{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T01:38:15Z","timestamp":1791077895855,"version":"4.1.0"},"reference-count":37,"publisher":"The Company of Biologists","issue":"17","license":[{"start":{"date-parts":[[2000,9,1]],"date-time":"2000-09-01T00:00:00Z","timestamp":967766400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,9,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Patterning of the gut into morphologically distinct regions results from the appropriate factors being expressed in strict spatial and temporal patterns to assign cells their fates in development. Often, the boundaries of gene expression early in development correspond to delineations between different regions of the adult gut. For example, Bmp4 is expressed throughout the hindgut and midgut, but is not expressed in the early gizzard. Ectopic BMP4 in the gizzard caused a thinning of the muscularis. To understand this phenotype we examined the expression of the receptors transducing BMP signaling during gut development. We find that the BMP receptors are differentially expressed in distinct regions of the chicken embryonic gut. By using constitutively activated versions of the BMP type I receptors, we find that the BMP receptors act similarly to BMP4 in the gizzard when ectopically expressed. We show that the mesodermal thinning seen upon ectopic BMP signaling is due to an increase in apoptosis and a decrease in proliferation within the gizzard mesoderm. The mesodermal thinning is characterized by a disorganization and lack of differentiation of smooth muscle in the gizzard mesoderm. Further, ectopic BMP receptors cause an upregulation of Nkx2.5, the pyloric sphincter marker, similar to that seen with ectopic BMP4. This upregulation of Nkx2.5 is a cell-autonomous event within the mesoderm of the gizzard. We also find that Nkx2.5 is necessary and sufficient for establishing aspects of pyloric sphincter differentiation.<\/jats:p>","DOI":"10.1242\/dev.127.17.3671","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T04:18:21Z","timestamp":1619410701000},"page":"3671-3681","source":"Crossref","is-referenced-by-count":84,"title":["Roles of BMP signaling and Nkx2.5 in patterning at the chick midgut-foregut boundary"],"prefix":"10.1242","volume":"127","author":[{"given":"Devyn M.","family":"Smith","sequence":"first","affiliation":[{"name":"1 Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corinne","family":"Nielsen","sequence":"additional","affiliation":[{"name":"2 Department of Pathology, Massachusetts General Hospital, Boston, MA 02114, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clifford J.","family":"Tabin","sequence":"additional","affiliation":[{"name":"1 Department of Genetics, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Drucilla J.","family":"Roberts","sequence":"additional","affiliation":[{"name":"2 Department of Pathology, Massachusetts General Hospital, Boston, MA 02114, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2000,9,1]]},"reference":[{"key":"2023032901203717500_","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1016\/S0960-9822(06)00340-X","article-title":"Sonic hedgehog directs specialised mesoderm differentiation in the intestine and pancreas","volume":"7","author":"Apelqvist","year":"1997","journal-title":"Curr. 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