{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T00:13:23Z","timestamp":1791332003290,"version":"4.3.1"},"reference-count":41,"publisher":"The Company of Biologists","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,5,15]]},"abstract":"<jats:p>E-cadherin-mediated cell-cell adhesion is fundamental for epithelial tissue morphogenesis, physiology and repair. E-cadherin is a core transmembrane constituent of the zonula adherens (ZA), a belt-like adherens junction located at the apicolateral border in epithelial cells. The anchorage of ZA components to cortical actin filaments strengthens cell-cell cohesion and allows for junction contractility, which shapes epithelial tissues during development. Here, we report that the cytoskeletal adaptor protein Girdin physically and functionally interacts with components of the cadherin-catenin complex during Drosophila embryogenesis. Fly Girdin is broadly expressed throughout embryonic development and enriched at the ZA in epithelial tissues. Girdin associates with the cytoskeleton and co-precipitates with the cadherin-catenin complex protein \u03b1-Catenin (\u03b1-Cat). Girdin mutations strongly enhance adhesion defects associated with reduced DE-cadherin (DE-Cad) expression. Moreover, the fraction of DE-Cad molecules associated with the cytoskeleton decreases in the absence of Girdin, thereby identifying Girdin as a positive regulator of adherens junction function. Girdin mutant embryos display isolated epithelial cell cysts and rupture of the ventral midline, consistent with defects in cell-cell cohesion. In addition, loss of Girdin impairs the collective migration of epithelial cells, resulting in dorsal closure defects. We propose that Girdin stabilizes epithelial cell adhesion and promotes morphogenesis by regulating the linkage of the cadherin-catenin complex to the cytoskeleton.<\/jats:p>","DOI":"10.1242\/dev.122002","type":"journal-article","created":{"date-parts":[[2015,5,12]],"date-time":"2015-05-12T11:20:20Z","timestamp":1431429620000},"page":"1777-1784","source":"Crossref","is-referenced-by-count":34,"title":["Girdin-mediated interactions between cadherin and the actin cytoskeleton are required for epithelial morphogenesis in <i>Drosophila<\/i>"],"prefix":"10.1242","volume":"142","author":[{"given":"Elise","family":"Houssin","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, Medical Biochemistry and Pathology\/Cancer Research Center, Laval University, and CRCHU-oncology axis, Qu\u00e9bec, Qu\u00e9bec, CanadaG1R 3S3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Tepass","sequence":"additional","affiliation":[{"name":"Department of Cell and Systems Biology, University of Toronto, Toronto, Ontario, CanadaM5S 3G5"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Laprise","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Medical Biochemistry and Pathology\/Cancer Research Center, Laval University, and CRCHU-oncology axis, Qu\u00e9bec, Qu\u00e9bec, CanadaG1R 3S3"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042608044214800_DEV122002C1","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1016\/j.bbrc.2012.08.122","article-title":"Similar phenotypes of Girdin germ-line and conditional knockout mice indicate a crucial role for Girdin in the nestin lineage","volume":"426","author":"Asai","year":"2012","journal-title":"Biochem. Biophys. Res. Commun."},{"key":"2021042608044214800_DEV122002C2","doi-asserted-by":"publisher","first-page":"761","DOI":"10.1534\/genetics.104.026427","article-title":"The BDGP gene disruption project: single transposon insertions associated with 40% of Drosophila genes","volume":"167","author":"Bellen","year":"2004","journal-title":"Genetics"},{"key":"2021042608044214800_DEV122002C3","doi-asserted-by":"publisher","first-page":"731","DOI":"10.1534\/genetics.111.126995","article-title":"The Drosophila gene disruption project: progress using transposons with distinctive site specificities","volume":"188","author":"Bellen","year":"2011","journal-title":"Genetics"},{"key":"2021042608044214800_DEV122002C4","doi-asserted-by":"crossref","first-page":"1673","DOI":"10.1093\/genetics\/144.4.1673","article-title":"The autosomal FLP-DFS technique for generating