{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:35:24Z","timestamp":1791311724963,"version":"4.3.1"},"reference-count":60,"publisher":"The Company of Biologists","issue":"18","license":[{"start":{"date-parts":[[1998,9,15]],"date-time":"1998-09-15T00:00:00Z","timestamp":905817600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1998,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The steroidal alkaloid cyclopamine produces cyclopia and holoprosencephaly when administered to gastrulation-stage amniote embryos. Cyclopamine-induced malformations in chick embryos are associated with interruption of Sonic hedgehog (Shh)-mediated dorsoventral patterning of the neural tube and somites. Cell types normally induced in the ventral neural tube by Shh are either absent or appear aberrantly at the ventral midline after cyclopamine treatment, while dorsal cell types normally repressed by Shh appear ventrally. Somites in cyclopamine-treated embryos show Pax7 expression throughout, indicating failure of sclerotome induction. Cyclopamine at concentrations of 20-100 nM blocks the response of neural plate explants to recombinant Shh-N in a dose-dependent manner. Similar concentrations have no effect on the post-translational modification of Shh by cholesterol in transfected COS-1 cells. Comparison of the effects of cyclopamine to those of the holoprosencephaly-inducing cholesterol synthesis inhibitor AY-9944 shows that cyclopamine does not induce malformations by interfering with cholesterol metabolism. Although AY-9944 does not interrupt Shh signaling in ovo, it blocks the response to Shh-N in explants cultured without an exogenous cholesterol source. As predicted by current models of the regulation of cholesterol metabolism, the response to Shh-N in AY-9944-treated explants is restored by providing exogenous cholesterol. However, exogenous cholesterol does not restore Shh signaling in cyclopamine-treated explants. These findings suggest that cyclopamine-induced teratogenesis is due to a more direct antagonism of Shh signal transduction.<\/jats:p>","DOI":"10.1242\/dev.125.18.3553","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T03:19:37Z","timestamp":1619407177000},"page":"3553-3562","source":"Crossref","is-referenced-by-count":579,"title":["The teratogenic <i>Veratrum<\/i> alkaloid cyclopamine inhibits Sonic hedgehog signal transduction"],"prefix":"10.1242","volume":"125","author":[{"given":"John P.","family":"Incardona","sequence":"first","affiliation":[{"name":"1 Department of Pediatrics, University of Washington, Seattle, WA 98195, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Gaffield","sequence":"additional","affiliation":[{"name":"2 Western Regional Research Center, ARS, USDA, Albany, CA 94710, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raj P.","family":"Kapur","sequence":"additional","affiliation":[{"name":"3 Department of Pathology, University of Washington, Seattle, WA, 98195, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henk","family":"Roelink","sequence":"additional","affiliation":[{"name":"4 Department of Biological Structure, University of Washington, Seattle, WA 98195, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[1998,9,15]]},"reference":[{"key":"2025021501544273200_DEVELOP_125_18_3553C1","doi-asserted-by":"publisher","first-page":"3957","DOI":"10.1073\/pnas.77.7.3957","article-title":"Mevinolin: a highly potent competitive inhibitor of hydroxymethylglutaryl-coenzyme A reductase and a cholesterol-lowering agent","volume":"77","author":"Alberts","year":"1980","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025021501544273200_DEVELOP_125_18_3553C2","doi-asserted-by":"publisher","first-page":"1755","DOI":"10.1242\/dev.121.6.1755","article-title":"Expression of zebrafish nk2.2 is influenced by sonic hedgehog\/vertebrate hedgehog-1 and demarcates a zone of neuronal differentiation in the embryonic forebrain","volume":"121","author":"Barth","year":"1995","journal-title":"Development"},{"key":"2025021501544273200_DEVELOP_125_18_3553C3","doi-asserted-by":"publisher","first-page":"2077","DOI":"10.1172\/JCI113829","article-title":"Sterol synthesis and low density lipoprotein clearance in vivo in the pregnant rat, placenta, and fetus","volume":"82","author":"Belknap","year":"1988","journal-title":"J. Clin. Invest."