{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T16:16:43Z","timestamp":1789661803209,"version":"4.0.1"},"reference-count":32,"publisher":"The Company of Biologists","issue":"2","license":[{"start":{"date-parts":[[2001,1,15]],"date-time":"2001-01-15T00:00:00Z","timestamp":979516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,1,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>In free-living nematodes, developmental processes like the formation of the vulva, can be studied at a cellular level. Cell lineage and ablation studies have been carried out in various nematode species and multiple changes in vulval patterning have been identified. In Pristionchus pacificus, vulva formation differs from Caenorhabditis elegans with respect to several autonomous and conditional aspects of cell fate specification. To understand the molecular basis of these evolutionary changes, we have performed a genetic analysis of vulva formation in P. pacificus. Here, we describe two mutants where the vulva is shifted posteriorly, affecting which precursor cells will form vulval tissue in P. pacificus. Mutant animals show a concomitant posterior displacement of the gonadal anchor cell, indicating that the gonad and the vulva are affected in a similar way. We show that mutations in the even-skipped homolog of nematodes, vab-7, cause these posterior displacements. In addition, cell ablation studies in the vab-7 mutant indicate that the altered position of the gonad not only changes the cell fate pattern but also the developmental competence of vulval precursor cells. Investigation of Cel-vab-7 mutant animals showed a similar but weaker vulva defective phenotype to the one described for Ppa-vab-7.<\/jats:p>","DOI":"10.1242\/dev.128.2.253","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T02:01:41Z","timestamp":1619402501000},"page":"253-261","source":"Crossref","is-referenced-by-count":13,"title":["The nematode\n                    <i>even-skipped<\/i>\n                    homolog\n                    <i>vab-7<\/i>\n                    regulates gonad and vulva position in\n                    <i>Pristionchus pacificus<\/i>"],"prefix":"10.1242","volume":"128","author":[{"given":"Benno","family":"Jungblut","sequence":"first","affiliation":[{"name":"Max-Planck Institut f\u00fcr Entwicklungsbiologie, Abt. Evolutionsbiologie, Spemannstrasse 37-39, 72076 T\u00fcbingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf J.","family":"Sommer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2001,1,15]]},"reference":[{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"1120","DOI":"10.1101\/gad.10.9.1120","article-title":"Posterior patterning by the Caenorhabditis even-skipped homolog vab-7","volume":"10","author":"Ahringer","year":"1996","journal-title":"Gen. Dev"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"3865","DOI":"10.1242\/dev.124.19.3865","article-title":"Maternal control of a zygotic patterning gene in Caenorhabditis elegans","volume":"124","author":"Ahringer","year":"1997","journal-title":"Development"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"1071","DOI":"10.1016\/0092-8674(87)90593-9","article-title":"A cell that dies during wild-type C. elegans development can function as a neuron in a ced-3 mutant","volume":"51","author":"Avery","year":"1987","journal-title":"Cell"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1006\/dbio.1996.8471","article-title":"Caenorhabditis elegans HOM-C genes regulate the response of vulval precursor cells to inductive signal","volume":"182","author":"Clandinin","year":"1997","journal-title":"Dev. Biol"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1242\/dev.1994.Supplement.143","article-title":"Developmental expression of the mouse Evx-2 gene: relationship with the evolution of the Hom\/Hox complex","volume":"120","author":"Doll\u00e9","year":"1994","journal-title":"Development"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"3667","DOI":"10.1242\/dev.125.18.3667","article-title":"The \u03b2-catenin homolog BAR-1 and LET-60 Ras coordinately regulate the Hox gene lin-39 during Caenorhabditis elegans vulva development","volume":"125","author":"Eisenmann","year":"1998","journal-title":"Development"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"452","DOI":"10.1126\/science.278.5337.452","article-title":"The homeotic gene lin-39 and the evolution and nematode epidermal cell fates","volume":"278","author":"Eizinger","year":"1997","journal-title":"Science"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/S0168-9525(99)01728-X","article-title":"Evolutionary change in the functional specificity of genes","volume":"15","author":"Eizinger","year":"1999","journal-title":"Trends Genet"},{"key":"2023022722551355800_","volume-title":"Methods in Cell Biology. Caenorhabditis elegans: Modern Biological Analysis of an Organism","author":"Epstein","year":"1995"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1002\/(SICI)1097-010X(19990415)285:1&lt;3::AID-JEZ2&gt;3.0.CO;2-J","article-title":"Evolution of developmental mechanisms in nematodes","volume":"285","author":"F\u00e9lix","year":"1999","journal-title":"J. Exp. Zool. (Dev. Mol. Evol.)"