{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:39:48Z","timestamp":1791301188963,"version":"4.1.0"},"reference-count":43,"publisher":"The Company of Biologists","issue":"23","license":[{"start":{"date-parts":[[2000,12,1]],"date-time":"2000-12-01T00:00:00Z","timestamp":975628800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000,12,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>FTZ-F1, a member of the nuclear receptor superfamily, has been implicated in the activation of the segmentation gene fushi tarazu during early embryogenesis of Drosophila melanogaster. We found that an isoform of FTZ-F1, \u03b2FTZ-F1, is expressed in the nuclei of almost all tissues slightly before the first and second larval ecdysis and before pupation. Severely affected ftz-f1 mutants display an embryonic lethal phenotype, but can be rescued by ectopic expression of \u03b2FTZ-F1 during the period of endogenous \u03b2FTZ-F1 expression in the wild type. The resulting larvae are not able to molt, but this activity is rescued again by forced expression of \u03b2FTZ-F1, allowing progression to the next larval instar stage. On the other hand, premature expression of \u03b2FTZ-F1 in wild-type larvae at mid-first instar or mid-second instar stages causes defects in the molting process. Sensitive periods were found to be around the time of peak ecdysteroid levels and slightly before the start of endogenous \u03b2FTZ-F1 expression. A hypomorphic ftz-f1 mutant that arrests in the prepupal stage can also be rescued by ectopic, time-specific expression of \u03b2FTZ-F1. Failure of salivary gland histolysis, one of the phenotypes of the ftz-f1 mutant, is rescued by forced expression of the ftz-f1 downstream gene BR-C during the late prepupal period. These results suggest that \u03b2FTZ-F1 regulates genes associated with ecdysis and metamorphosis, and that the exact timing of its action in the ecdysone-induced gene cascade is important for proper development.<\/jats:p>","DOI":"10.1242\/dev.127.23.5083","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T04:24:25Z","timestamp":1619411065000},"page":"5083-5092","source":"Crossref","is-referenced-by-count":142,"title":["Temporally restricted expression of transcription factor \u03b2FTZ-F1: significance for embryogenesis, molting and metamorphosis in <i>Drosophila melanogaster<\/i>"],"prefix":"10.1242","volume":"127","author":[{"given":"Masa-aki","family":"Yamada","sequence":"first","affiliation":[{"name":"1 Department of Genetics, The Graduate University for Advanced Studies, and Department of Developmental Genetics, National Institute of Genetics, Mishima, Shizuoka-ken 411-8540, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takehide","family":"Murata","sequence":"additional","affiliation":[{"name":"1 Department of Genetics, The Graduate University for Advanced Studies, and Department of Developmental Genetics, National Institute of Genetics, Mishima, Shizuoka-ken 411-8540, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susumu","family":"Hirose","sequence":"additional","affiliation":[{"name":"1 Department of Genetics, The Graduate University for Advanced Studies, and Department of Developmental Genetics, National Institute of Genetics, Mishima, Shizuoka-ken 411-8540, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Lavorgna","sequence":"additional","affiliation":[{"name":"2 Laboratory of Molecular Cell Biology, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892-4255, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emiko","family":"Suzuki","sequence":"additional","affiliation":[{"name":"3 Department of Fine Morphology, Institute of Medical Science, University of Tokyo, 4-6-1, Shirokanedai, Minato-ku, Tokyo 108-8639, Japan"},{"name":"4CREST, Japan Science and Technology Corporation"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitoshi","family":"Ueda","sequence":"additional","affiliation":[{"name":"1 Department of Genetics, The Graduate University for Advanced Studies, and Department of Developmental Genetics, National Institute of Genetics, Mishima, Shizuoka-ken 411-8540, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2000,12,1]]},"reference":[{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"388","DOI":"10.1006\/dbio.1993.1315","article-title":"Molecular analysis of the initiation of insect metamorphosis: A comparative study of Drosophila ecdysteroid-regulated transcription","volume":"160","author":"Andres","year":"1993","journal-title":"Dev. Biol"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"132","DOI":"10.1016\/0168-9525(92)90079-J","article-title":"Hormones, puffs and flies: the molecular control of metamorphosis by ecdysone","volume":"8","author":"Andres","year":"1992","journal-title":"Trends Genet"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"569","DOI":"10.1016\/0012-1606(91)90257-4","article-title":"20-hydroxyecdysone is required for, negatively regulates, transcription of Drosophila pupal cuticle protein genes","volume":"146","author":"Apple","year":"1991","journal-title":"Dev. Biol"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"255","DOI":"10.1007\/BF00290925","article-title":"Patterns of puffing activity in the salivary gland chromosome of Drosophila VI. Induction by ecdysone in salivary glands of D. melanogaster cultured in vitro","volume":"38","author":"Ashburner","year":"1972","journal-title":"Chromosoma"},{"key":"2023030307470042400_","volume-title":"Drosophila: A Laboratory Handbook","author":"Ashburner","year":"1989"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1101\/SQB.1974.038.01.070","article-title":"Temporal