{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T17:05:36Z","timestamp":1791133536790,"version":"4.1.0"},"reference-count":48,"publisher":"The Company of Biologists","issue":"4","license":[{"start":{"date-parts":[[1991,12,1]],"date-time":"1991-12-01T00:00:00Z","timestamp":691545600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1991,12,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>By immunofluorescence on cytospin preparations and on semithin sections of mouse pancreatic buds, we have found glucagon and pancreatic polypeptide (PP)-containing cells at embryonal day 10.5 (E 10.5) in dorsal buds and at E 11.5 in ventral buds. Insulin-containing cells appear in dorsal buds at E 11.5, and one to two days later in ventral buds. Somatostatin-containing cells are detectable from E 13.5 in both dorsal and ventral buds. A quantitative analysis shows that up to E 15.5, PP-containing cells are relatively abundant in both buds. By PCR amplification of oligo(dT)-primed cDNAs prepared from total pancreatic RNA, we also detect PP mRNA from E 10.5 onwards, thus confirming the early expression of the PP gene in the developing mouse pancreas.<\/jats:p>\n               <jats:p>Analysis of endocrine cells in situ suggests three major patterns of cell distribution in embryonic pancreas. First, individual hormone-containing cells are located within the epithelium of pancreatic ducts. In both dorsal and ventral buds, the majority of these endocrine cells contain PP, but many also contain glucagon, insulin or somatostatin. Secondly, clusters of endocrine cells are found in the pancreatic interstitium. Many of these cells contain both glucagon and PP which, by inununogold labelling of consecutive thin sections, can be shown to co-exist within individual secretory granules. Finally, starting on E 18.5, typical islets are formed with centrally located B cells and with the adult \u2018one cell \u2013 one hormone\u2019 phenotype. These results suggest an intriguing ontog\u00e9nie relationship between A- and PP-cells, and also indicate that PP-containing cells may occupy a hitherto unexpected place in the lineage of endocrine islet cells.<\/jats:p>","DOI":"10.1242\/dev.113.4.1257","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T00:58:38Z","timestamp":1619398718000},"page":"1257-1265","source":"Crossref","is-referenced-by-count":215,"title":["Embryogenesis of the murine endocrine pancreas; early expression of pancreatic polypeptide gene"],"prefix":"10.1242","volume":"113","author":[{"given":"Pedro-Luis","family":"Herrera","sequence":"first","affiliation":[{"name":"University of Geneva Medical School Institute of Histology and Embryology , , CH-1211 Geneva 4, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joaquin","family":"Huarte","sequence":"additional","affiliation":[{"name":"University of Geneva Medical School Institute of Histology and Embryology , , CH-1211 Geneva 4, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Sanvito","sequence":"additional","affiliation":[{"name":"University of Geneva Medical School Institute of Histology and Embryology , , CH-1211 Geneva 4, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Meda","sequence":"additional","affiliation":[{"name":"University of Geneva Medical School Institute of Histology and Embryology , , CH-1211 Geneva 4, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lelio","family":"Orci","sequence":"additional","affiliation":[{"name":"University of Geneva Medical School Institute of Histology and Embryology , , CH-1211 Geneva 4, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Dominique","family":"Vassalli","sequence":"additional","affiliation":[{"name":"University of Geneva Medical School Institute of Histology and Embryology , , CH-1211 Geneva 4, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[1991,12,1]]},"reference":[{"key":"2023050315314603400_DEVELOP_113_4_1257C1","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1007\/BF00495439","article-title":"Peptide YY (PYY) immunoreactivity is co-stored with glucagonrelated immunoreactants in endocrine cells of the gut and pancreas","volume":"80","author":"Ali Rachedi","year":"1984","journal-title":"Histochemistry"},{"key":"2023050315314603400_DEVELOP_113_4_1257C2","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1016\/0092-8674(88)90391-1","article-title":"Hybrid insulin genes reveal a developmental lineage for pancreatic endocrine cells and imply a relationship with neurons","volume":"53","author":"Alpert","year":"1988","journal-title":"Cell"},{"key":"2023050315314603400_DEVELOP_113_4_1257C3","doi-asserted-by":"publisher","first-page":"1323","DOI":"10.1126\/science.390711","article-title":"Endocrine pancreas: three-dimensional reconstruction shows two types of islets of Langerhans","volume":"206","author":"Baetens","year":"1979","journal-title":"Science"},{"key":"2023050315314603400_DEVELOP_113_4_1257C4","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1177\/28.2.7354212","article-title":"Quantitative immunocytochemical localization of pancreatic secretory proteins in subcellular compartments of the rat acinar cell","volume":"28","author":"Bendayan","year":"1980","journal-title":"J. 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