},{"key":"2025021501544273200_DEVELOP_125_18_3553C4","first-page":"149","article-title":"The conversion of yolk into cytoplasm in the chick blastoderm as shown by electron microscopy","volume":"6","author":"Bellairs","year":"1958","journal-title":"J. Embryol. Exp. Morph."},{"key":"2025021501544273200_DEVELOP_125_18_3553C5","first-page":"180","article-title":"A congenital cyclopian-type malformation in lambs","volume":"134","author":"Binns","year":"1959","journal-title":"J. Amer. Vet. Med. Assoc."},{"key":"2025021501544273200_DEVELOP_125_18_3553C6","first-page":"1164","article-title":"A congenital cyclopian-type malformation in lambs induced by maternal ingestion of a range plant, Veratrum californicum","volume":"24","author":"Binns","year":"1963","journal-title":"Am. J. Vet. Res."},{"key":"2025021501544273200_DEVELOP_125_18_3553C7","doi-asserted-by":"publisher","first-page":"331","DOI":"10.1016\/S0092-8674(00)80213-5","article-title":"The SREBP pathway: regulation of cholesterol metabolism by proteolysis of a membrane-bound transcription factor","volume":"89","author":"Brown","year":"1997","journal-title":"Cell"},{"key":"2025021501544273200_DEVELOP_125_18_3553C8","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1126\/science.277.5323.228","article-title":"Niemann-Pick C1 disease gene: homology to mediators of cholesterol homeostasis","volume":"277","author":"Carstea","year":"1997","journal-title":"Science"},{"key":"2025021501544273200_DEVELOP_125_18_3553C9","doi-asserted-by":"publisher","first-page":"407","DOI":"10.1038\/383407a0","article-title":"Cyclopia and defective axial patterning in mice lacking Sonic hedgehog gene function","volume":"383","author":"Chiang","year":"1996","journal-title":"Nature"},{"key":"2025021501544273200_DEVELOP_125_18_3553C10","doi-asserted-by":"publisher","first-page":"388","DOI":"10.1016\/S0022-2275(20)43065-2","article-title":"Metabolism of cholesterol in the tissues and blood of the chick embryo","volume":"10","author":"Conner","year":"1969","journal-title":"J. Lipid Res."},{"key":"2025021501544273200_DEVELOP_125_18_3553C11","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/1043-6618(95)80042-5","article-title":"Pharmacology of competitive inhibitors of HMG-CoA reductase","volume":"31","author":"Corsini","year":"1995","journal-title":"Pharmacol. Res."},{"key":"2025021501544273200_DEVELOP_125_18_3553C12","doi-asserted-by":"publisher","first-page":"29","DOI":"10.1007\/s100249900004","article-title":"Cyclopamine-induced holoprosencephaly and associated craniofacial malformations in the golden hamster: anatomic and molecular events","volume":"1","author":"Coventry","year":"1998","journal-title":"Pediatr. Dev. Path."},{"key":"2025021501544273200_DEVELOP_125_18_3553C13","doi-asserted-by":"publisher","first-page":"1555","DOI":"10.1126\/science.1350865","article-title":"Early stages of motor neuron differentiation revealed by expression of homeobox gene Islet-1","volume":"256","author":"Ericson","year":"1992","journal-title":"Science"},{"key":"2025021501544273200_DEVELOP_125_18_3553C14","doi-asserted-by":"publisher","first-page":"661","DOI":"10.1016\/S0092-8674(00)81386-0","article-title":"Two critical periods of long-range Sonic Hedgehog signaling required for the specification of motor neuron identity","volume":"87","author":"Ericson","year":"1996","journal-title":"Cell"},{"key":"2025021501544273200_DEVELOP_125_18_3553C15","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1016\/S0092-8674(00)80323-2","article-title":"Pax6 