{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T06:49:17Z","timestamp":1790232557188,"version":"4.1.0"},"reference-count":54,"publisher":"The Company of Biologists","issue":"24","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,12,15]]},"abstract":"<jats:p>Phospholipase C-\u03b31 (PLC-\u03b31) is involved in a variety of intracellular signaling via many growth factor receptors and T-cell receptor. To explore the role of PLC-\u03b31 in vivo, we generated the PLC-\u03b31-deficient (plc-\u03b31\u2013\/\u2013) mice, which died of growth retardation at embryonic day 8.5-9.5 in utero. Therefore, we examined plc-\u03b31\u2013\/\u2013 chimeric mice generated with plc-\u03b31\u2013\/\u2013 embryonic stem (ES) cells for further study. Pathologically, plc-\u03b31\u2013\/\u2013 chimeras showed multicystic kidney due to severe renal dysplasia and renal tube dilation. Flow cytometric analysis and glucose phosphate isomerase assay revealed very few hematopoietic cells derived from the plc-\u03b31\u2013\/\u2013 ES cells in the mutant chimeras. However, differentiation of plc-\u03b31\u2013\/\u2013 ES cells into erythrocytes and monocytes\/macrophages in vitro was observed to a lesser extent compared with control wild-type ES cells. These data suggest that PLC-\u03b31 plays an essential role in the renal development and hematopoiesis in vivo.<\/jats:p>","DOI":"10.1242\/dev.128.24.5173","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T04:45:19Z","timestamp":1619412319000},"page":"5173-5180","source":"Crossref","is-referenced-by-count":38,"title":["Deficiency of phospholipase C-\u03b31 impairs renal development and hematopoiesis"],"prefix":"10.1242","volume":"128","author":[{"given":"Masatoshi","family":"Shirane","sequence":"first","affiliation":[{"name":"Department of Product Research and"},{"name":"Department of Molecular Oncology, Nippon Roche Research Center, Kajiwara 200, Kamakura, Kanagawa 247-8530, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirofumi","family":"Sawa","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Cellular Pathology, Hokkaido University Graduate School of Medicine, CREST, JST, N15W7, Kita-Ku, Sapporo 060-8638, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshiyasu","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Cellular Pathology, Hokkaido University Graduate School of Medicine, CREST, JST, N15W7, Kita-Ku, Sapporo 060-8638, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toru","family":"Nakano","sequence":"additional","affiliation":[{"name":"Department of Molecular Cell Biology, Osaka University Research Institute for Microbial Diseases, Yamadaoka 3-1, Suita, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Kitajima","sequence":"additional","affiliation":[{"name":"Department of Molecular Cell Biology, Osaka University Research Institute for Microbial Diseases, Yamadaoka 3-1, Suita, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoichi","family":"Shinkai","sequence":"additional","affiliation":[{"name":"Department of Molecular Oncology, Nippon Roche Research Center, Kajiwara 200, Kamakura, Kanagawa 247-8530, Japan"},{"name":"Department of Cell Biology, Institute for Virus Research, Kyoto