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Approximately half of the embryos died before embryonic day 11.5 and exhibited a severe defect in placental development in which the allantois failed to fuse with the chorion. The remaining VCAM-1-deficient embryos survived to embryonic day 11.5-12.5 and displayed several abnormalities in their developing hearts including a reduction of the compact layer of the ventricular myocardium and intraventricular septum. The hearts also contained significant amounts of blood in the pericardial space and lacked an epicardium. \u03b14 and VCAM-1 were found to be expressed in wild-type embryos in a reciprocal fashion in the chorion and allantois and in the epicardium and the underlying myocardium, although VCAM-1 was expressed in the intraventricular septum in the absence of adjacent \u03b14-expressing cells. These data suggest important roles for VCAM-1 and \u03b14 in the development of the placenta and the heart.<\/jats:p>","DOI":"10.1242\/dev.121.2.489","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T02:22:42Z","timestamp":1619403762000},"page":"489-503","source":"Crossref","is-referenced-by-count":474,"title":["Defective development of the embryonic and extraembryonic circulatory systems in vascular cell adhesion molecule (VCAM-1) deficient mice"],"prefix":"10.1242","volume":"121","author":[{"given":"Lia","family":"Kwee","sequence":"first","affiliation":[{"name":"1 Department of Biotechnology, Roche Research Center, Hoffmann-La Roche Inc., 340 Kingsland Street, Nutley NJ 07110-1199, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H. 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