{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T16:38:44Z","timestamp":1791131924054,"version":"4.1.0"},"reference-count":45,"publisher":"The Company of Biologists","issue":"10","license":[{"start":{"date-parts":[[1995,10,1]],"date-time":"1995-10-01T00:00:00Z","timestamp":812505600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1995,10,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The adult Drosophila wing (as the other appendages) is subdivided into anterior and posterior compartments that exhibit characteristic patterns. The engrailed (en) gene has been proposed to be paramount in the specification of the posterior compartment identity. Here, we explore the adult en function by targeting its expression in different regions of the wing disc. In the anterior compartment, ectopic en expression gives rise to the substitution of anterior structures by posterior ones, thus demonstrating its role in specification of posterior patterns. The en-expressing cells in the anterior compartment also induce high levels of the hedgehog (hh) and decapentaplegic (dpp) gene products, which results in local duplications of anterior patterns.<\/jats:p>\n               <jats:p>Besides, hh is able to activate en and the engrailed-related gene invected (inv) in this compartment. In the posterior compartment we find that elevated levels of en product result in partial inactivation of the endogenous en and inv genes, indicating the existence of a negative autoregulatory mechanism. We propose that en has a dual role: a general one for patterning of the appendage, achieved through the activation of secreted proteins like hh and dpp, and a more specific one, determining posterior identity, in which the inv gene may be implicated.<\/jats:p>","DOI":"10.1242\/dev.121.10.3447","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T02:13:21Z","timestamp":1619403201000},"page":"3447-3456","source":"Crossref","is-referenced-by-count":142,"title":["The function of <i>engrailed<\/i> and the specification of <i>Drosophila<\/i> wing pattern"],"prefix":"10.1242","volume":"121","author":[{"given":"Isabel","family":"Guill\u00e9n","sequence":"first","affiliation":[{"name":"Universidad Aut\u00f3noma de Madrid Centro de Biolog\u00eda Molecular Severo Ochoa (CSIC-UAM) , , Cantoblanco, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose L.","family":"Mullor","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Madrid Centro de Biolog\u00eda Molecular Severo Ochoa (CSIC-UAM) , , Cantoblanco, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javier","family":"Capdevila","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Madrid Centro de Biolog\u00eda Molecular Severo Ochoa (CSIC-UAM) , , Cantoblanco, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernesto","family":"S\u00e1nchez-Herrero","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Madrid Centro de Biolog\u00eda Molecular Severo Ochoa (CSIC-UAM) , , Cantoblanco, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gin\u00e9s","family":"Morata","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Madrid Centro de Biolog\u00eda Molecular Severo Ochoa (CSIC-UAM) , , Cantoblanco, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Guerrero","sequence":"additional","affiliation":[{"name":"Universidad Aut\u00f3noma de Madrid Centro de Biolog\u00eda Molecular Severo Ochoa (CSIC-UAM) , , Cantoblanco, 28049 Madrid, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[1995,10,1]]},"reference":[{"key":"2025011515115675900_DEVELOP_121_10_3447C1","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1038\/368208a0","article-title":"Compartment boundaries and the control of Drosophila limb pattern by hedgehog protein","volume":"368","author":"Basler","year":"1994","journal-title":"Nature"},{"key":"2025011515115675900_DEVELOP_121_10_3447C2","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1242\/dev.111.3.657","article-title":"An extensive 3\u2032 cis-regulatory region directs the imaginal disc expression of decapentaplegic, a member of a TGF-\u03b2 family in Drosophila","volume":"111","author":"Blackman","year":"1991","journal-title":"Development"},{"key":"2025011515115675900_DEVELOP_121_10_3447C3","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1242\/dev.115.1.21","article-title":"engrailed expression in the anterior lineage compartment of the developing wing blade of Drosophila","volume":"115","author":"Blair","year":"1992","journal-title":"Development"},{"key":"2025011515115675900_DEVELOP_121_10_3447C4","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1242\/dev.118.2.401","article-title":"Targeted gene expression as a means of altering cell fates and generating dominant phenotypes","volume":"118","author":"Brand","year":"1993","journal-title":"Development"},{"key":"2025011515115675900_DEVELOP_121_10_3447C5","doi-asserted-by":"publisher","first-page":"2649","DOI":"10.1002\/j.1460-2075.1986.tb04547.x","article-title":"engrailed gene expression in Drosophila imaginal 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