University, Seigoinkawara-machi 53, Sakyo-Ku, Kyoto 606-8507, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuo","family":"Nagashima","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular and Cellular Pathology, Hokkaido University Graduate School of Medicine, CREST, JST, N15W7, Kita-Ku, Sapporo 060-8638, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Izumi","family":"Negishi","sequence":"additional","affiliation":[{"name":"Department of Molecular Oncology, Nippon Roche Research Center, Kajiwara 200, Kamakura, Kanagawa 247-8530, Japan"},{"name":"Department of Dermatology, Gunma University School of Medicine, Showa-machi 3-39-22, Maebashi, Gunma 371-8511, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042604125735200_BERGER-ETAL-1995","doi-asserted-by":"crossref","unstructured":"Berger, C. N., Tam, P. P. L. and Strum, K. S. (1995). The development haematopoietic cells is based in embryonic stem cell chimeras. Dev. Biol.170, 651\u2013663.","DOI":"10.1006\/dbio.1995.1244"},{"key":"2021042604125735200_BOUDOT-ETAL-1999","doi-asserted-by":"crossref","unstructured":"Boudot, C., Petitfrere, E., Kadri, Z., Chretien, S., Mayeux, P., Haye, B. and Billat, C. (1999). Erythropoietin induces glycosylphosphatidylinositol hydrolysis. Possible involvement of phospholipase c-\u03b32. J. Biol. Chem.274, 33966-33972.","DOI":"10.1074\/jbc.274.48.33966"},{"key":"2021042604125735200_BOURETTE-ETAL-1997","doi-asserted-by":"crossref","unstructured":"Bourette, R. P., Myles, G. M., Choi, J. L. and Rohrschneider, L. R. (1997). Sequential activation of phosphatidylinositol 3-kinase and phospholipase C-\u03b32 by the M-CSF receptor is necessary for differentiation signaling. EMBO J.16, 5880-5893.","DOI":"10.1093\/emboj\/16.19.5880"},{"key":"2021042604125735200_BRADLEY-1987","unstructured":"Bradley, A. (1987). Teratocarcinoma and Embryonic Stem Cells: A Practical Approach, pp.113-152. Oxford: IRL Press."},{"key":"2021042604125735200_CANTRELL-1996","doi-asserted-by":"crossref","unstructured":"Cantrell, D. (1996). T cell antigen receptor signal transduction pathways. Ann. Rev. Immunol.14, 259-274.","DOI":"10.1146\/annurev.immunol.14.1.259"},{"key":"2021042604125735200_COWLEY-AND-RUPP-1995","doi-asserted-by":"crossref","unstructured":"Cowley, B. D. and Rupp, J. C. (1995). Abnormal expression of epidermal growth factor and sulfated glycoprotein SGP-2 messenger RNA in a rat model of autosomal dominant polycystic kidney disease. J. Am. Soc. Nephrol.6, 1679-1681.","DOI":"10.1681\/ASN.V661679"},{"key":"2021042604125735200_CROSBY-ETAL-1998","doi-asserted-by":"crossref","unstructured":"Crosby, J. R., Seifert, R. A., Soriano, P. and Bowen-Pope, D. F. (1998). Chimaeric analysis reveals role of Pdgf receptors in all muscle lineages. Nature Genet.18, 385-388.","DOI":"10.1038\/ng0498-385"},{"key":"2021042604125735200_CYBULSKY-ETAL-1994","doi-asserted-by":"crossref","unstructured":"Cybulsky, A. V., Goodyer, P. R. and McTavish, A. J. (1994). Epidermal growth factor receptor activation in developing rat kidney. Am. J. Physiol.267, 428-436.","DOI":"10.1152\/ajprenal.1994.267.3.F428"},{"key":"2021042604125735200_DOETSCHMAN-ETAL-1985","doi-asserted-by":"crossref","unstructured":"Doetschman, T. C., Eistetter, H., Katz, M., Schmidt, W. and