{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T23:56:21Z","timestamp":1791158181327,"version":"4.1.0"},"reference-count":74,"publisher":"The Company of Biologists","issue":"10","license":[{"start":{"date-parts":[[2000,5,15]],"date-time":"2000-05-15T00:00:00Z","timestamp":958348800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>dHAND is a basic helix-loop-helix (bHLH) transcription factor essential for cardiovascular development. Here we analyze its pattern of expression and functional role during chick limb development. dHAND expression was observed in the lateral plate mesoderm prior to emergence of the limb buds. Coincident with limb initiation, expression of dHAND became restricted to the posterior half of the limb bud. Experimental procedures that caused mirror-image duplications of the limb resulted in mirror-image duplications of the pattern of dHAND expression along the anterior-posterior axis. Retroviral overexpression of dHAND in the limb bud produced preaxial polydactyly, corresponding to mild polarizing activity at the anterior border. At the molecular level, misexpression of dHAND caused ectopic activation of members of the Sonic hedgehog (Shh) pathway, including Gli and Patched, in the anterior limb bud. A subset of infected embryos displayed ectopic anterior activation of Shh. Other factors implicated in anterior-posterior polarization of the bud such as the most 5\u2032 Hoxd genes and Bmp2 were also ectopically activated at the anterior border. Our results indicate a role for dHAND in the establishment of anterior-posterior polarization of the limb bud.<\/jats:p>","DOI":"10.1242\/dev.127.10.2133","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T04:05:34Z","timestamp":1619409934000},"page":"2133-2142","source":"Crossref","is-referenced-by-count":148,"title":["Role of dHAND in the anterior-posterior polarization of the limb bud: implications for the Sonic hedgehog pathway"],"prefix":"10.1242","volume":"127","author":[{"given":"M.","family":"Fernandez-Teran","sequence":"first","affiliation":[{"name":"1 Departamento de Anatom\u00eda y Biolog\u00eda Celular, Universidad de Cantabria, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. E.","family":"Piedra","sequence":"additional","affiliation":[{"name":"1 Departamento de Anatom\u00eda y Biolog\u00eda Celular, Universidad de Cantabria, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I. S.","family":"Kathiriya","sequence":"additional","affiliation":[{"name":"2 Departments of Pediatrics and Molecular Biology, University of Texas Southwestern Medical Center, Dallas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Srivastava","sequence":"additional","affiliation":[{"name":"2 Departments of Pediatrics and Molecular Biology, University of Texas Southwestern Medical Center, Dallas, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. C.","family":"Rodriguez-Rey","sequence":"additional","affiliation":[{"name":"3 Departamento de Biolog\u00eda Molecular, Unidad Asociada al Centro de Investigaciones Biol\u00f3gicas, CSIC, Universidad de Cantabria, 39011 Santander, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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