{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,16]],"date-time":"2023-11-16T21:30:21Z","timestamp":1700170221368},"reference-count":39,"publisher":"The Company of Biologists","issue":"23","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[1998,12,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Induction of vulval fates in the C. elegans hermaphrodite is mediated by a signal transduction pathway involving Ras and MAP kinase. Previous genetic analysis has suggested that two potential targets of this pathway in the vulva precursor cells are two novel proteins, LIN-25 and SUR-2. In this report, we describe further studies of lin-25. The results of a genetic mosaic analysis together with those of experiments in which lin-25 was expressed under the control of an heterologous promoter suggest that the major focus of lin-25 during vulva induction is the vulva precursor cells themselves. We have generated antisera to LIN-25 and used these to analyse the pattern of protein expression. LIN-25 is present in all six precursor cells prior to and during vulva induction but later becomes restricted to cells of the vulval lineages. Mutations in genes in the Ras\/MAP kinase pathway do not affect the pattern of expression but the accumulation of LIN-25 is reduced in the absence of sur-2. Overexpression of LIN-25 does not rescue sur-2 mutant defects suggesting that LIN-25 and SUR-2 may function together. LIN-25 is also expressed in the lateral hypodermis. Overexpression of LIN-25 disrupts lateral hypodermal cell fusion, suggesting that lin-25 may play a role in regulating cell fusions in C. elegans.<\/jats:p>","DOI":"10.1242\/dev.125.23.4809","type":"journal-article","created":{"date-parts":[[2021,4,23]],"date-time":"2021-04-23T19:35:51Z","timestamp":1619206551000},"page":"4809-4819","source":"Crossref","is-referenced-by-count":15,"title":["<i>Caenorhabditis elegans lin-25<\/i>: cellular focus, protein expression and requirement for <i>sur-2<\/i> during induction of vulval fates"],"prefix":"10.1242","volume":"125","author":[{"given":"Lars","family":"Nilsson","sequence":"first","affiliation":[{"name":"Ume\u00e5 Center for Molecular Pathogenesis 1 , Ume\u00e5 University, S-901 87 Ume\u00e5, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiajun","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biophysics 2 , Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, NY 10032, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Teresa","family":"Tiensuu","sequence":"additional","affiliation":[{"name":"Ume\u00e5 Center for Molecular Pathogenesis 1 , Ume\u00e5 University, S-901 87 Ume\u00e5, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roy","family":"Auty","sequence":"additional","affiliation":[{"name":"Ume\u00e5 Center for Molecular Pathogenesis 1 , Ume\u00e5 University, S-901 87 Ume\u00e5, Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iva","family":"Greenwald","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biophysics 2 , Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, NY 10032, USA"},{"name":"Howard Hughes Medical Institute 3 , Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, NY 10032, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon","family":"Tuck","sequence":"additional","affiliation":[{"name":"Ume\u00e5 Center for Molecular Pathogenesis 1 , Ume\u00e5 University, S-901 87 Ume\u00e5, Sweden"},{"name":"Department of Biochemistry and Molecular Biophysics 2 , Columbia University College of Physicians and Surgeons, 701 West 168th Street, New York, NY 10032, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[1998,12,1]]},"reference":[{"key":"2023090710502592400_DEVELOP_125_23_4809C1","doi-asserted-by":"crossref","first-page":"693","DOI":"10.1038\/348693a0","article-title":"The let-23 gene necessary for Caenorhabditis elegans vulval induction encodes a tyrosine kinase of the EGF receptor 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