Kemler, R. (1985). The in vitro development of blastocyst-derived embryonic stem cell lines: formation of visceral yolk sac, blood islands and myocardium. J. Embryol. Exp. Morphol.87, 27-45.","DOI":"10.1242\/dev.87.1.27"},{"key":"2021042604125735200_EL-DAHR-ETAL-2000","doi-asserted-by":"crossref","unstructured":"El-Dahr, S. S., Harrison-Bernard, L. M., Dipp, S., Yosipiv, I. V. and Meleg-Smith, S. (2000). Bradykinin B2 null mice are prone to renal dysplasia: gene-environment interactions in kidney development. Physiol. Genomics3, 121-131.","DOI":"10.1152\/physiolgenomics.2000.3.3.121"},{"key":"2021042604125735200_GATTONE-ETAL-1990","doi-asserted-by":"crossref","unstructured":"Gattone, V. H., Andrews, G. K., Niu, F. W., Chadwick, L. J., Klein, R. M. and Calvet, J. P. (1990). Defective epidermal growth factor gene expression in mice with polycystic kidney disease. Dev. Biol. 138, 225-230.","DOI":"10.1016\/0012-1606(90)90192-L"},{"key":"2021042604125735200_GATTONE-ETAL-1995","doi-asserted-by":"crossref","unstructured":"Gattone, V. H., Lowden, D. A. and Cowley, B. D. (1995). Epidermal growth factor ameliorates autosomal recessive polycystic kidney disease in mice. Dev. Biol.169,504-510.","DOI":"10.1006\/dbio.1995.1164"},{"key":"2021042604125735200_GROENEN-ETAL-1996","doi-asserted-by":"crossref","unstructured":"Groenen, P. M. A., Garcia, E., Debber, P., Devriendt, K., Fryns, J. P. and Van de Ven, W. J. M. (1996). Structure, sequence, and chromosome 19 location of human USF2 and its rearrangement in a patient with multicystic renal dysplasia. Genomics38, 141-148.","DOI":"10.1006\/geno.1996.0609"},{"key":"2021042604125735200_GROUNDS-ETAL-1991","unstructured":"Grounds, M. D., Lai, M. C., Fan, Y., Codling, J. C. and Beilharz, M. W. (1991). Transplantation in the mouse model \u2013the use of a Y-chromosome-specific DNA clone to identify donor cells in situ. Transplantation52, 1101-1105."},{"key":"2021042604125735200_HOMMA-ETAL-1989","doi-asserted-by":"crossref","unstructured":"Homma, Y., Takenawa, T., Emori, Y., Sorimachi, H. and Suzuki, K. (1989). Tissue- and cell type-specific expression of mRNA for four types of inositol phospholipid specific phospholipase C. Biochem. Biophys. Res. Commun.164, 406-412.","DOI":"10.1016\/0006-291X(89)91734-8"},{"key":"2021042604125735200_HORIKOSHI-ETAL-1991","doi-asserted-by":"crossref","unstructured":"Horikoshi, S., Kubota, S., Martin, G. R., Yamada, Y. and Klotman, P. E. (1991). Epidermal growth factor (EGF) expression in the congenital polycystic mouse kidney. Kidney Int.39, 57-62.","DOI":"10.1038\/ki.1991.7"},{"key":"2021042604125735200_JI-ETAL-1997","doi-asserted-by":"crossref","unstructured":"Ji, Q.