{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T23:55:10Z","timestamp":1792626910465,"version":"4.5.0"},"reference-count":49,"publisher":"The Company of Biologists","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,1,1]]},"abstract":"<jats:p>Hemimetabolous insects such as the cricket Gryllus bimaculatus regenerate lost tissue parts using blastemal cells, which is a population of dedifferentiated-proliferating cells. The gene expression of several epigenetic factors is upregulated in the blastema compared with the expression in differentiated tissue, suggesting that epigenetic changes in gene expression may control the differentiation status of blastema cells during regeneration. To clarify the molecular basis of epigenetic regulation during regeneration, we focused on the function of the Gryllus Enhancer of zeste (Gb\u2019E(z)) and Ubiquitously-transcribed tetratricopeptide repeat gene on the X chromosome (Gb\u2019Utx) homologues that regulate the methylation and demethylation on histone H3 27th lysine residue (H3K27), respectively. Methylated histone H3K27 in the regenerating leg was diminished by Gb\u2019E(z)RNAi and was increased by Gb\u2019UtxRNAi. Regenerated Gb\u2019E(z)RNAi cricket legs exhibited extra leg segment formation between the tibia and tarsus, and regenerated Gb\u2019UtxRNAi cricket legs showed leg joint formation defects in the tarsus. In the Gb\u2019E(z)RNAi-regenerating leg, the Gb\u2019dac expression domain expanded in the tarsus. In contrast, in the Gb\u2019UtxRNAi-regenerating leg, Gb\u2019Egfr expression in the middle of the tarsus was diminished. These results suggest that regulation of the histone H3K27 methylation state is involved in the repatterning process during leg regeneration among cricket species via the epigenetic regulation of leg patterning gene expression.<\/jats:p>","DOI":"10.1242\/dev.122598","type":"journal-article","created":{"date-parts":[[2015,8,8]],"date-time":"2015-08-08T01:12:41Z","timestamp":1438996361000},"source":"Crossref","is-referenced-by-count":23,"title":["Regenerated leg segment patterns are regulated epigenetically by histone H3K27 methylation in the cricket <i>Gryllus bimaculatus<\/i>"],"prefix":"10.1242","author":[{"given":"Yoshimasa","family":"Hamada","sequence":"first","affiliation":[{"name":"Graduate School of Natural Science and Technology, Okayama University, 3-1-1, Tsushima-naka, Kita-ku, Okayama city, Okayama, 700-8530, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuya","family":"Bando","sequence":"additional","affiliation":[{"name":"Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1, Shikata-cho, Kita-ku, Okayama city, Okayama, 700-8558, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taro","family":"Nakamura","sequence":"additional","affiliation":[{"name":"Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, 2-1 Minami-Jyosanjima-cho, Tokushima city, 770-8506, Japan"},{"name":"Present address; Department of Organismic and Evolutionary Biology, Harvard University,16 Divinity Avenue, BioLabs 4111, Cambridge, MA 02138, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiyasu","family":"Ishimaru","sequence":"additional","affiliation":[{"name":"Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, 2-1 Minami-Jyosanjima-cho, Tokushima city, 770-8506, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taro","family":"Mito","sequence":"additional","affiliation":[{"name":"Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, 2-1 Minami-Jyosanjima-cho, Tokushima city, 770-8506, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sumihare","family":"Noji","sequence":"additional","affiliation":[{"name":"Department of Life Systems, Institute of Technology and Science, The University of Tokushima Graduate School, 2-1 Minami-Jyosanjima-cho, Tokushima city, 770-8506, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Tomioka","sequence":"additional","affiliation":[{"name":"Graduate School of Natural Science and Technology, Okayama University, 3-1-1, Tsushima-naka, Kita-ku, Okayama city, Okayama, 700-8530, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideyo","family":"Ohuchi","sequence":"additional","affiliation":[{"name":"Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University, 2-5-1, Shikata-cho, Kita-ku, Okayama city, Okayama, 700-8558, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042608083139900_DEV122598C1","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1111\/j.1440-169X.2011.01323.x","article-title":"Survey of the differences between regenerative and non-regenerative animals","volume":"54","author":"Agata","year":"2012","journal-title":"Dev. 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