{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T06:52:18Z","timestamp":1791528738483,"version":"4.3.4"},"reference-count":44,"publisher":"The Company of Biologists","issue":"1","license":[{"start":{"date-parts":[[2000,1,1]],"date-time":"2000-01-01T00:00:00Z","timestamp":946684800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,1,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The 90 kDa heat-shock proteins (HSP90s) play important roles during stress situations as general chaperones and under physiological conditions in the conformational activation of specific protein substrates. Vertebrates express two cytosolic HSP90s (HSP90\u03b1 and HSP90\u03b2) ubiquitously. We have mutated the Hsp90\u03b2 gene in murine embryonic stem cells and generated Hsp90\u03b2 mutant mice. Heterozygous animals were phenotypically normal. Interestingly, homozygous embryos developed normally until embryonic day 9.0\/9.5. Then, although Hsp90\u03b2 is expressed ubiquitously, they exhibited phenotypic abnormalities restricted to the placenta. The mutant concepti failed to form a fetal placental labyrinth and died a day later. Fusion between the allantois and the chorionic plate occurred, allantoic blood vessels invaded the chorion, but then did not expand. Mutant trophoblast cells failed to differentiate into trilaminar labyrinthine trophoblast.<\/jats:p>\n               <jats:p>Despite conspicuous similarities between HSP90\u03b1 and HSP90\u03b2 at the molecular level, our data suggest that HSP90\u03b2 has a key role in placenta development that cannot be performed by the endogenous HSP90\u03b1 alone. Analysis of chimeric concepti consisting of mutant embryos and tetraploid embryos or ES cells revealed that wild-type allantois was able to induce mutant trophoblast to differentiate. In contrast, trophoblast wild type at the Hsp90\u03b2 locus was unable to differentiate when in contact with mutant allantois. Therefore, the primary defect caused by the Hsp90\u03b2 mutation resided in the allantois. The allantois mesoderm is thought to induce trophoblast differentiation. Our results show that Hsp90\u03b2 is a necessary component of this induction process.<\/jats:p>","DOI":"10.1242\/dev.127.1.1","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T04:01:36Z","timestamp":1619409696000},"page":"1-11","source":"Crossref","is-referenced-by-count":259,"title":["Mice lacking HSP90\u03b2 fail to develop a placental labyrinth"],"prefix":"10.1242","volume":"127","author":[{"given":"Anne K.","family":"Voss","sequence":"first","affiliation":[{"name":"Max Planck Institute of Biophysical Chemistry Department of Molecular Cell Biology, , Am Fassberg 11, 37077 Goettingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim","family":"Thomas","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Biophysical Chemistry Department of Molecular Cell Biology, , Am Fassberg 11, 37077 Goettingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Gruss","sequence":"additional","affiliation":[{"name":"Max Planck Institute of Biophysical Chemistry Department of Molecular Cell Biology, , Am Fassberg 11, 37077 Goettingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2000,1,1]]},"reference":[{"key":"2023090515053231200_DEVELOP_127_1_1C1","doi-asserted-by":"publisher","first-page":"2977","DOI":"10.1128\/jb.170.7.2977-2983.1988","article-title":"Ancient heat shock gene is dispensable","volume":"170","author":"Bardwell","year":"1988","journal-title":"J. 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