germline mosaics in Drosophila melanogaster","volume":"144","author":"Chou","year":"1996","journal-title":"Genetics"},{"key":"2021042608044214800_DEV122002C40","doi-asserted-by":"publisher","first-page":"1645","DOI":"10.1242\/dev.044107","article-title":"The PAR complex regulates pulsed actomyosin contractions during amnioserosa apical constriction in Drosophila","volume":"137","author":"David","year":"2010","journal-title":"Development"},{"key":"2021042608044214800_DEV122002C5","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1038\/ncb2685","article-title":"Monomeric alpha-catenin links cadherin to the actin cytoskeleton","volume":"15","author":"Desai","year":"2013","journal-title":"Nat. Cell Biol."},{"key":"2021042608044214800_DEV122002C6","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1016\/j.devcel.2005.08.001","article-title":"Akt\/PKB regulates actin organization and cell motility via Girdin\/APE","volume":"9","author":"Enomoto","year":"2005","journal-title":"Dev. Cell"},{"key":"2021042608044214800_DEV122002C7","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1196\/annals.1377.016","article-title":"Girdin, a novel actin-binding protein, and its family of proteins possess versatile functions in the Akt and Wnt signaling pathways","volume":"1086","author":"Enomoto","year":"2006","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"2021042608044214800_DEV122002C8","doi-asserted-by":"publisher","first-page":"774","DOI":"10.1016\/j.neuron.2009.08.015","article-title":"Roles of disrupted-in-schizophrenia 1-interacting protein girdin in postnatal development of the dentate gyrus","volume":"63","author":"Enomoto","year":"2009","journal-title":"Neuron"},{"key":"2021042608044214800_DEV122002C9","doi-asserted-by":"publisher","first-page":"2208","DOI":"10.1016\/j.cub.2005.11.064","article-title":"Nonmuscle myosin II generates forces that transmit tension and drive contraction in multiple tissues during dorsal closure","volume":"15","author":"Franke","year":"2005","journal-title":"Curr. Biol."},{"key":"2021042608044214800_DEV122002C10","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1038\/nrm2720","article-title":"Collective cell migration in morphogenesis, regeneration and cancer","volume":"10","author":"Friedl","year":"2009","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2021042608044214800_DEV122002C11","doi-asserted-by":"publisher","first-page":"487","DOI":"10.1083\/jcb.201308032","article-title":"A bidirectional antagonism between aPKC and Yurt regulates epithelial cell polarity","volume":"204","author":"Gamblin","year":"2014","journal-title":"J. Cell Biol."},{"key":"2021042608044214800_DEV122002C12","doi-asserted-by":"publisher","first-page":"381","DOI":"10.1083\/jcb.200712066","article-title":"Activation of Galphai3 triggers cell migration via regulation of GIV","volume":"182","author":"Ghosh","year":"2008","journal-title":"J. Cell Biol."},{"key":"2021042608044214800_DEV122002C13","doi-asserted-by":"publisher","first-page":"1775","DOI":"10.1534\/genetics.166.4.1775","article-title":"Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31","volume":"166","author":"Groth","year":"2004","journal-title":"Genetics"},{"key":"2021042608044214800_DEV122002C14","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1046\/j.1432-0436.2002.700408.x","article-title":"Signaling pathways directing the movement and fusion of epithelial sheets: lessons from dorsal closure in Drosophila","volume":"70","author":"Harden","year":"2002","journal-title":"Differentiation"},{"key":"2021042608044214800_DEV122002C15","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1083\/jcb.200406024","article-title":"Adherens junction-dependent and -independent steps in the establishment of epithelial cell polarity in Drosophila","volume":"167","author":"Harris","year":"2004","journal-title":"J. Cell Biol."},{"key":"2021042608044214800_DEV122002C16","doi-asserted-by":"publisher","first-page":"502","DOI":"10.1038\/nrm2927","article-title":"Adherens junctions: from molecules to morphogenesis","volume":"11","author":"Harris","year":"2010","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"2021042608044214800_DEV122002C17","doi-asserted-by":"publisher","first-page":"1310","DOI":"10.1158\/0008-5472.CAN-07-5111","article-title":"An actin-binding protein Girdin regulates the motility of breast cancer cells","volume":"68","author":"Jiang","year":"2008","journal-title":"Cancer Res."