controls progenitor cell identity and neuronal fate in response to graded Shh signaling","volume":"90","author":"Ericson","year":"1997","journal-title":"Cell"},{"key":"2025021501544273200_DEVELOP_125_18_3553C16","doi-asserted-by":"publisher","first-page":"1175","DOI":"10.1016\/0092-8674(94)90009-4","article-title":"Patterning of mammalian somites by surface ectoderm and notochord: evidence for sclerotome induction by a hedgehog homolog","volume":"79","author":"Fan","year":"1994","journal-title":"Cell"},{"key":"2025021501544273200_DEVELOP_125_18_3553C17","doi-asserted-by":"publisher","first-page":"457","DOI":"10.1016\/0092-8674(95)90398-4","article-title":"Long-range sclerotome induction by Sonic hedgehog: direct role of the amino-terminal cleavage product and modulation by the cyclic AMP signaling pathway","volume":"81","author":"Fan","year":"1995","journal-title":"Cell"},{"key":"2025021501544273200_DEVELOP_125_18_3553C18","doi-asserted-by":"publisher","first-page":"286","DOI":"10.1021\/np50044a014","article-title":"Carbon-13 and proton nuclear magnetic resonance spectra of Veratrum alkaloids","volume":"49","author":"Gaffield","year":"1986","journal-title":"J. Nat. Products"},{"key":"2025021501544273200_DEVELOP_125_18_3553C19","first-page":"303","article-title":"Steroidal alkaloid teratogens: molecular probes for investigation of craniofacial malformations","volume":"15","author":"Gaffield","year":"1996","journal-title":"J. Toxicol.: Toxin Rev."},{"key":"2025021501544273200_DEVELOP_125_18_3553C20","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1016\/0092-8674(85)90078-9","article-title":"Membrane-bound domain of HMG CoA reductase is required for sterol-enhanced degradation of the enzyme","volume":"41","author":"Gil","year":"1985","journal-title":"Cell"},{"key":"2025021501544273200_DEVELOP_125_18_3553C21","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1038\/343425a0","article-title":"Regulation of the mevalonate pathway","volume":"343","author":"Goldstein","year":"1990","journal-title":"Nature"},{"key":"2025021501544273200_DEVELOP_125_18_3553C22","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1002\/jmor.1050880104","article-title":"A series of normal stages in the development of the chick embryo","volume":"88","author":"Hamburger","year":"1951","journal-title":"J. Morph."},{"key":"2025021501544273200_DEVELOP_125_18_3553C23","doi-asserted-by":"publisher","first-page":"1345","DOI":"10.1172\/JCI103182","article-title":"The distribution and chemical composition of ultracentifugally separated lipoproteins in human serum","volume":"34","author":"Havel","year":"1955","journal-title":"J. Clin. Invest."},{"key":"2025021501544273200_DEVELOP_125_18_3553C24","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1006\/dbio.1997.8511","article-title":"The function of silberblick in the positioning of the eye anlage in the zebrafish embryo","volume":"184","author":"Heisenberg","year":"1997","journal-title":"Dev. Biol."},{"key":"2025021501544273200_DEVELOP_125_18_3553C25","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1016\/S0092-8674(00)81362-8","article-title":"Sterol resistance in CHO cells traced to point mutation in SREBP cleavage-activating protein","volume":"87","author":"Hua","year":"1996","journal-title":"Cell"},{"key":"2025021501544273200_DEVELOP_125_18_3553C26","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1016\/S0925-4773(97)00097-X","article-title":"Distributions of PAX6 and PAX7 proteins suggest their involvement in both early and late phases of chick brain development","volume":"66","author":"Kawakami","year":"1997","journal-title":"Mech. Dev."