-S., Winner, G. E., Niswender, K. D., Horstman, D., Wisdom, R., Magnuson, M. A. and Carpenter, G. (1997). Essential role of the tyrosine kinase substrate phospholipase C-\u03b31 in mammalian growth and development. Proc. Natl. Acad. Sci. USA94, 2999-3003.","DOI":"10.1073\/pnas.94.7.2999"},{"key":"2021042604125735200_LEVEACUTEEN-ETAL-1994","doi-asserted-by":"crossref","unstructured":"Leve\u0301en, P., Pekny, M., Gebre-Medhin, S., Swolin, B., Larsson, E. and Betsholtz, C. (1994).Mice deficient for PDGF B show renal, cardiovascular and hematologocal abnormalities. Genes Dev.8, 1875-1887.","DOI":"10.1101\/gad.8.16.1875"},{"key":"2021042604125735200_LINDAHL-ETAL-1998","doi-asserted-by":"crossref","unstructured":"Lindahl, P., Hellstrom, M., Kalen, M., Karlsson, L., Pekny, M., Pekna, M., Soriano, P. and Betsholtz, C. (1998). Paracrine PDGF-B\/PDGF-R\u03b2 signaling controls mesangial cell development in kidney glomeruli. Development125, 3313-3322.","DOI":"10.1242\/dev.125.17.3313"},{"key":"2021042604125735200_LOPEZ-ETAL-2000","unstructured":"Lopez, I., Mak, E. C., Ding, J., Hamm, H. E. and Lomasney, J. W. (2000). A novel bifunctional phospholipase C that is regulated by G\u03b112 and stimulates the Ras\/MAP kinase pathway. J. Biol. Chem.276, 2758-2765."},{"key":"2021042604125735200_LYON-AND-SEARLE-1990","unstructured":"Lyon, M. F. and Searle, A. G. (1990). Genetic Variants and Strains of the Laboratory Mouse. Oxford: Oxford University Press."},{"key":"2021042604125735200_MAJERUS-1992","doi-asserted-by":"crossref","unstructured":"Majerus, P. W. (1992). Inositol phosphate biochemistry. Ann. Rev. Biochem.61, 225-250.","DOI":"10.1146\/annurev.bi.61.070192.001301"},{"key":"2021042604125735200_MOHAMMADI-ETAL-1992","doi-asserted-by":"crossref","unstructured":"Mohammadi, M., Dionne, C. A., Li, W., Li, N., Spivak, T., Honegger, A. M., Jaye, M. and Schlessinger, J. (1992). Point mutation in FGF receptor eliminates phosphatidylinositol hydrolysis without affecting mitogenesis. Nature358, 681-684.","DOI":"10.1038\/358681a0"},{"key":"2021042604125735200_MOORE-ETAL-1996","doi-asserted-by":"crossref","unstructured":"Moore, M. W., Klein, R. D., Farinas, I., Sauer, H., Armanini, M., Phillips, H., Reichardt, L. F., Ryan, A. M., Carver-Moore, K. and Rosenthal, A. (1996). Renal and neuronal abnormalities in mice lacking GDNF. Nature382, 76-79.","DOI":"10.1038\/382076a0"},{"key":"2021042604125735200_MORRISON-GRAHAM-AND-TAKAHASHI-1993","doi-asserted-by":"crossref","unstructured":"Morrison-Graham, K. and Takahashi, Y. (1993). Steel factor and c-kit receptor: From mutants to a growth factor system. BioEssays15, 77-83.","DOI":"10.1002\/bies.950150202"},{"key":"2021042604125735200_NAKAMURA-ETAL-1993","doi-asserted-by":"crossref","unstructured":"Nakamura, T., Ebihara, I., Nagaoka, I., Tomino, Y., Nagao, S., Takahashi, H. and Koide, H. (1993). Growth factor gene expression in kidney of murine polycystic kidney disease. J. Am. Soc. Nephrol.3, 1378-1386.","DOI":"10.1681\/ASN.V371378"},{"key":"2021042604125735200_NAKANO-ETAL-1994","doi-asserted-by":"crossref","unstructured":"Nakano, T., Kodama, H. and Honjo, T. (1994). Generation of lymphohematopoietic cells from embryonic stem cells in culture. Science265, 1098-1101.","DOI":"10.1126\/science.8066449"},{"key":"2021042604125735200_NAKAYAMA-ETAL-1993","doi-asserted-by":"crossref","unstructured":"Nakayama, K-i., Nakayama, K., Negishi, I., Kuida, K., Shinkai, Y., Louie, M. C., Fields, L. E., Lucas, P. J., Stewart, V., Alt, F. and Loh, D. Y. (1993). Disappearance of the lymphoid system in bcl-2 homozygous mutant chimeric mice. Science261, 