},{"key":"2021042608044214800_DEV122002C18","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1083\/jcb.149.2.471","article-title":"Multiple forces contribute to cell sheet morphogenesis for dorsal closure in Drosophila","volume":"149","author":"Kiehart","year":"2000","journal-title":"J. Cell Biol."},{"key":"2021042608044214800_DEV122002C19","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1038\/ncb1695","article-title":"Regulation of VEGF-mediated angiogenesis by the Akt\/PKB substrate Girdin","volume":"10","author":"Kitamura","year":"2008","journal-title":"Nat. Cell Biol."},{"key":"2021042608044214800_DEV122002C20","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1007\/978-94-007-4186-7_9","article-title":"Adherens junction turnover: regulating adhesion through cadherin endocytosis, degradation, and recycling","volume":"60","author":"Kowalczyk","year":"2012","journal-title":"Subcell. Biochem."},{"key":"2021042608044214800_DEV122002C21","doi-asserted-by":"publisher","first-page":"8226","DOI":"10.1074\/jbc.M110235200","article-title":"Phosphatidylinositol 3-kinase controls human intestinal epithelial cell differentiation by promoting adherens junction assembly and p38 MAPK activation","volume":"277","author":"Laprise","year":"2002","journal-title":"J. Biol. Chem."},{"key":"2021042608044214800_DEV122002C22","doi-asserted-by":"publisher","first-page":"736","DOI":"10.1091\/mbc.E10-08-0724","article-title":"Protective role of Gipie, a Girdin family protein, in endoplasmic reticulum stress responses in endothelial cells","volume":"22","author":"Matsushita","year":"2011","journal-title":"Mol. Biol. Cell"},{"key":"2021042608044214800_DEV122002C23","doi-asserted-by":"publisher","first-page":"787","DOI":"10.1083\/jcb.200812146","article-title":"Independent cadherin-catenin and Bazooka clusters interact to assemble adherens junctions","volume":"185","author":"McGill","year":"2009","journal-title":"J. Cell Biol."},{"key":"2021042608044214800_DEV122002C24","doi-asserted-by":"publisher","first-page":"509","DOI":"10.1016\/j.cell.2010.02.040","article-title":"aPKC phosphorylation of Bazooka defines the apical\/lateral border in Drosophila epithelial cells","volume":"141","author":"Morais-de-S\u00e1","year":"2010","journal-title":"Cell"},{"key":"2021042608044214800_DEV122002C25","doi-asserted-by":"publisher","first-page":"149","DOI":"10.1083\/jcb.134.1.149","article-title":"armadillo, bazooka, and stardust are critical for early stages in formation of the zonula adherens and maintenance of the polarized blastoderm epithelium in Drosophila","volume":"134","author":"Muller","year":"1996","journal-title":"J. Cell Biol."},{"key":"2021042608044214800_DEV122002C26","doi-asserted-by":"publisher","first-page":"e36681","DOI":"10.1371\/journal.pone.0036681","article-title":"Involvement of Girdin in the determination of cell polarity during cell migration","volume":"7","author":"Ohara","year":"2012","journal-title":"PLoS ONE"},{"key":"2021042608044214800_DEV122002C27","doi-asserted-by":"publisher","first-page":"2352","DOI":"10.1242\/jcs.03470","article-title":"Cooperative roles of Par-3 and afadin in the formation of adherens and tight junctions","volume":"120","author":"Ooshio","year":"2007","journal-title":"J. Cell Sci."},{"key":"2021042608044214800_DEV122002C28","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1038\/nature721","article-title":"Crumbs, the Drosophila homologue of human CRB1\/RP12, is essential for photoreceptor morphogenesis","volume":"416","author":"Pellikka","year":"2002","journal-title":"Nature"},{"key":"2021042608044214800_DEV122002C29","doi-asserted-by":"publisher","first-page":"3370","DOI":"10.1016\/j.yexcr.2009.06.019","article-title":"A novel Drosophila Girdin-like protein is involved in Akt pathway control of cell size","volume":"315","author":"Puseenam","year":"2009","journal-title":"Exp. Cell Res."