},{"key":"2025021501544273200_DEVELOP_125_18_3553C27","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1002\/tera.1420010103","article-title":"Teratogenic compounds of Veratrum californicum (Durand). V. Comparison of cyclopian effects of steroidal alkaloids from the plant and structurally related compounds from other sources","volume":"1","author":"Keeler","year":"1968","journal-title":"Teratology"},{"key":"2025021501544273200_DEVELOP_125_18_3553C28","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1016\/S0031-9422(00)85817-3","article-title":"Teratogenic compounds of Veratrum californicum (Durand). VI. The structure of cyclopamine","volume":"8","author":"Keeler","year":"1969","journal-title":"Phytochemistry"},{"key":"2025021501544273200_DEVELOP_125_18_3553C29","doi-asserted-by":"publisher","first-page":"708","DOI":"10.1007\/BF02533750","article-title":"Cyclopamine and related steroidal alkaloid teratogens: Their occurrence, structural relationship, and biologic effects","volume":"13","author":"Keeler","year":"1978","journal-title":"Lipids"},{"key":"2025021501544273200_DEVELOP_125_18_3553C30","doi-asserted-by":"publisher","first-page":"478","DOI":"10.1002\/(SICI)1096-8628(19961230)66:4&lt;478::AID-AJMG22&gt;3.0.CO;2-Q","article-title":"Holoprosencephaly in RSH\/Smith-Lemli-Opitz syndrome: does abnormal cholesterol metabolism affect the function of Sonic Hedgehog?","volume":"66","author":"Kelley","year":"1996","journal-title":"Am. J. Med. Genet."},{"key":"2025021501544273200_DEVELOP_125_18_3553C31","doi-asserted-by":"publisher","first-page":"19107","DOI":"10.1074\/jbc.270.32.19107","article-title":"Molecular dissection of the role of the membrane domain in the regulated degradation of 3-hydroxy-3-methylglutaryl coenzyme A reductase","volume":"270","author":"Kumagai","year":"1995","journal-title":"J. Biol. Chem."},{"key":"2025021501544273200_DEVELOP_125_18_3553C32","doi-asserted-by":"publisher","first-page":"969","DOI":"10.1016\/0092-8674(95)90276-7","article-title":"Dorsal differentiation of neural plate cells induced by BMP-mediated signals from epidermal ectoderm","volume":"82","author":"Liem","year":"1995","journal-title":"Cell"},{"key":"2025021501544273200_DEVELOP_125_18_3553C33","doi-asserted-by":"publisher","first-page":"111","DOI":"10.1083\/jcb.126.1.111","article-title":"Characterization of caveolin-rich membrane domains isolated from an endothelial-rich source: implications for human disease","volume":"126","author":"Lisanti","year":"1994","journal-title":"J. Cell Biol."},{"key":"2025021501544273200_DEVELOP_125_18_3553C34","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1016\/S0022-2275(20)37272-2","article-title":"Normal and inhibited cholesterol synthesis in the cultured rat embryo","volume":"38","author":"Llirbat","year":"1997","journal-title":"J. Lipid Res."},{"key":"2025021501544273200_DEVELOP_125_18_3553C35","doi-asserted-by":"publisher","first-page":"232","DOI":"10.1126\/science.277.5323.232","article-title":"Murine model of Niemann-Pick C disease: mutation in a cholesterol homeostasis gene","volume":"277","author":"Loftus","year":"1997","journal-title":"Science"},{"key":"2025021501544273200_DEVELOP_125_18_3553C36","doi-asserted-by":"publisher","first-page":"3267","DOI":"10.1242\/dev.121.10.3267","article-title":"Midline signalling is required for Pax gene regulation and patterning of the eyes","volume":"121","author":"Macdonald","year":"1995","journal-title":"Development"},{"key":"2025021501544273200_DEVELOP_125_18_3553C37","doi-asserted-by":"publisher","first-page":"176","DOI":"10.1038\/384176a0","article-title":"Biochemical evidence that patched is the Hedgehog receptor","volume":"384","author":"Marigo","year":"1996","journal-title":"Nature"},{"key":"2025021501544273200_DEVELOP_125_18_3553C38","doi-asserted-by":"publisher","first-page":"322","DOI":"10.1038\/375322a0","article-title":"Requirement of 19K form of Sonic hedgehog for induction of distinct ventral cell types","volume":"375","author":"Marti","year":"1995","journal-title":"Nature"},{"key":"2025021501544273200_DEVELOP_125_18_3553C39","doi-asserted-by":"publisher","first-page":"2627","DOI":"10.1074\/jbc.271.5.2627","article-title":"Progesterone inhibits cholesterol biosynthesis in cultured cells. Accumulation of cholesterol precursors","volume":"271","author":"Metherall","year":"1996","journal-title":"J. Biol. Chem."