1584-1588.","DOI":"10.1126\/science.8372353"},{"key":"2021042604125735200_NEBIGIL-1997","doi-asserted-by":"crossref","unstructured":"Nebigil, C. G. (1997). Suppression of phospholipase C beta, gamma, and delta families alters cell growth and phosphatidylinositol 4,5-bisphosphate levels. Biochemistry36, 15949-15958.","DOI":"10.1021\/bi971721m"},{"key":"2021042604125735200_PERANTONI-ETAL-1995","doi-asserted-by":"crossref","unstructured":"Perantoni, A. O., Dove, L. F. and Karavanova, I. (1995). Basic fibroblast growth factor can mediate the early inductive events in renal development. Proc. Natl. Acad. Sci. USA92, 4696-4700.","DOI":"10.1073\/pnas.92.10.4696"},{"key":"2021042604125735200_PETERS-ETAL-1992","doi-asserted-by":"crossref","unstructured":"Peters, K. G., Marie, J., Wilson, E., Ives, H. E., Escobedo, J., Del Rosario, M., Mirda, D. and Williams, L. T. (1992). Point mutation of an FGF receptor abolishes phosphatidylinositol turnover and Ca2+ flux but not mitogenesis. Nature358, 678-681.","DOI":"10.1038\/358678a0"},{"key":"2021042604125735200_PEVNY-ETAL-1991","doi-asserted-by":"crossref","unstructured":"Pevny, L., Simon, M. C., Robertson, E., Klein, W. H., Tsai. S.-F., D\u2019Agati, V., Orkin, S. H. and Constantini, F. (1991). Erythroid differentiation in chimeric mice blocked by a targeted mutation in the gene for transcription factor GATA-1. Nature349, 257-260.","DOI":"10.1038\/349257a0"},{"key":"2021042604125735200_PICHEL-ETAL-1996","doi-asserted-by":"crossref","unstructured":"Pichel, J. G., Shen, L., Sheng, H. Z., Granholm, A. C., Drago, J., Grinberg, A., Lee, E. J., Huang, S. P., Saarma, M., Hoffer, B. J., Sariola, H. and Westphal, H. (1996). Defects in enteric innervation and kidney development in mice lacking GDNF. Nature382, 73-76.","DOI":"10.1038\/382073a0"},{"key":"2021042604125735200_REN-ETAL-1994","doi-asserted-by":"crossref","unstructured":"Ren, H. Y., Komatsu, N., Shimizu, R., Okada, K. and Miura, Y. (1994). Erythropoietin induces tyrosine phosphorylation and activation of phospholipase C-gamma 1 in a human erythropoietin-dependent cell line. J. Biol. Chem.269, 19633-19638.","DOI":"10.1016\/S0021-9258(17)32216-0"},{"key":"2021042604125735200_RHEE-AND-CHOI-1992","doi-asserted-by":"crossref","unstructured":"Rhee, S. G. and Choi, K. D. (1992). Regulation of inositol phospholipid-specific phospholipase C isozymes. J. Biol. Chem.276, 12393-12396.","DOI":"10.1016\/S0021-9258(18)42284-3"},{"key":"2021042604125735200_ROCHE-ETAL-1996","doi-asserted-by":"crossref","unstructured":"Roche, S., McGlade, J., Jones, M., Gish, G. D., Pawson, T. and Courtneidge, S. A. (1996). Reqirment of phospholipase C\u03b3, the tyrosine phosphatase Syp and the adaptor proteins Shc and Nck for PDGF-induced DNA synthesis: evidence for the existence of Ras-dependent and Ras-independent pathways. EMBO J.15, 4940-4948.","DOI":"10.1002\/j.1460-2075.1996.tb00874.x"},{"key":"2021042604125735200_ROUMLNNSTRAND-ETAL-1992","doi-asserted-by":"crossref","unstructured":"Ro\u0308nnstrand, L., Mori, S., Arridsson, A.-K., Eriksson, A., Wernstedt, C., Hellman, U., Claesson-Welsh, L. and Heldin, C.