{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T12:03:20Z","timestamp":1790942600648,"version":"4.1.0"},"reference-count":46,"publisher":"The Company of Biologists","issue":"4","license":[{"start":{"date-parts":[[2001,2,15]],"date-time":"2001-02-15T00:00:00Z","timestamp":982195200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Recent loss-of-function studies in mice show that the transcription factor GATA6 is important for visceral endoderm differentiation. It is also expressed in early bronchial epithelium and the observation that this tissue does not receive any contribution from Gata6 double mutant embryonic stem (ES) cells in chimeric mice suggests that GATA6 may play a crucial role in lung development. The aim of this study was to determine the role of GATA6 in fetal pulmonary development. We show that Gata6 mRNA is expressed predominantly in the developing pulmonary endoderm and epithelium, but at E15.5 also in the pulmonary mesenchyme. Blocking or depleting GATA6 function results in diminished branching morphogenesis both in vitro and in vivo. TTF1 expression is unaltered in chimeric lungs whereas SPC and CC10 expression are attenuated in abnormally branched areas of chimeric lungs. Chimeras generated in a ROSA26 background show that endodermal cells in these abnormally branched areas are derived from Gata6 mutant ES cells, implicating that the defect is intrinsic to the endoderm. Taken together, these data demonstrate that GATA6 is not essential for endoderm specification, but is required for normal branching morphogenesis and late epithelial cell differentiation.<\/jats:p>","DOI":"10.1242\/dev.128.4.503","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T04:46:17Z","timestamp":1619412377000},"page":"503-511","source":"Crossref","is-referenced-by-count":111,"title":["The transcription factor GATA6 is essential for branching morphogenesis and epithelial cell differentiation during fetal pulmonary development"],"prefix":"10.1242","volume":"128","author":[{"given":"Richard","family":"Keijzer","sequence":"first","affiliation":[{"name":"Erasmus University Medical Centre Rotterdam 1 Department of Cell Biology and Genetics , , Rotterdam, The Netherlands"},{"name":"Erasmus University Medical Centre Rotterdam 2 Department of Pediatric Surgery, Sophia Children\u2019s Hospital , , Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minke van","family":"Tuyl","sequence":"additional","affiliation":[{"name":"Erasmus University Medical Centre Rotterdam 2 Department of Pediatric Surgery, Sophia Children\u2019s Hospital , , Rotterdam, The Netherlands"},{"name":"Hospital for Sick Children Research Institute, Department of Pediatrics, University of Toronto 3 Lung Biology Research Program , , Toronto, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carel","family":"Meijers","sequence":"additional","affiliation":[{"name":"Erasmus University Medical Centre Rotterdam 1 Department of Cell Biology and Genetics , , Rotterdam, The Netherlands"},{"name":"Erasmus University Medical Centre Rotterdam 2 Department of Pediatric Surgery, Sophia Children\u2019s Hospital , , Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Post","sequence":"additional","affiliation":[{"name":"Hospital for Sick Children Research Institute, Department of Pediatrics, University of Toronto 3 Lung Biology Research Program , , Toronto, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dick","family":"Tibboel","sequence":"additional","affiliation":[{"name":"Erasmus University Medical Centre Rotterdam 2 Department of Pediatric Surgery, Sophia Children\u2019s Hospital , , Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Grosveld","sequence":"additional","affiliation":[{"name":"Erasmus University Medical Centre Rotterdam 1 Department of Cell Biology and Genetics , , Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manoussos","family":"Koutsourakis","sequence":"additional","affiliation":[{"name":"Erasmus University Medical Centre Rotterdam 1 Department of Cell Biology and Genetics , , Rotterdam, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2001,2,15]]},"reference":[{"key":"2023090514451897800_DEVELOP_128_4_503C1","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1016\/0092-8674(94)90522-3","article-title":"HNF-3\u03b2 is essential for node and notochord formation in mouse development","volume":"78","author":"Ang","year":"1994","journal-title":"Cell"},{"key":"2023090514451897800_DEVELOP_128_4_503C2","first-page":"2235","article-title":"Mouse GATA-4: a retinoic acid-inducible GATA-binding transcription factor expressed in endodermally derived tissues and heart","volume":"13","author":"Arceci","year":"1993","journal-title":"Mol. 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