{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T04:44:18Z","timestamp":1777610658673,"version":"3.51.4"},"reference-count":40,"publisher":"The Company of Biologists","issue":"3","license":[{"start":{"date-parts":[[1995,3,1]],"date-time":"1995-03-01T00:00:00Z","timestamp":794016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1995,3,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The cytoplasmic retinoic acid (RA)-binding protein CRABP-II is expressed widely throughout early morphogenesis in mouse embryo, but its expression becomes more restricted as organogenesis progresses. CRABP-II expression remains strong in the developing limb bud suggesting a role for this protein in limb patterning. Here, we show that the CRABP-II promoter can direct expression of a lacZ transgene in a specific posterior domain during limb bud development. In order to investigate in more detail the role played by CRABP-II in RA signal transduction, we have also generated mice homozygous for a null mutation of this gene. CRABPII-\/- mice are viable and fertile but show a developmental defect of the forelimb, specifically an additional, postaxial digit. This digit is generally, but not exclusively, limited to a single forepaw of an individual animal. The penetrance of the phenotype varies according to the genetic background, occurring most frequently on the inbred 129Sv background (50%), less frequently on the C57Bl\/6 background (30%) and rarely on the outbred CD1 background (10%). This developmental abnormality implies a role for CRABP-II in normal patterning of the limb.<\/jats:p>","DOI":"10.1242\/dev.121.3.671","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T02:08:47Z","timestamp":1619402927000},"page":"671-679","source":"Crossref","is-referenced-by-count":57,"title":["Postaxial polydactyly in forelimbs of <i>CRABP-II<\/i> mutant mice"],"prefix":"10.1242","volume":"121","author":[{"given":"Diana","family":"Fawcett","sequence":"first","affiliation":[{"name":"Division of Endocrinology 1 , Research Institute, Hospital for Sick Children, Toronto, Canada M5G 1X8"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Pasceri","sequence":"additional","affiliation":[{"name":"Division of Endocrinology 1 , Research Institute, Hospital for Sick Children, Toronto, Canada M5G 1X8"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Fraser","sequence":"additional","affiliation":[{"name":"Division of Endocrinology 1 , Research Institute, Hospital for Sick Children, Toronto, Canada M5G 1X8"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melissa","family":"Colbert","sequence":"additional","affiliation":[{"name":"Department of Microbiology 2 , Duke University School of Medicine, Durham, NC 27710"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janet","family":"Rossant","sequence":"additional","affiliation":[{"name":"Division of Molecular and Developmental Biology 3 , Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada M5G 1X5"},{"name":"Department of Molecular and Medical Genetics 4 , University of Toronto, Toronto, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vincent","family":"Gigu\u00e8re","sequence":"additional","affiliation":[{"name":"Division of Endocrinology 1 , Research Institute, Hospital for Sick Children, Toronto, Canada M5G 1X8"},{"name":"Department of Molecular and Medical Genetics 4 , University of Toronto, Toronto, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1995,3,1]]},"reference":[{"key":"2025011513464067300_DEVELOP_121_3_671C1","doi-asserted-by":"publisher","first-page":"965","DOI":"10.1083\/jcb.112.5.965","article-title":"Overexpression of the cellular retinoic acid binding protein-I (CRABP-I) results in a reduction in differentiation-specific gene expression in F9 teratocarcinoma cells","volume":"112","author":"Boylan","year":"1991","journal-title":"J. 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