control of puffing activity in polytene chromosome","volume":"38","author":"Ashburner","year":"1974","journal-title":"Cold Spring Harbor Symp. Quant. Biol"},{"key":"2023030307470042400_","first-page":"57","article-title":"Staging the metamorphosis of Drosophila melanogaster","volume":"66","author":"Bainbridge","year":"1981","journal-title":"J. Embryol. Exp. Morph"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1006\/dbio.1997.8620","article-title":"Relationships between protein isoforms and genetic functions demonstrate functional redundancy at the Broad-Complex during Drosophila metamorphosis","volume":"187","author":"Bayer","year":"1997","journal-title":"Dev. Biol"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"654","DOI":"10.1007\/BF00396591","article-title":"Die puffs der speicheldr\u00fcsenchromosomen von Drosophila melanogaster","volume":"10","author":"Becker","year":"1959","journal-title":"Chromosoma"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"777","DOI":"10.1016\/S0092-8674(00)80466-3","article-title":"Drosophila ecdysone receptor mutations reveal functional differences among receptor isoforms","volume":"91","author":"Bender","year":"1997","journal-title":"Cell"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1016\/S1097-2765(00)80305-6","article-title":"The Drosophila \u03b2FTZ-F1 orphan nuclear receptor provides competence for stage-specific responses to the steroid hormone ecdysone","volume":"3","author":"Broadus","year":"1999","journal-title":"Mol. Cell"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"12024","DOI":"10.1073\/pnas.94.22.12024","article-title":"DHR3, an ecdysone-inducible early-late gene encoding a Drosophila nuclear receptor, is required for embryogenesis","volume":"94","author":"Carney","year":"1997","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1083\/jcb.101.1.189","article-title":"The pupal cuticle of Drosophila; Biphasic synthesis of pupal cuticle proteins in vivo and in vitro in response to 20-hydroxyecdysone","volume":"101","author":"Doctor","year":"1985","journal-title":"J. Cell Biol"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"3275","DOI":"10.1242\/dev.120.11.3275","article-title":"Differential expression of Broad-Complex transcription factors may forecast tissue-specific developmental fates during Drosophila metamorphosis","volume":"120","author":"Emery","year":"1994","journal-title":"Development"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"1411","DOI":"10.1242\/dev.121.5.1411","article-title":"The Drosophila E74 gene is required for the proper stage- and tissue-specific transcription of ecdysone-regulated genes at the onset of metamorphosis","volume":"121","author":"Fletcher","year":"1995","journal-title":"Development"},{"key":"2023030307470042400_","first-page":"843","article-title":"The Metamorphic Development of the Adult Epidermis","volume-title":"The Development of Drosophila melanogaster","author":"Fristrom","year":"1993"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"548","DOI":"10.1038\/385548a0","article-title":"The nuclear receptor homologue Ftz-F1 and the homeodomain protein Ftz are mutually dependent cofactors","volume":"385","author":"Guichet","year":"1997","journal-title":"Nature"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"4709","DOI":"10.1242\/dev.125.23.4709","article-title":"The RXR homolog Ultraspiracle is an essential component of the Drosophila ecdysone receptor","volume":"125","author":"Hall","year":"1998","journal-title":"Development"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1242\/dev.118.1.105","article-title":"Control of imaginal cell development by the escargot gene of Drosophila","volume":"118","author":"Hayashi","year":"1993","journal-title":"Development"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"4673","DOI":"10.1242\/dev.124.22.4673","article-title":"Steroid regulated programmed cell death during Drosophila metamorphosis","volume":"124","author":"Jiang","year":"1997","journal-title":"Development"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"4083","DOI":"10.1002\/j.1460-2075.1992.tb05501.x","article-title":"Temporal coordination of regulatory gene expression by the steroid hormone ecdysone","volume":"11","author":"Karim","year":"1992","journal-title":"EMBO J"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1016\/0022-1910(85)90073-3","article-title":"Cuticle secretion during larval growth in Drosophila melanogaster","volume":"31","author":"Kaznowski","year":"1985","journal-title":"J. Insect. Physiol"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"247","DOI":"10.1093\/genetics\/118.2.247","article-title":"Interaction and developmental effects of mutations in the Broad-Complex of the Drosophila melanogaster","volume":"118","author":"Kiss","year":"1988","journal-title":"Genetics"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"3004","DOI":"10.1073\/pnas.90.7.3004","article-title":"Potential role for a FTZ-F1 steroid receptor superfamily member in the control of Drosophila metamorphosis","volume":"90","author":"Lavorgna","year":"1993","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"848","DOI":"10.1126\/science.1709303","article-title":"FTZ-F1, a steroid hormone receptor-like protein implicated in the activation of fushi tarazu","volume":"252","author":"Lavorgna","year":"1991","journal-title":"Science"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"2897","DOI":"10.1242\/dev.127.13.2897","article-title":"A conditional rescue system