{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T05:56:00Z","timestamp":1779342960689,"version":"3.51.4"},"reference-count":53,"publisher":"The Company of Biologists","issue":"9","license":[{"start":{"date-parts":[[1996,9,1]],"date-time":"1996-09-01T00:00:00Z","timestamp":841536000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1996,9,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Sex myoblast migration in the Caenorhabditis elegans hermaphrodite represents a simple, genetically amenable model system for studying how cell migration is regulated during development. Two separable components of sex myoblast guidance have been described: a gonad-independent mechanism sufficient for the initial anterior migration to the mid-body region, and a gonad-dependent mechanism required for precise final positioning (J. H. Thomas, M. J. Stern and H. R. Horvitz (1990) Cell 62, 1041-1052). Here, we demonstrate a role for a Ras-mediated signal transduction pathway in controlling sex myoblast migration. Lossof-function mutations in let-60 ras, ksr-1, lin-45 raf, let537\/mek-2 or sur-1\/mpk-1 cause defects in sex myoblast final positions that resemble those seen in gonad-ablated animals, while constitutively active let-60 ras(G13E) transgenes allow fairly precise positioning to occur in the absence of the gonad. A mosaic analysis demonstrated that let-60 ras is required within the sex myoblasts to control proper positioning. Our results suggest that gonadal signals normally stimulate let-60 ras activity in the sex myoblasts, thereby making them competent to sense or respond to positional cues that determine the precise endpoint of migration. let-60 ras may have additional roles in sex myoblast guidance as well. Finally, we have also investigated genetic interactions between let-60 ras and other genes important for sex myoblast migration, including egl15, which encodes a fibroblast growth factor receptor tyrosine kinase (D. L. DeVore, H. R. Horvitz and M. J. Stern (1995) Cell 83, 611-623). Since mutations reducing Ras pathway activity cause a different phenotype than those reducing egl-15 activity and since constitutive Ras activity only partially suppresses the migration defects of egl-15 mutants, we argue that let-60 ras and egl-15 do not act together in a single linear pathway.<\/jats:p>","DOI":"10.1242\/dev.122.9.2823","type":"journal-article","created":{"date-parts":[[2021,4,23]],"date-time":"2021-04-23T19:00:28Z","timestamp":1619204428000},"page":"2823-2833","source":"Crossref","is-referenced-by-count":52,"title":["A Ras-mediated signal transduction pathway is involved in the control of sex myoblast migration in <i>Caenorhabditis elegans<\/i>"],"prefix":"10.1242","volume":"122","author":[{"given":"Meera","family":"Sundaram","sequence":"first","affiliation":[{"name":"Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Boulder, CO 80309-0347, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Yochem","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Boulder, CO 80309-0347, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Han","sequence":"additional","affiliation":[{"name":"Department of Molecular, Cellular and Developmental Biology, University of Colorado, Boulder, Boulder, CO 80309-0347, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1996,9,1]]},"reference":[{"key":"2025021323151451300_DEVELOP_122_9_2823C1","doi-asserted-by":"publisher","first-page":"693","DOI":"10.1038\/348693a0","article-title":"The let-23 gene necessary for Caenorhabditis elegans vulval induction encodes a tyrosine kinase of the EGF receptor 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