{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T12:23:10Z","timestamp":1791289390192,"version":"4.3.0"},"reference-count":43,"publisher":"The Company of Biologists","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2015,1,1]]},"abstract":"<jats:p>Mammalian brain development requires coordination between neural precursor proliferation, differentiation and cellular organization to create the intricate neuronal networks of the adult brain. Here, we have examined the role of the atypical cadherins Fat1 and Fat4 in this process. We show that mutation of Fat1 in mouse embryos causes defects in cranial neural tube closure, accompanied by an increase in the proliferation of cortical precursors and altered apical junctions, with perturbations in apical constriction and actin accumulation. Similarly, knockdown of Fat1 in cortical precursors by in utero electroporation leads to overproliferation of radial glial precursors. Fat1 interacts genetically with the related Fat4 cadherin to regulate these processes. Proteomic analysis reveals that Fat1 and Fat4 bind different sets of actin-regulating and junctional proteins. In vitro data suggest that Fat1 and Fat4 form cis-heterodimers, providing a mechanism for bringing together their diverse interactors. Based on these data, we propose a model in which Fat1 and Fat4 binding coordinates distinct pathways at apical junctions to regulate neural progenitor proliferation, neural tube closure and apical constriction.<\/jats:p>","DOI":"10.1242\/dev.123539","type":"journal-article","created":{"date-parts":[[2015,7,24]],"date-time":"2015-07-24T22:44:43Z","timestamp":1437777883000},"source":"Crossref","is-referenced-by-count":54,"title":["Fat1 interacts with Fat4 to regulate neural tube closure, neural progenitor proliferation and apical constriction during mouse brain development"],"prefix":"10.1242","author":[{"given":"Caroline","family":"Badouel","sequence":"first","affiliation":[{"name":"Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Zander","sequence":"additional","affiliation":[{"name":"Neuroscience and Mental Health Program, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada"},{"name":"Institute of Medical Sciences, University of Toronto, Boston MA 02115\u2008USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Liscio","sequence":"additional","affiliation":[{"name":"Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mazdak","family":"Bagherie-Lachidan","sequence":"additional","affiliation":[{"name":"Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richelle","family":"Sopko","sequence":"additional","affiliation":[{"name":"Department of Genetics, Harvard Medical School, Boston MA 02115\u2008USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Etienne","family":"Coyaud","sequence":"additional","affiliation":[{"name":"Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian","family":"Raught","sequence":"additional","affiliation":[{"name":"Princess Margaret Cancer Centre, University Health Network, Toronto, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Freda","family":"Miller","sequence":"additional","affiliation":[{"name":"Neuroscience and Mental Health Program, Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada"},{"name":"Department of Molecular Genetics, University of Toronto, Boston MA 02115\u2008USA"},{"name":"Institute of Medical Sciences, University of Toronto, Boston MA 02115\u2008USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"McNeill","sequence":"additional","affiliation":[{"name":"Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario, Canada"},{"name":"Department of Molecular Genetics, University of Toronto, Boston MA 02115\u2008USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042608061197800_DEV123539C46","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1016\/j.neuron.2005.08.037","article-title":"Evidence that embryonic neurons regulate the onset of cortical gliogenesis via cardiotrophin-1","volume":"48","author":"Barnabe-Heider","year":"2005","journal-title":"Neuron"},{"key":"2021042608061197800_DEV123539C1","doi-asserted-by":"publisher","first-page":"1218","DOI":"10.1038\/onc.2013.82","article-title":"An evolutionary shift in the regulation of the Hippo pathway between mice and flies","volume":"33","author":"Bossuyt","year":"2014","journal-title":"Oncogene"},{"key":"2021042608061197800_DEV123539C2","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1016\/j.neuron.2009.07.004","article-title":"Mammalian Par3 regulates progenitor cell asymmetric division via notch signaling in the developing neocortex","volume":"63","author":"Bultje","year":"2009","journal-title":"Neuron"},{"key":"2021042608061197800_DEV123539C3","doi-asserted-by":"publisher","first-page":"1300","DOI":"10.1038\/ng.2765","article-title":"Mutations in genes encoding the cadherin receptor-ligand pair DCHS1 and FAT4 disrupt cerebral cortical development","volume":"45","author":"Cappello","year":"2013","journal-title":"Nat. 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