{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T16:15:55Z","timestamp":1789661755464,"version":"4.0.1"},"reference-count":40,"publisher":"The Company of Biologists","issue":"18","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,9,15]]},"abstract":"<jats:p>The invariant cell lineage of nematodes allows the formation of organ systems, like the egg-laying system, to be studied at a single cell level. The Caenorhabditis elegans egg-laying system is made up of the vulva, the mesodermal gonad and muscles and several neurons. The gonad plays a central role in patterning the underlying ectoderm to form the vulva and guiding the migration of the sex myoblasts to their final position. In Pristionchus pacificus, the egg-laying system is homologous to C. elegans, but comparative studies revealed several differences at the cellular and molecular levels during vulval formation. For example, the mesoblast M participates in lateral inhibition, a process that influences the fate of two vulval precursor cells. Here, we describe the M lineage in Pristionchus and show that both the dorsal and ventral M sublineages are involved in lateral inhibition. Mutations in the homeotic gene Ppa-mab-5 cause severe misspecification of the M lineage, resembling more the C. elegans Twist than the mab-5 phenotype. Ectopic differentiation of P8.p in Ppa-mab-5 results from at least two separate interactions between M and P8.p. Thus, interactions among the Pristionchus egg-laying system are complex, involving multiple cells of different tissues occurring over a distance.<\/jats:p>","DOI":"10.1242\/dev.128.18.3395","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T02:00:58Z","timestamp":1619402458000},"page":"3395-3404","source":"Crossref","is-referenced-by-count":10,"title":["Formation of the egg-laying system in\n                    <i>Pristionchus pacificus<\/i>\n                    requires complex interactions between gonadal, mesodermal and epidermal tissues and does not rely on single cell inductions"],"prefix":"10.1242","volume":"128","author":[{"given":"Benno","family":"Jungblut","sequence":"first","affiliation":[{"name":"Present address: Department of Biochemistry and Biophysics, UC San Francisco, San Francisco, CA 94143, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre\u0301","family":"Pires-daSilva","sequence":"additional","affiliation":[{"name":"Max-Planck Institut fu\u0308r Entwicklungsbiologie, Abteilung Evolutionsbiologie, Spemannstrasse 37-39, D-72076 Tu\u0308bingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf J.","family":"Sommer","sequence":"additional","affiliation":[{"name":"Present address: Department of Biochemistry and Biophysics, UC San Francisco, San Francisco, CA 94143, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042605014181900_AVERY-AND-HORVITZ-1987","unstructured":"Avery, L. and Horvitz, H. R. (1987). A cell that dies during wild-type C. elegans development can function as a neuron in a ced-3 mutant. Cell51, 1071-1078."},{"key":"2021042605014181900_BRANDA-AND-STERN-2000","doi-asserted-by":"crossref","unstructured":"Branda, C. S. and Stern, M. J. (2000). Mechanisms controlling sex myoblast migration in Caenorhabditis elegans hermaphrodites. Dev. Biol.226, 137-151.","DOI":"10.1006\/dbio.2000.9853"},{"key":"2021042605014181900_BURDINE-ETAL-1998","doi-asserted-by":"crossref","unstructured":"Burdine, R. D., Branda, C. S. and Stern, M. J. (1998). EGL-17(FGF) expression coordinates the attraction of the migrating sex myoblasts with vulval induction in C.elegans. Development125, 1083-1093.","DOI":"10.1242\/dev.125.6.1083"},{"key":"2021042605014181900_CHANG-ETAL-1999","unstructured":"Chang, C., Newman, A. P. and Sternberg, P. W. (1999). Reciprocal EGF signaling back to the uterus from the induced C. elegans vulva coordinates morphogenesis of epithelia. Curr. Biol.9, 237-246."