation pattern of dissociated single neuroblasts on neurite bundles of mouse CNS or PNS","volume":"14","author":"Nagata","year":"1989","journal-title":"Cell Struct, and Funci."},{"key":"2023050320421074300_DEVELOP_112_2_581C17","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1016\/0165-3806(86)90190-2","article-title":"Neuronal influence on antigenic marker profile, cell shape and proliferation of cultured astrocytes obtained by microdissection of distinct layers from the early postnatal mouse cerebellum","volume":"24","author":"Nagata","year":"1986","journal-title":"Dev. Brain Res."},{"key":"2023050320421074300_DEVELOP_112_2_581C18","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1016\/0165-3806(90)90222-K","article-title":"Granule cell behavior on laminin in mouse cerebellar microexplant cultures","volume":"52","author":"Nagata","year":"1990","journal-title":"Dev. Brain Res."},{"key":"2023050320421074300_DEVELOP_112_2_581C19","doi-asserted-by":"publisher","first-page":"441","DOI":"10.1242\/dev.106.3.441","article-title":"Paradoxical perpendicular contact guidance displayed by mouse cerebellar granule cell neurons in vitro.","volume":"106","author":"Nakatsuji","year":"1989","journal-title":"Development"},{"key":"2023050320421074300_DEVELOP_112_2_581C20","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1007\/978-3-642-65581-4_3","article-title":"Granule cells","volume-title":"Cerebellar Cortex Cytology and Organization,","author":"Palay","year":"1974"},{"key":"2023050320421074300_DEVELOP_112_2_581C21","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/0006-8993(73)90617-3","article-title":"Neuronal migration, with special reference to developing human brain: A review","volume":"62","author":"Sidman","year":"1973","journal-title":"Brain Res."},{"key":"2023050320421074300_DEVELOP_112_2_581C22","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1002\/cne.901890106","article-title":"A mechanism for the guidance and topographic patterning of retinal ganglion cell axons","volume":"189","author":"Silver","year":"1980","journal-title":"J. comp. 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Neurol."},{"key":"2023050320421074300_DEVELOP_112_2_581C25","doi-asserted-by":"publisher","first-page":"1342","DOI":"10.1126\/science.3137660","article-title":"Clonally related cortical cells show several migration patterns","volume":"241","author":"Walsh","year":"1988","journal-title":"Science"}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/112\/2\/581\/2822633\/develop_112_2_581.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/112\/2\/581\/2822633\/develop_112_2_581.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,3]],"date-time":"2023-05-03T20:42:53Z","timestamp":1683146573000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/112\/2\/581\/36863\/Rodent-CNS-neuroblasts-exhibit-both-perpendicular"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,6,1]]},"references-count":25,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1991,6,1]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.112.2.581","relation":{},"ISSN":["0950-1991","1477-9129"],"issn-type":[{"value":"0950-1991","type":"print"},{"value":"1477-9129","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,6,1]]}}}