reveals essential functions for the ecdysone receptor (EcR) gene during molting and metamorphosis in Drosophila","volume":"127","author":"Li","year":"2000","journal-title":"Development"},{"key":"2023030307470042400_","volume-title":"The genome of","author":"Lindsley","year":"1992"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"6509","DOI":"10.1128\/MCB.16.11.6509","article-title":"Regulation of the EDG84A gene by FTZ-F1 during metamorphosis in Drosophila melanogaster","volume":"16","author":"Murata","year":"1996","journal-title":"Mol. Cell. Biol"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"174","DOI":"10.1016\/0012-1606(91)90328-Z","article-title":"Mutations in a steroid hormone-regulated gene disrupt the metamorphosis of the central nervous system in Drosophila","volume":"148","author":"Restifo","year":"1991","journal-title":"Dev. Biol"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1007\/BF00188753","article-title":"Mutation in a steroid hormone-regulated gene disrupt the metamorphosis of internal tissues in Drosophila: salivary glands, muscle, and gut","volume":"201","author":"Restifo","year":"1992","journal-title":"Wilhelm Roux\u2019s Arch. Dev. Biol"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"264","DOI":"10.1016\/0012-1606(76)90304-3","article-title":"Sequential gene activation by ecdysone in polytene chromosomes of Drosophila melanogaster IV. The mid prepupal period","volume":"54","author":"Richards","year":"1976","journal-title":"Dev. Biol"},{"key":"2023030307470042400_","first-page":"899","article-title":"Hormones and Drosophila Development","volume-title":"The Development of Drosophila melanogaster","author":"Riddiford","year":"1993"},{"key":"2023030307470042400_","volume-title":"Molecular Cloning: A Laboratory Manual","author":"Sambrook","year":"1989"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"2053","DOI":"10.1242\/dev.125.11.2053","article-title":"Drosophila EcR-B ecdysone receptor isoforms are required for larval molting and for neuron remodeling during metamorphosis","volume":"125","author":"Schubiger","year":"1998","journal-title":"Development"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"426","DOI":"10.1006\/dbio.1994.1099","article-title":"Intermittent Expression of BmFTZ-F1, a member of the nuclear hormone receptor superfamily during development of the silkworm Bombyx mori","volume":"162","author":"Sun","year":"1994","journal-title":"Dev. Biol"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"871","DOI":"10.1016\/0092-8674(95)90203-1","article-title":"From embryogenesis to metamorphosis: The regulation and function of Drosophila nuclear receptor superfamily members","volume":"83","author":"Thummel","year":"1995","journal-title":"Cell"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"306","DOI":"10.1016\/0168-9525(96)10032-9","article-title":"Flies on steroids-Drosophila metamorphosis and the mechanisms of steroid hormone action","volume":"12","author":"Thummel","year":"1996","journal-title":"Trends Genet"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"7229","DOI":"10.1093\/nar\/18.24.7229","article-title":"Identification and purification of a Bombyx mori homologue of FTZ-F1","volume":"18","author":"Ueda","year":"1990","journal-title":"Nucl. Acids Res"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"624","DOI":"10.1101\/gad.4.4.624","article-title":"A sequence-specific DNA-binding protein that activates fushi tarazu segmentation gene expression","volume":"4","author":"Ueda","year":"1990","journal-title":"Genes Dev"},{"key":"2023030307470042400_","first-page":"5667","article-title":"A novel-DNA binding motif abuts the zinc finger domain of insect nuclear hormone receptor FTZ-F1 and mouse embryonal long terminal repeat-binding protein","volume":"12","author":"Ueda","year":"1992","journal-title":"Mol. Cell. Biol"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"306","DOI":"10.1083\/jcb.102.1.306","article-title":"The Pupal Cuticle of Drosophila. Differential ultrastructural immunolocalization of cuticle proteins","volume":"102","author":"Wolfgang","year":"1986","journal-title":"J. Cell. Biol"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"607","DOI":"10.1016\/0092-8674(94)90546-0","article-title":"A molecular mechanism for the stage specificity of the Drosophila prepupal genetic response to ecdysone","volume":"79","author":"Woodard","year":"1994","journal-title":"Cell"},{"key":"2023030307470042400_","doi-asserted-by":"publisher","first-page":"552","DOI":"10.1038\/385552a0","article-title":"The nuclear hormone receptor Ftz-F1 is a cofactor for the Drosophila homeodomain protein Ftz","volume":"385","author":"Yu","year":"1997","journal-title":"Nature"}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/127\/23\/5083\/2590493\/develop_127_23_5083.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/127\/23\/5083\/2590493\/develop_127_23_5083.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,3,3]],"date-time":"2023-03-03T07:47:45Z","timestamp":1677829665000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/127\/23\/5083\/41084\/Temporally-restricted-expression-of-transcription"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,12,1]]},"references-count":43,"journal-issue":{"issue":"23","published-print":{"date-parts":[[2000,12,1]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.127.23.5083","relation":{},"ISSN":["0950-1991","1477-9129"],"issn-type":[{"value":"0950-1991","type":"print"},{"value":"1477-9129","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,12,1]]}}}