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We show that one of the five isoforms of C. elegans eIF4E, IFE-1, is enriched in the germline and is a component of germ granules (P granules). The association of IFE-1 with P granules requires the P-granule protein PGL-1. In vitro PGL-1 interacts directly with IFE-1, but not with the other four isoforms of eIF4E. Analysis of animals depleted of IFE-1 by RNAi shows that IFE-1 is required for spermatogenesis, specifically for efficient progression through the meiotic divisions and for the production of functional sperm, in both hermaphrodites and males. The requirement for IFE-1 is highly sensitive to temperature. IFE-1 is not required for oogenesis, as ife-1(RNAi) hermaphrodites produce viable progeny when normal sperm are supplied. Consistent with a primary role in spermatogenesis, ife-1 mRNA levels are highest in regions of the gonad undergoing spermatogenesis. Our results suggest that C. elegans spermatogenesis requires either this specific isoform of eIF4E or an elevated level of eIF4E.<\/jats:p>","DOI":"10.1242\/dev.128.20.3899","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T04:36:27Z","timestamp":1619411787000},"page":"3899-3912","source":"Crossref","is-referenced-by-count":106,"title":["An isoform of eIF4E is a component of germ granules and is required for spermatogenesis in<i>C. elegans<\/i>"],"prefix":"10.1242","volume":"128","author":[{"given":"Anahita","family":"Amiri","sequence":"first","affiliation":[{"name":"Department of Biology, Jordan Hall, 1001 E. 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