},{"key":"2021042605014181900_CORSI-ETAL-2000","doi-asserted-by":"crossref","unstructured":"Corsi, A. K., Kostas, S. A., Fire, A. and Krause, M. (2000). Caenorhabditis elegans twist plays an essential role in non-striated muscle development. Development127, 2041-2051.","DOI":"10.1242\/dev.127.10.2041"},{"key":"2021042605014181900_COSTA-ETAL-1998","unstructured":"Costa, M., Weir, M., Coulson, A., Sulston, J. and Kenyon, C. (1998). Posterior pattern formation in C. elegans involves posterior specific expression of a gene containing a homeobox. Cell55, 747-756."},{"key":"2021042605014181900_DEVORE-ETAL-1995","doi-asserted-by":"crossref","unstructured":"DeVore, D. L., Horvitz, H. R. and Stern, M. (1995). An FGF receptor signaling pathway is required for the normal cell migrations of the sex myoblasts in C.elegans hermaphrodites. 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A gonad-derived survival signal for vulval precursor cells in two nematode species. Curr. Biol.8, 287-290.","DOI":"10.1016\/S0960-9822(98)70111-3"},{"key":"2021042605014181900_FEACUTELIX-ETAL-2000","doi-asserted-by":"crossref","unstructured":"Fe\u0301lix, M. A., De Ley, P., Sommer, R. J., Frisse, L., Nadler, S. A., Thomas, K., Vanfleteren, J. and Sternberg, P. W. (2000). Evolution of vulva development in the Cephalobina (Nematoda). Dev. Biol.221, 68-86.","DOI":"10.1006\/dbio.2000.9665"},{"key":"2021042605014181900_GREENWALD-1997","unstructured":"Greenwald, I. (1997). Development of the vulva. In C. elegans II (ed. D. L. Riddle, T. Blumenthal, B. J. Meyer and J. R. Priess), pp. 519-542. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press."},{"key":"2021042605014181900_HARFE-ETAL-1998","doi-asserted-by":"crossref","unstructured":"Harfe, B. D., Gomes, A. V., Kenyon, C., Liu, J., Krause, M. and Fire, A. (1998). Analysis of a Caenorhabditis elegans twist homolog identifies conserved and divergent aspects of mesodermal patterning. Genes Dev. 12, 2623-2635.","DOI":"10.1101\/gad.12.16.2623"},{"key":"2021042605014181900_HILL-AND-STERNBERG-1992","doi-asserted-by":"crossref","unstructured":"Hill, R. J. and Sternberg, P. W. (1992). The gene lin-3 encodes an inductive signal for vulval development in C. elegans. Nature358, 470-476.","DOI":"10.1038\/358470a0"},{"key":"2021042605014181900_JUNGBLUT-AND-SOMMER-1998","doi-asserted-by":"crossref","unstructured":"Jungblut, B. and Sommer, R. J. (1998). The Pristionchus pacificus mab-5 gene is involved in the regulation of ventral epidermal cell fates. Curr. Biol. 8, 775-778.","DOI":"10.1016\/S0960-9822(98)70301-X"},{"key":"2021042605014181900_JUNGBLUT-AND-SOMMER-2000","doi-asserted-by":"crossref","unstructured":"Jungblut, B. and Sommer, R. J. (2000). Novel cell-cell interactions during vulva development in Pristionchus pacificus. 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Cell62, 1041-1052.","DOI":"10.1016\/0092-8674(90)90382-O"}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/128\/18\/3395\/1136524\/3395.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/128\/18\/3395\/1136524\/3395.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,25]],"date-time":"2022-12-25T07:04:21Z","timestamp":1671951861000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/128\/18\/3395\/41397\/Formation-of-the-egg-laying-system-in-Pristionchus"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,9,15]]},"references-count":40,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2001,9,15]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.128.18.3395","relation":{},"ISSN":["1477-9129","0950-1991"],"issn-type":[{"value":"1477-9129","type":"electronic"},{"value":"0950-1991","type":"print"}],"subject":[],"published":{"date-parts":[[2001,9,15]]}}}