},{"key":"2025021501544273200_DEVELOP_125_18_3553C40","doi-asserted-by":"publisher","first-page":"4153","DOI":"10.1242\/dev.124.20.4153","article-title":"Patterning of the chick forebrain anlage by the prechordal plate","volume":"124","author":"Pera","year":"1997","journal-title":"Development"},{"key":"2025021501544273200_DEVELOP_125_18_3553C41","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1038\/374363a0","article-title":"The product of hedgehog autoproteolytic cleavage active in local and long-range signalling","volume":"374","author":"Porter","year":"1995","journal-title":"Nature"},{"key":"2025021501544273200_DEVELOP_125_18_3553C42","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1126\/science.274.5285.255","article-title":"Cholesterol modification of hedgehog signaling proteins in animal development","volume":"274","author":"Porter","year":"1996","journal-title":"Science"},{"key":"2025021501544273200_DEVELOP_125_18_3553C43","doi-asserted-by":"publisher","first-page":"3887","DOI":"10.1016\/0006-2952(89)90601-1","article-title":"Combined effects of AY9944 and plasma LDL (or whole plasma) on lymphocyte blastic transformation","volume":"38","author":"Rampini","year":"1989","journal-title":"Biochem. Pharmacol."},{"key":"2025021501544273200_DEVELOP_125_18_3553C44","doi-asserted-by":"publisher","first-page":"761","DOI":"10.1016\/0092-8674(94)90514-2","article-title":"Floor plate and motor neuron induction by vhh-1, a vertebrate homolog of hedgehog expressed by the notochord","volume":"76","author":"Roelink","year":"1994","journal-title":"Cell"},{"key":"2025021501544273200_DEVELOP_125_18_3553C45","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1016\/0092-8674(95)90397-6","article-title":"Floor plate and motor neuron induction by different concentrations of the amino-terminal cleavage product of sonic hedgehog autoproteolysis","volume":"81","author":"Roelink","year":"1995","journal-title":"Cell"},{"key":"2025021501544273200_DEVELOP_125_18_3553C46","doi-asserted-by":"publisher","first-page":"357","DOI":"10.1038\/ng1196-357","article-title":"Mutations in the human Sonic Hedgehog gene cause holoprosencephaly","volume":"14","author":"Roessler","year":"1996","journal-title":"Nat. Genet."},{"key":"2025021501544273200_DEVELOP_125_18_3553C47","first-page":"1353","article-title":"Action teratogene chez le rat d\u2019un inhibiteur de la synthese du cholesterol, le AY 9944","volume":"160","author":"Roux","year":"1966","journal-title":"C. R. Seances Soc. Biol. Fil."},{"key":"2025021501544273200_DEVELOP_125_18_3553C48","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1006\/dbio.1995.1216","article-title":"Early stages of notochord and floor plate development in the chick embryo defined by normal and induced expression of HNF-3 beta","volume":"170","author":"Altaba","year":"1995","journal-title":"Dev. Biol."},{"key":"2025021501544273200_DEVELOP_125_18_3553C49","volume-title":"Holoprosencephaly: Overview and Atlas of Cases","author":"Siebert","year":"1990"},{"key":"2025021501544273200_DEVELOP_125_18_3553C50","doi-asserted-by":"publisher","first-page":"1016","DOI":"10.1126\/science.1719635","article-title":"GPI-anchored cell-surface molecules complexed to protein tyrosine kinases","volume":"254","author":"Stefanova","year":"1991","journal-title":"Science"},{"key":"2025021501544273200_DEVELOP_125_18_3553C51","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1038\/384129a0","article-title":"The tumour-suppressor gene patched encodes