{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T00:11:50Z","timestamp":1783555910809,"version":"3.55.0"},"reference-count":50,"publisher":"The Company of Biologists","issue":"11","license":[{"start":{"date-parts":[[1995,11,1]],"date-time":"1995-11-01T00:00:00Z","timestamp":815184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1995,11,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The 2B5 early puff locus corresponds to the Broad-Complex (BR-C) and encodes a family of transcription factors whose members are induced by the molting hormone ecdysone. Mutations in the br subcomplementation group substantially reduce the levels of Dopa decarboxylase (DDC) in the epidermis of mature third instar larvae but not in mature second instar organisms. Enzyme levels are normal in the central nervous system of the two mutants examined. The specificity of these effects suggests that a product of the BRC locus mediates the rapid appearance of DDC in mature third instar larvae experiencing an elevated titer of ecdysone. The likely identity of this protein has been confirmed by pursuing the observation that the br28 allele is caused by the insertion of a P element into the Z2 DNAbinding domain. Both the transcript and a protein carrying this domain are present in the epidermis and a BR-C recombinant protein carrying the Z2 finger binds to the first intron of the Ddc gene. Five binding sites have been identified within the intron by DNAase I footprinting and a core consensus sequence has been derived which shares some identity with the consensus binding site of the Z2 protein to the Sgs-4 regulatory region. Our demonstration that Ddc is a target of BR-C in the epidermis is the first direct evidence of a role for this early gene in a tissue other than the salivary glands. The data reinforce the idea that BR-C, which clearly mediates a salivary gland-specific response to ecdysone, may play a widespread role in the hormone\u2019s activation of gene cascades in other target tissues.<\/jats:p>","DOI":"10.1242\/dev.121.11.3913","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T02:13:32Z","timestamp":1619403212000},"page":"3913-3922","source":"Crossref","is-referenced-by-count":42,"title":["Hormonal induction of Dopa decarboxylase in the epidermis of <i>Drosophila<\/i> is mediated by the <i>Broad-Complex<\/i>"],"prefix":"10.1242","volume":"121","author":[{"given":"Ross B.","family":"Hodgetts","sequence":"first","affiliation":[{"name":"University of Alberta 1 Department of Biological Sciences , , Edmonton, AB T6G 2E9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William C.","family":"Clark","sequence":"additional","affiliation":[{"name":"University of Alberta 1 Department of Biological Sciences , , Edmonton, AB T6G 2E9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra L.","family":"O\u2019Keefe","sequence":"additional","affiliation":[{"name":"University of Alberta 1 Department of Biological Sciences , , Edmonton, AB T6G 2E9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Schouls","sequence":"additional","affiliation":[{"name":"University of Alberta 1 Department of Biological Sciences , , Edmonton, AB T6G 2E9, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kirsten","family":"Crossgrove","sequence":"additional","affiliation":[{"name":"University of Pennsylvania 2 Department of Biology , , Philadelphia PA 19104-6018, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gregory M.","family":"Guild","sequence":"additional","affiliation":[{"name":"University of Pennsylvania 2 Department of Biology , , Philadelphia PA 19104-6018, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurence von","family":"Kalm","sequence":"additional","affiliation":[{"name":"University of California 3 Department of Molecular and Cell Biology , , Berkeley, CA 94720, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[1995,11,1]]},"reference":[{"key":"2025011515152584700_DEVELOP_121_11_3913C1","first-page":"388404","article-title":"Molecular analysis of the initiation of insect metamorphosis: a comparative study of Drosophila ecdysteroid-regulated transcription","volume":"169","author":"Andres","year":"1993","journal-title":"Dev. 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