-H. (1992). Identification of two C-terminal autophosphorylation sites in the PDGF\u03b2-receptor: involvement in the interaction with phospholipase C-\u03b3. EMBO J.11, 3911-3919.","DOI":"10.1002\/j.1460-2075.1992.tb05484.x"},{"key":"2021042604125735200_ROTHSCHILD-ETAL-1992","doi-asserted-by":"crossref","unstructured":"Rothschild, C. B., Akots, G., Fajans, S. S. and Bowden, D. W. (1992). A microsatellite polymorphism associated with the PLC1 (phospholipase C) locus: Identification, mapping, and linkage to the MODY locus on chromosome 20. Genomics13, 560-564.","DOI":"10.1016\/0888-7543(92)90125-C"},{"key":"2021042604125735200_SAACUTENCHEZ-ETAL-1996","doi-asserted-by":"crossref","unstructured":"Sa\u0301nchez, M. P., Silos-Santiago, I., Frisen, J., He, B., Lira, S. A. and Barbacid, M. (1996). Renal agenesis and the absence of enteric neurons in mice lacking GDNF. Nature382, 70-73.","DOI":"10.1038\/382070a0"},{"key":"2021042604125735200_SCHUCHARDT-ETAL-1996","doi-asserted-by":"crossref","unstructured":"Schuchardt, A., D\u2019Agati, V., Pachnis, V. and Costantini, F. (1996). Renal agenesis and hypodysplasia in ret-k\u2013 mutant mice result from defects in ureteric bud development. Development122, 1919-1929.","DOI":"10.1242\/dev.122.6.1919"},{"key":"2021042604125735200_SHALABY-ETAL-1995","doi-asserted-by":"crossref","unstructured":"Shalaby, F., Rossant, J., Yamaguchi, T. P., Gertsenstein, M., Wu, X.-F., Breitman, M. L. and Schuh, A. C. (1995). Failure of blood-island formation and vaculogenesis in Flk-1-deficient mice. Nature376, 62-66.","DOI":"10.1038\/376062a0"},{"key":"2021042604125735200_SONG-ETAL-2000","unstructured":"Song, C., Hu, C.-D., Masago, M., Kariya, Ki., Yamawaki-Kataoka, Y., Shibatohge, M., Wu, D., Satoh, T. and Kataoka, T. (2000). Regulation of a novel human phospholipase C, PLC-\u03b5, through membrane targeting by Ras. J. Biol. Chem.276, 2752-2757."},{"key":"2021042604125735200_SORIANO-1994","doi-asserted-by":"crossref","unstructured":"Soriano, P. (1994). Abnormal kidney development and hematological disorders in PDGF\u03b2-receptor mutant mice. Genes Dev.8, 1888-1896.","DOI":"10.1101\/gad.8.16.1888"},{"key":"2021042604125735200_THREADGILL-ETAL-1995","doi-asserted-by":"crossref","unstructured":"Threadgill, D. W., Dlugosz, A. A., Hansen, L. A., Tennenbaum, T., Lichti. U., Yee, D., LaMantia, C., Mourton, T., Herrup, K. and Harris, R. C. (1995). Targeted disruption of mouse EGF receptor: effect of genetic background on mutant phenotype. Science269, 230-234.","DOI":"10.1126\/science.7618084"},{"key":"2021042604125735200_TSAI-ETAL-1994","doi-asserted-by":"crossref","unstructured":"Tsai, F.-Y., Keller, G., Kuo, F. C., Weiss, M., Chem, J., Rosenblatt, M., Alt, F. W. and Orkin, S. H. (1994). An early haematopoietic defect in mice lacking the transcription factor GATA-2. Nature371, 221-226.","DOI":"10.1038\/371221a0"},{"key":"2021042604125735200_ULLRICH-AND-SCHLESSINGER-1990","doi-asserted-by":"crossref","unstructured":"Ullrich, A. and Schlessinger, J. (1990). Signal transduction by receptors with tyrosine kinase activity. Cell61, 203-212.","DOI":"10.1016\/0092-8674(90)90801-K"},{"key":"2021042604125735200_VAINIO-AND-MULLER-1997","doi-asserted-by":"crossref","unstructured":"Vainio, S. and Muller, U. (1997). Inductive tissue interactions, cell