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"1617","DOI":"10.1098\/rspb.1999.0823","article-title":"Pristionchus pacificus, a nematode with only three juvenile stages, displays major heterochronic changes relative to Caenorhabditis elegans","volume":"266","author":"F\u00e9lix","year":"1999","journal-title":"Proc. R. Soc. London Ser. B"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"895","DOI":"10.1016\/0092-8674(90)90493-X","article-title":"The unc-86 gene product couples cell lineage and cell identity in C. elegans","volume":"63","author":"Finney","year":"1990","journal-title":"Cell"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"S40","DOI":"10.1101\/gr.4.1.S40","article-title":"On beyond classic RACE (rapid amplification of cDNA ends","volume":"4","author":"Frohmann","year":"1994","journal-title":"PCR Methods Appl"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"470","DOI":"10.1038\/358470a0","article-title":"The gene lin-3 encodes an inductive signal for vulval development in C. elegans","volume":"358","author":"Hill","year":"1992","journal-title":"Nature"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"775","DOI":"10.1016\/S0960-9822(98)70301-X","article-title":"The Pristionchus pacificus mab-5 gene is involved in the regulation of ventral epidermal cell fates","volume":"8","author":"Jungblut","year":"1998","journal-title":"Curr. Biol"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"3295","DOI":"10.1242\/dev.127.15.3295","article-title":"Novel cell-cell interactions during vulva development in Pristionchus pacificus","volume":"127","author":"Jungblut","year":"2000","journal-title":"Development"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"286","DOI":"10.1016\/0012-1606(81)90152-4","article-title":"Lineage alterations after ablation of cells of the somatic gonad of Caenorhabditis elegans","volume":"87","author":"Kimble","year":"1981","journal-title":"Dev. Biol"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1016\/S0168-9525(97)01005-6","article-title":"Vulva development in Caenorhabditis elegans","volume":"13","author":"Kornfeld","year":"1997","journal-title":"Trends Genet"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"981","DOI":"10.1016\/S0092-8674(00)81722-5","article-title":"lin-35 and lin-53, two genes that antagonize a C. elegans Ras pathway, encode proteins similar to Rb and its binding protein RbAp48","volume":"95","author":"Lu","year":"1998","journal-title":"Cell"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"621","DOI":"10.1093\/genetics\/120.3.621","article-title":"Genetic application of an inverse polymerase chain reaction","volume":"120","author":"Ochman","year":"1988","journal-title":"Genetics"},{"key":"2023022722551355800_","article-title":"A simple method to obtain the5\u2032 ends of mRNA sequences by direct ligation of CDNA-RNA hybrids to a plasmid vector","volume-title":"Tech. Tips Online","author":"Ranasinghe","year":"1998"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1007\/s004270050278","article-title":"Vulva formation in Pristionchus pacificus relies on continuous gonadal induction","volume":"209","author":"Sigrist","year":"1999","journal-title":"Dev. Genes Evol"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1038\/375142a0","article-title":"Sequential signaling during Caenorhabditis elegans vulval induction","volume":"375","author":"Simske","year":"1995","journal-title":"Nature"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1002\/bies.950190308","article-title":"Evolution and development \u2013 the nematode vulva as a case study","volume":"19","author":"Sommer","year":"1997","journal-title":"BioEssays"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"443","DOI":"10.1016\/S0959-437X(00)00110-6","article-title":"Evolution of nematode development","volume":"10","author":"Sommer","year":"2000","journal-title":"Curr. Opin. Genet. Dev"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1006\/dbio.1995.1007","article-title":"Evolution of cell lineage and pattern formation in the vulval equivalence group of rhabditid nematodes","volume":"167","author":"Sommer","year":"1995","journal-title":"Dev. Biol"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"52","DOI":"10.1016\/S0960-9822(02)00421-9","article-title":"Apoptosis and change of competence limit the size of the vulva equivalence group in Pristionchus pacificus: a genetic analysis","volume":"6","author":"Sommer","year":"1996","journal-title":"Curr. Biol"},{"key":"2023022722551355800_","first-page":"511","article-title":"Morphological, genetic and molecular description of Pristionchus pacificus sp. n. (Nematoda: Neodiplogastridae","volume":"19","author":"Sommer","year":"1996","journal-title":"Fund. Appl. Nemat"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"761","DOI":"10.1016\/0092-8674(86)90842-1","article-title":"Pattern formation during vulval development in C. elegans","volume":"44","author":"Sternberg","year":"1986","journal-title":"Cell"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"110","DOI":"10.1016\/0012-1606(77)90158-0","article-title":"Postembryonic cell lineages of the nematode Caenorhabditis elegans","volume":"56","author":"Sulston","year":"1977","journal-title":"Dev. Biol"},{"key":"2023022722551355800_","doi-asserted-by":"publisher","first-page":"1041","DOI":"10.1016\/0092-8674(90)90382-O","article-title":"Cell interactions coordinate the development of the C. elegans egg-laying system","volume":"62","author":"Thomas","year":"1990","journal-title":"Cell"},{"key":"2023022722551355800_","author":"Wood","year":"1988"}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/128\/2\/253\/2542140\/develop_128_2_253.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/128\/2\/253\/2542140\/develop_128_2_253.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,2,27]],"date-time":"2023-02-27T17:55:17Z","timestamp":1677520517000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/128\/2\/253\/41405\/The-nematode-even-skipped-homolog-vab-7-regulates"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,1,15]]},"references-count":32,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2001,1,15]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.128.2.253","relation":{},"ISSN":["0950-1991","1477-9129"],"issn-type":[{"value":"0950-1991","type":"print"},{"value":"1477-9129","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,1,15]]}}}