{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,17]],"date-time":"2025-10-17T14:15:50Z","timestamp":1760710550085},"reference-count":52,"publisher":"The Company of Biologists","issue":"2","license":[{"start":{"date-parts":[[1991,6,1]],"date-time":"1991-06-01T00:00:00Z","timestamp":675734400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1991,6,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The extracellular matrix protein, tenascin, appears in a restricted pattern during organ morphogenesis. Tenascin accumulates along developing peripheral nerves as they leave the spinal cord and enter the limb mesenchyme (Wehrle and Chiquet, Development 110, 401\u2013 415, 1990). Here we found that most but not all tenascin deposited along growing nerves is of glial origin. By in situ hybridization with a tenascin cDNA probe, we determined the site of tenascin mRNA accumulation both in normal and nerve-free limbs. In normal wing buds, tenascin mRNA was first detected within the developing limb nerves. Vinculin-positive glial precursor cells, which comigrate with the axons, are the likely source of this tenascin message. In nerveless wing grafts, tenascin was first expressed in tendon primordia in the absence, and thus independently, from innervation. In contrast to normal limbs, grafted wing buds neither contained vinculin-positive glial precursor cells, nor expressed tenascin in regions proximal to tendon primordia. In normal wing buds, tenascin deposited by tendon primordia transiently parallels and surrounds certain developing nerves. After the major nerve pattern is established, tenascin mRNA disappears from nerves in the upper limb, but is expressed in perichondrium and tendons. We propose that glial tenascin facilitates the penetration of axons into the limb bud and is important for nerve fasciculation. In some places, early tendon primordia might help to guide the migration of axons and glial precursor cells towards their target. Abbreviations: ECM, extracellular matrix; TBS, Tris buffered saline; mAb, monoclonal antibody, IgG, immunoglobulin gamma; SDS, sodium dodecyl sulfate; TGF-\u03b2, transforming growth factor-\u03b2; FCS, fetal calf serum.<\/jats:p>","DOI":"10.1242\/dev.112.2.627","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T01:56:10Z","timestamp":1619402170000},"page":"627-637","source":"Crossref","is-referenced-by-count":41,"title":["Nerve-dependent and -independent tenascin expression in the developing chick limb bud"],"prefix":"10.1242","volume":"112","author":[{"given":"Bernhard","family":"Wehrle-Haller","sequence":"first","affiliation":[{"name":"Department of Biophysical Chemistry 1 , Biocenter of the University, Klingelbergstrasse 70, CH-4056 Basel, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manuel","family":"Koch","sequence":"additional","affiliation":[{"name":"Department of Biophysical Chemistry 1 , Biocenter of the University, Klingelbergstrasse 70, CH-4056 Basel, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Baumgartner","sequence":"additional","affiliation":[{"name":"Friedrich-Miescher-lnstitut 2 , Basel, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00fcrg","family":"Spring","sequence":"additional","affiliation":[{"name":"Friedrich-Miescher-lnstitut 2 , Basel, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matthias","family":"Chiquet","sequence":"additional","affiliation":[{"name":"Department of Biophysical Chemistry 1 , Biocenter of the University, Klingelbergstrasse 70, CH-4056 Basel, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1991,6,1]]},"reference":[{"key":"2023050320420664700_DEVELOP_112_2_627C1","doi-asserted-by":"publisher","first-page":"1247","DOI":"10.1101\/gad.1.10.1247","article-title":"Structure of two genes at the gooseberry locus related to the paired gene and their spatial expression during Drosophila embryogenesis","volume":"1","author":"Baumgartner","year":"1987","journal-title":"Genes Devi."},{"key":"2023050320420664700_DEVELOP_112_2_627C2","doi-asserted-by":"publisher","first-page":"1822","DOI":"10.1083\/jcb.99.5.1822","article-title":"Biologically active synthetic peptides as probes of embryonic development: a competitive inhibitor of fibronectin function inhibits gastrulation in amphibian embryos and neural crest cell migration in avian embryos","volume":"99","author":"Boucaut","year":"1984","journal-title":"J. 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