{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T14:01:55Z","timestamp":1753884115904,"version":"3.41.2"},"reference-count":20,"publisher":"The Company of Biologists","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,1,1]]},"abstract":"<jats:p>Cell-based bone regeneration strategies offer promise for traumatic bone injuries, congenital defects, non-union fractures and other skeletal pathologies. Postnatal bone remodeling and fracture healing provide evidence that an osteochondroprogenitor cell is present in adult life that can differentiate to remodel or repair the fractured bone. However, cell-based skeletal repair in the clinic is still in its infancy, mostly due to poor characterization of progenitor cells and lack of knowledge about their in vivo behavior. Here, we took a combined approach of high-throughput screening, flow-based cell sorting and in vivo transplantation to isolate markers that identify osteochondroprogenitor cells. We show that the presence of tetraspanin CD9 enriches for osteochondroprogenitors within CD105+ mesenchymal cells and that these cells readily form bone upon transplantation. In addition, we have used Thy1.2 and the ectonucleotidase CD73 to identify subsets within the CD9+ population that lead to endochondral or intramembranous-like bone formation. Utilization of this unique cell surface phenotype to enrich for osteochondroprogenitor cells will allow for further characterization of the molecular mechanisms that regulate their osteogenic properties.<\/jats:p>","DOI":"10.1242\/dev.113571","type":"journal-article","created":{"date-parts":[[2015,1,7]],"date-time":"2015-01-07T11:44:45Z","timestamp":1420631085000},"source":"Crossref","is-referenced-by-count":6,"title":["Tetraspanin CD9 and ectonucleotidase CD73 identify an osteochondroprogenitor population with elevated osteogenic properties"],"prefix":"10.1242","author":[{"given":"Anju","family":"Singh","sequence":"first","affiliation":[{"name":"Department of Preclinical Innovation, National Center for Advancing Translational Sciences, National Institutes of Health, Rockville, MD 20850, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chantel","family":"Lester","sequence":"additional","affiliation":[{"name":"Division of Rheumatology, Brigham and Women's Hospital, Boston, MA 02115, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca","family":"Drapp","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dorothy Z.","family":"Hu","sequence":"additional","affiliation":[{"name":"Endocrine Unit, Massachusetts General Hospital, Boston, MA 02114, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurie H.","family":"Glimcher","sequence":"additional","affiliation":[{"name":"Department of Medicine, Weill Cornell Medical College, New York, NY 10065, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dallas","family":"Jones","sequence":"additional","affiliation":[{"name":"Abide Therapeutics, San Diego, CA 92121, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","reference":[{"key":"2021042608181040900_DEV113571C1","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1007\/978-1-61779-415-5_2","article-title":"Osteoblast isolation from murine calvaria and long bones","volume":"816","author":"Bakker","year":"2012","journal-title":"Methods Mol. 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