},{"key":"2021042608044214800_DEV122002C30","doi-asserted-by":"publisher","first-page":"442","DOI":"10.1111\/j.1600-0854.2005.00289.x","article-title":"A novel hook-related protein family and the characterization of hook-related protein 1","volume":"6","author":"Simpson","year":"2005","journal-title":"Traffic"},{"key":"2021042608044214800_DEV122002C31","doi-asserted-by":"publisher","first-page":"1331","DOI":"10.1016\/j.cell.2009.03.050","article-title":"Pulsed forces timed by a ratchet-like mechanism drive directed tissue movement during dorsal closure","volume":"137","author":"Solon","year":"2009","journal-title":"Cell"},{"key":"2021042608044214800_DEV122002C32","doi-asserted-by":"publisher","first-page":"787","DOI":"10.1016\/0092-8674(90)90189-L","article-title":"crumbs encodes an EGF-like protein expressed on apical membranes of Drosophila epithelial cells and required for organization of epithelia","volume":"61","author":"Tepass","year":"1990","journal-title":"Cell"},{"key":"2021042608044214800_DEV122002C33","doi-asserted-by":"publisher","first-page":"672","DOI":"10.1101\/gad.10.6.672","article-title":"shotgun encodes Drosophila E-cadherin and is preferentially required during cell rearrangement in the neurectoderm and other morphogenetically active epithelia","volume":"10","author":"Tepass","year":"1996","journal-title":"Genes Dev."},{"key":"2021042608044214800_DEV122002C34","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1038\/ng1314","article-title":"A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac","volume":"36","author":"Thibault","year":"2004","journal-title":"Nat. Genet."},{"key":"2021042608044214800_DEV122002C41","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1101\/gad.10.6.659","article-title":"Zygotic Drosophila E-cadherin expression is required for processes of dynamic epithelial cell rearrangement in the Drosophila embryo","volume":"11","author":"Uemura","year":"1996","journal-title":"Genes Dev."},{"key":"2021042608044214800_DEV122002C35","doi-asserted-by":"publisher","first-page":"8109","DOI":"10.1523\/JNEUROSCI.1130-11.2011","article-title":"Girdin is an intrinsic regulator of neuroblast chain migration in the rostral migratory stream of the postnatal brain","volume":"31","author":"Wang","year":"2011","journal-title":"J. Neurosci."},{"key":"2021042608044214800_DEV122002C36","doi-asserted-by":"publisher","first-page":"836","DOI":"10.1111\/j.1349-7006.2009.01487.x","article-title":"Girding for migratory cues: roles of the Akt substrate Girdin in cancer progression and angiogenesis","volume":"101","author":"Weng","year":"2010","journal-title":"Cancer Sci."},{"key":"2021042608044214800_DEV122002C37","doi-asserted-by":"publisher","first-page":"2098","DOI":"10.15252\/embj.201488289","article-title":"Regulation of cargo-selective endocytosis by dynamin 2 GTPase-activating protein girdin","volume":"33","author":"Weng","year":"2014","journal-title":"EMBO J."},{"key":"2021042608044214800_DEV122002C38","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1038\/ncb2663","article-title":"Loss of Par3 promotes breast cancer metastasis by compromising cell-cell cohesion","volume":"15","author":"Xue","year":"2013","journal-title":"Nat. Cell Biol."},{"key":"2021042608044214800_DEV122002C39","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1101\/gad.7.1.29","article-title":"Morphogenesis in Drosophila requires nonmuscle myosin heavy chain function","volume":"7","author":"Young","year":"1993","journal-title":"Genes Dev."}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/142\/10\/1777\/1168052\/dev122002.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/142\/10\/1777\/1168052\/dev122002.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T12:40:23Z","timestamp":1619440823000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/142\/10\/1777\/46839\/Girdin-mediated-interactions-between-cadherin-and"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,5,15]]},"references-count":41,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2015,5,15]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.122002","relation":{},"ISSN":["1477-9129","0950-1991"],"issn-type":[{"value":"1477-9129","type":"electronic"},{"value":"0950-1991","type":"print"}],"subject":[],"published":{"date-parts":[[2015,5,15]]}}}