{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T20:29:32Z","timestamp":1791232172170,"version":"4.1.0"},"reference-count":78,"publisher":"The Company of Biologists","issue":"10","license":[{"start":{"date-parts":[[2001,5,15]],"date-time":"2001-05-15T00:00:00Z","timestamp":989884800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,5,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Loss-of-function analysis has shown that the transforming growth factor-like signaling molecule nodal is essential for mouse mesoderm development. However, definitive proof of nodal function in other developmental processes in the mouse embryo has been lacking because the null mutation blocks gastrulation. We describe the generation and analysis of a hypomorphic nodal allele. Mouse embryos heterozygous for the hypomorphic allele and a null allele undergo gastrulation but then display abnormalities that fall into three distinct mutant phenotypic classes, which may result from expression levels falling below critical thresholds in one or more domains of nodal expression. Our analysis of each of these classes provides conclusive evidence for nodal-mediated regulation of several developmental processes in the mouse embryo, beyond its role in mesoderm formation. We find that nodal signaling is required for correct positioning of the anteroposterior axis, normal anterior and midline patterning, and the left- right asymmetric development of the heart, vasculature, lungs and stomach.<\/jats:p>","DOI":"10.1242\/dev.128.10.1831","type":"journal-article","created":{"date-parts":[[2021,4,23]],"date-time":"2021-04-23T20:04:52Z","timestamp":1619208292000},"page":"1831-1843","source":"Crossref","is-referenced-by-count":302,"title":["Genetic dissection of<i>nodal<\/i>function in patterning the mouse embryo"],"prefix":"10.1242","volume":"128","author":[{"given":"Linda A.","family":"Lowe","sequence":"first","affiliation":[{"name":"Experimental Immunology Branch, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Satoru","family":"Yamada","sequence":"additional","affiliation":[{"name":"Experimental Immunology Branch, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael R.","family":"Kuehn","sequence":"additional","affiliation":[{"name":"Experimental Immunology Branch, Division of Basic Sciences, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2001,5,15]]},"reference":[{"key":"2023022722554138900_","doi-asserted-by":"publisher","first-page":"1173","DOI":"10.1242\/dev.127.6.1173","article-title":"Endodermal Nodal-related signals and mesoderm induction in Xenopus","volume":"127","author":"Agius","year":"2000","journal-title":"Development"},{"key":"2023022722554138900_","doi-asserted-by":"publisher","first-page":"658","DOI":"10.1038\/35001072","article-title":"The organizer factors Chordin and Noggin are required for mouse forebrain development","volume":"403","author":"Bachiller","year":"2000","journal-title":"Nature"},{"key":"2023022722554138900_","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1016\/S0168-9525(98)01499-1","article-title":"Anterior patterning in mouse","volume":"14","author":"Beddington","year":"1998","journal-title":"Trends Genet"},{"key":"2023022722554138900_","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1016\/S0092-8674(00)80560-7","article-title":"Axis development and early asymmetry in mammals","volume":"96","author":"Beddington","year":"1999","journal-title":"Cell"},{"key":"2023022722554138900_","doi-asserted-by":"publisher","first-page":"45","DOI":"10.1016\/S0925-4773(97)00125-1","article-title":"Cerberus-like is a secreted factor with neutralizing activity expressed in the anterior primitive endoderm of the mouse gastrula","volume":"68","author":"Belo","year":"1997","journal-title":"Mech. 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