{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T11:56:13Z","timestamp":1791114973137,"version":"4.1.0"},"reference-count":28,"publisher":"The Company of Biologists","issue":"4","license":[{"start":{"date-parts":[[1993,8,1]],"date-time":"1993-08-01T00:00:00Z","timestamp":744163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1993,8,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The early progenitor cells to the pancreatic islets in the mouse have been characterized so as to re-examine their possible lineage relationships to the four islet cell types found in mature islets. Insulin and glucagon were both first expressed at embryonic day 9.5, and many cells co-expressed these two markers, as shown by light and electron microscopic analysis using double-label immunohistochemistry. Incubation of embryonic pancreas with 1% glutaraldehyde, a fixative commonly used by electron microscopists, abolished this reactivity, thereby explaining reported difficulties in detecting these precursor cells. Using antisera specific for neu-ropeptide Y (NPY) a peptide with considerable homology to pancreatic polypeptide (PP), we show that NPY first appears with insulin and glucagon immunore-activity at E9.5, and is co-expressed with glucagon in a majority of adult cells. As we have previously reported, PP itself is first detectable immunocytochemically at postnatal day 1 with PP-specific antibodies. However, antibodies raised against bovine PP are shown by dot blotting to recognize NPY with comparable avidity, indi-cating that a recent report of islet progenitor cells containing PP at E9.5 (Herrera, P. L., Huarte, J., Sanvito, F., Meda, P., Orci, L. and Vassalli, J. D. (1991) Devel-opment 113, 1257-1265), actually represents cross-reactivity to NPY. The data support a model in which early precursor cells to the endocrine pancreas co-activate and co-express a set of islet cell hormone and neural genes, whose expression is both selectively increased and extinguished as development proceeds, concomitant with a restriction to the patterns of expression characteristic of mature islet cell types.<\/jats:p>","DOI":"10.1242\/dev.118.4.1031","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T01:27:02Z","timestamp":1619400422000},"page":"1031-1039","source":"Crossref","is-referenced-by-count":301,"title":["Precursor cells of mouse endocrine pancreas coexpress insulin, glucagon and the neuronal proteins tyrosine hydroxylase and neuropeptide Y, but not pancreatic polypeptide"],"prefix":"10.1242","volume":"118","author":[{"given":"G.","family":"Teitelman","sequence":"first","affiliation":[{"name":"Department of Anatomy and Cell Biology 1 , SUNY Health Science Center, Brooklyn, NY 11203, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Alpert","sequence":"additional","affiliation":[{"name":"Department of Pathology 2 , Stanford Univ School of Medicine, Stanford, CA 94305, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. M.","family":"Polak","sequence":"additional","affiliation":[{"name":"Department of Histochemistry 3 , Royal Postgraduate Medical School, Hammersmith Hospital, Du Cane Road, London W12 ONN, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Martinez","sequence":"additional","affiliation":[{"name":"Department of Cytology and Histology 4 , Universidad de Navarra, 31080 Pamplona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Hanahan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Biophysics 5 , Hormone Research Institute, University of San Francisco, San Francisco, CA 94143-3612, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[1993,8,1]]},"reference":[{"key":"2024121723070778700_DEVELOP_118_4_1031C1","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1146\/annurev.iy.05.040187.001545","article-title":"Early events in T cell maturation","volume":"5","author":"Adkins","year":"1987","journal-title":"Ann Rev. 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