{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:15:35Z","timestamp":1789989335684,"version":"4.0.1"},"reference-count":47,"publisher":"The Company of Biologists","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,1,1]]},"abstract":"<jats:p>Formation of the kidney requires reciprocal signaling among the ureteric tubules, cap mesenchyme and surrounding stromal mesenchyme to orchestrate complex morphogenetic events. The protocadherin Fat4 influences signaling from stromal to cap mesenchyme cells to influence their differentiation into nephrons. Here we characterize the role of a putative binding partner of Fat4, the protocadherin Dchs1. Mutation of Dchs1 leads to increased numbers of cap mesenchyme cells, which are abnormally arranged around the ureteric bud tips, and impairs nephron morphogenesis. Mutation of Dchs1 also reduces branching of the ureteric bud and impairs differentiation of ureteric bud tip cells into trunk cells. Genetically, Dchs1 is required specifically within cap mesenschyme cells. The similarity of Dchs1 phenotypes to stromal-less kidneys and to Fat4 mutants implicate Dchs1 in Fat4-dependent stroma-to-cap mesenchyme signaling. Antibody staining of genetic mosaics reveals that Dchs1 protein localization is polarized within cap mesenchyme cells, where it accumulates at the interface with stromal cells, implying that it interacts directly with a stromal protein. Our observations identify a role for Fat4-Dchs1 in signaling between cell layers, implicate Dchs1 as a Fat4 receptor for stromal signaling that is essential for kidney development, and establish that vertebrate Dchs1 can be molecularly polarized in vivo.<\/jats:p>","DOI":"10.1242\/dev.122630","type":"journal-article","created":{"date-parts":[[2015,6,27]],"date-time":"2015-06-27T11:54:04Z","timestamp":1435406044000},"source":"Crossref","is-referenced-by-count":45,"title":["A Fat4-Dchs1 signal between stromal and cap mesenchyme cells influences nephrogenesis and ureteric bud branching"],"prefix":"10.1242","author":[{"given":"Yaopan","family":"Mao","sequence":"first","affiliation":[{"name":"Howard Hughes Medical Institute, Waksman Institute and Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway NJ 08854\u2008USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippa","family":"Francis-West","sequence":"additional","affiliation":[{"name":"Department of Craniofacial Development and Stem Cell Biology, King's College London, Floor 27, Guy's Tower, London SE1 9RT, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth D.","family":"Irvine","sequence":"additional","affiliation":[{"name":"Howard Hughes Medical Institute, Waksman Institute and Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway NJ 08854\u2008USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042608083813600_DEV122630C1","doi-asserted-by":"publisher","first-page":"1302","DOI":"10.1016\/j.cub.2012.05.049","article-title":"Propagation of Dachsous-fat planar cell polarity","volume":"22","author":"Ambegaonkar","year":"2012","journal-title":"Curr. 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