signaling, and the control of kidney organogenesis. Cell90, 975-978.","DOI":"10.1016\/S0092-8674(00)80363-3"},{"key":"2021042604125735200_VALIUS-AND-KAZLAUSKAS-1993","doi-asserted-by":"crossref","unstructured":"Valius, M. and Kazlauskas, A. (1993). Phospholipase C-gamma 1 and phosphatidylinositol 3 kinase are the downstream mediators of the PDGF receptor\u2019s mitogenic signal. Cell73, 321-334.","DOI":"10.1016\/0092-8674(93)90232-F"},{"key":"2021042604125735200_VENEMA-ETAL-1998","doi-asserted-by":"crossref","unstructured":"Venema, V. J., Ju, H., Sun, J., Eaton, D. C., Marrero, M. B. and Venema, R. C. (1998). Bradykinin stimulates the tyrosine phosphorylation and bradykinin B2 receptor association of phospholipase C gamma 1 in vascular endothelial cells. Biochem. Biophys. Res. Commun.246, 70-75.","DOI":"10.1006\/bbrc.1998.8574"},{"key":"2021042604125735200_WANG-ETAL-2000","doi-asserted-by":"crossref","unstructured":"Wang, D., Feng, J., Wen, R., Marine, J. C., Sangster, M. Y., Parganas, E., Hoffmeyer, A., Jackson, C. W., Cleveland, J. L., Murray, P. J. and Ihle, J. N. (2000). Phospholipase C\u03b32 is essential in the functions of B cell and several Fc receptors. Immunity13, 25-35.","DOI":"10.1016\/S1074-7613(00)00005-4"},{"key":"2021042604125735200_WEINSTEIN-ETAL-1999","unstructured":"Weinstein, T., Hwang, D., Lev-Ran, A., Ori, Y., Korzets, A. and Levi, J. (1999). Excretion of epidermal growth factor in human adult polycystic kidney disease. Isr. J. Med. Sci.33, 641-642."},{"key":"2021042604125735200_WEN-ETAL-1999","doi-asserted-by":"crossref","unstructured":"Wen, J. G., Fr\u00f8ki\u00e6r, J., J\u00f8rgensen, T. M. and Djurhuus, J. C. (1999). Obstructive nephropathy: an update of the experimental research. Urol. Res.27, 29-39.","DOI":"10.1007\/s002400050086"},{"key":"2021042604125735200_WINYARD-ETAL-1997","doi-asserted-by":"crossref","unstructured":"Winyard, P. J., Bao, Q., Hughes, R. C. and Woolf, A. S. (1997). Epithelial galectin-3 during human nephrogenesis and childhood cystic diseases. J. Am. Soc. Nephrol.8, 1647-1657.","DOI":"10.1681\/ASN.V8111647"},{"key":"2021042604125735200_XIE-AND-BIKLE-1999","doi-asserted-by":"crossref","unstructured":"Xie, Z. and Bikle, D. D. (1999). Phospholipase C-gamma1 is required for calcium-induced keratinocyte differentiation. J. Biol. Chem.274, 20421-20424.","DOI":"10.1074\/jbc.274.29.20421"}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/128\/24\/5173\/1138790\/5173.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/128\/24\/5173\/1138790\/5173.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,2]],"date-time":"2023-11-02T18:41:52Z","timestamp":1698950512000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/128\/24\/5173\/41584\/Deficiency-of-phospholipase-C-1-impairs-renal"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,12,15]]},"references-count":54,"journal-issue":{"issue":"24","published-print":{"date-parts":[[2001,12,15]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.128.24.5173","relation":{},"ISSN":["1477-9129","0950-1991"],"issn-type":[{"value":"1477-9129","type":"electronic"},{"value":"0950-1991","type":"print"}],"subject":[],"published":{"date-parts":[[2001,12,15]]}}}