{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T14:10:41Z","timestamp":1791295841550,"version":"4.1.0"},"reference-count":53,"publisher":"The Company of Biologists","issue":"19","license":[{"start":{"date-parts":[[2000,10,1]],"date-time":"2000-10-01T00:00:00Z","timestamp":970358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,10,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Using genetic and cytogenetic markers, we assessed early development and X-chromosome inactivation (X-inactivation) in XX mouse androgenones produced by pronuclear transfer. Contrary to the current view, XX androgenones are capable of surviving to embryonic day 7.5, achieving basically random X-inactivation in all tissues including those derived from the trophectoderm and primitive endoderm that are characterized by paternal X-activation in fertilized embryos. This finding supports the hypothesis that in fertilized female embryos, the maternal X chromosome remains active until the blastocyst stage because of a rigid imprint that prevents inactivation, whereas the paternal X chromosome is preferentially inactivated in extra-embryonic tissues owing to lack of such imprint. In spite of random X-inactivation in XX androgenones, FISH analyses revealed expression of stable Xist RNA from every X chromosome in XX and XY androgenonetic embryos from the four-cell to morula stage. Although the occurrence of inappropriate X-inactivation was further suggested by the finding that Xist continues ectopic expression in a proportion of cells from XX and XY androgenones at the blastocyst and the early egg cylinder stage, a replication banding study failed to provide positive evidence for inappropriate X-inactivation at E6.5.<\/jats:p>","DOI":"10.1242\/dev.127.19.4137","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T04:15:19Z","timestamp":1619410519000},"page":"4137-4145","source":"Crossref","is-referenced-by-count":59,"title":["X-chromosome inactivation in XX androgenetic mouse embryos surviving implantation"],"prefix":"10.1242","volume":"127","author":[{"given":"Ikuhiro","family":"Okamoto","sequence":"first","affiliation":[{"name":"Hokkaido University 1 Division of Bioscience, Graduate School of Environmental Earth Science , , Sapporo 0600810, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seong-Seng","family":"Tan","sequence":"additional","affiliation":[{"name":"The University of Melbourne, Parkville 3 Developmental Biology Laboratory, Howard Florey Institute , , Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuo","family":"Takagi","sequence":"additional","affiliation":[{"name":"Hokkaido University 1 Division of Bioscience, Graduate School of Environmental Earth Science , , Sapporo 0600810, Japan"},{"name":"Hokkaido University 2 Research Center for Molecular Genetics , , Sapporo 0600810, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2000,10,1]]},"reference":[{"key":"2024011016304059500_DEVELOP_127_19_4137C1","doi-asserted-by":"publisher","first-page":"77","DOI":"10.1017\/S0016672300027944","article-title":"The influence of the Robertsonian translocation Rb(X.2)2Ad on anaphase non-disjunction in male laboratory mice","volume":"53","author":"Adler","year":"1989","journal-title":"Genet. 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