a candidate receptor for Sonic hedgehog","volume":"384","author":"Stone","year":"1996","journal-title":"Nature"},{"key":"2025021501544273200_DEVELOP_125_18_3553C52","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1016\/0092-8674(94)90175-9","article-title":"Hedgehog is a signaling protein with a key role in patterning Drosophila imaginal discs","volume":"76","author":"Tabata","year":"1994","journal-title":"Cell"},{"key":"2025021501544273200_DEVELOP_125_18_3553C53","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1016\/S0960-9822(95)00130-8","article-title":"Induction of motor neurons by Sonic hedgehog is independent of floor plate differentiation","volume":"5","author":"Tanabe","year":"1995","journal-title":"Curr. Biol."},{"key":"2025021501544273200_DEVELOP_125_18_3553C54","doi-asserted-by":"publisher","first-page":"1115","DOI":"10.1126\/science.274.5290.1115","article-title":"Diversity and pattern in the developing spinal cord","volume":"274","author":"Tanabe","year":"1996","journal-title":"Science"},{"key":"2025021501544273200_DEVELOP_125_18_3553C55","doi-asserted-by":"publisher","first-page":"107","DOI":"10.1056\/NEJM199401133300205","article-title":"Defective cholesterol biosynthesis associated with the Smith-Lemli-Opitz syndrome","volume":"330","author":"Tint","year":"1994","journal-title":"N. Engl. J. Med."},{"key":"2025021501544273200_DEVELOP_125_18_3553C56","doi-asserted-by":"publisher","first-page":"957","DOI":"10.1016\/0092-8674(94)90027-2","article-title":"Topographic organization of embryonic motor neurons defined by expression of LIM homeobox genes","volume":"79","author":"Tsuchida","year":"1994","journal-title":"Cell"},{"key":"2025021501544273200_DEVELOP_125_18_3553C57","doi-asserted-by":"publisher","first-page":"547","DOI":"10.1038\/382547a0","article-title":"Smoothened encodes a receptor-like serpentine protein required for hedgehog signalling","volume":"382","author":"van den Heuvel","year":"1996","journal-title":"Nature"},{"key":"2025021501544273200_DEVELOP_125_18_3553C58","doi-asserted-by":"publisher","first-page":"8460","DOI":"10.1073\/pnas.93.16.8460","article-title":"Defective forebrain development in mice lacking gp330\/megalin","volume":"93","author":"Willnow","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2025021501544273200_DEVELOP_125_18_3553C59","doi-asserted-by":"publisher","first-page":"1325","DOI":"10.1016\/S0022-2275(20)39162-8","article-title":"Changes in serum sterols of rats treated with 7-dehydrocholesterol-delta 7-reductase inhibitors: comparison to levels in humans with Smith-Lemli-Opitz syndrome","volume":"37","author":"Wolf","year":"1996","journal-title":"J. Lipid Res."},{"key":"2025021501544273200_DEVELOP_125_18_3553C60","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1016\/0092-8674(93)90248-O","article-title":"Control of cell pattern in the neural tube: motor neuron induction by diffusible factors from notochord and floor plate","volume":"73","author":"Yamada","year":"1993","journal-title":"Cell"}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/125\/18\/3553\/3139608\/develop_125_18_3553.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/125\/18\/3553\/3139608\/develop_125_18_3553.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,2,15]],"date-time":"2025-02-15T01:54:53Z","timestamp":1739584493000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/125\/18\/3553\/39897\/The-teratogenic-Veratrum-alkaloid-cyclopamine"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1998,9,15]]},"references-count":60,"journal-issue":{"issue":"18","published-print":{"date-parts":[[1998,9,15]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.125.18.3553","relation":{},"ISSN":["0950-1991","1477-9129"],"issn-type":[{"value":"0950-1991","type":"print"},{"value":"1477-9129","type":"electronic"}],"subject":[],"published":{"date-parts":[[1998,9,15]]}}}