{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T00:15:37Z","timestamp":1791591337890,"version":"4.3.4"},"reference-count":69,"publisher":"The Company of Biologists","issue":"6","license":[{"start":{"date-parts":[[2001,3,15]],"date-time":"2001-03-15T00:00:00Z","timestamp":984614400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2001,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The endothelial cell surface receptor thrombomodulin (TM) inhibits blood coagulation by forming a complex with thrombin, which then converts protein C into the natural anticoagulant, activated protein C. In mice, a loss of TM function causes embryonic lethality at day 8.5 p.c. (post coitum) before establishment of a functional cardiovascular system. At this developmental stage, TM is expressed in the developing vasculature of the embryo proper, as well as in non-endothelial cells of the early placenta, giant trophoblast and parietal endoderm. Here, we show that reconstitution of TM expression in extraembryonic tissue by aggregation of tetraploid wild-type embryos with TM-null embryonic stem cells rescues TM-null embryos from early lethality. TM-null tetraploid embryos develop normally during midgestation, but encounter a secondary developmental block between days 12.5 and 16.5 p.c. Embryos lacking TM develop lethal consumptive coagulopathy during this period, and no live embryos are retrieved at term. Morphogenesis of embryonic blood vessels and other organs appears normal before E15. These findings demonstrate a dual role of TM in development, and that a loss of TM function disrupts mouse embryogenesis at two different stages. These two functions of TM are exerted in two distinct tissues: expression of TM in non-endothelial extraembryonic tissues is required for proper function of the early placenta, while the absence of TM from embryonic blood vessel endothelium causes lethal consumptive coagulopathy.<\/jats:p>","DOI":"10.1242\/dev.128.6.827","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T04:40:26Z","timestamp":1619412026000},"page":"827-838","source":"Crossref","is-referenced-by-count":68,"title":["Tissue-restricted expression of thrombomodulin in the placenta rescues thrombomodulin-deficient mice from early lethality and reveals a secondary developmental block"],"prefix":"10.1242","volume":"128","author":[{"given":"Berend","family":"Isermann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara B.","family":"Hendrickson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristi","family":"Hutley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mark","family":"Wing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hartmut","family":"Weiler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2001,3,15]]},"reference":[{"key":"2023051200351529300_DEVELOP_128_6_827C1","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1038\/ng0595-119","article-title":"Targeted disruption of the mouse factor VIII gene produces a model of haemophilia A","volume":"10","author":"Bi","year":"1995","journal-title":"Nat.Genet"},{"key":"2023051200351529300_DEVELOP_128_6_827C2","doi-asserted-by":"publisher","first-page":"765","DOI":"10.1016\/S0015-0282(99)00360-X","article-title":"Thrombophilia-associated pregnancy wastage","volume":"72","author":"Blumenfeld","year":"1999","journal-title":"Fertil. Steril"},{"key":"2023051200351529300_DEVELOP_128_6_827C3","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1016\/S0268-960X(97)90013-8","article-title":"Thrombophilia and fetal loss","volume":"11","author":"Brenner","year":"1997","journal-title":"Blood Rev"},{"key":"2023051200351529300_DEVELOP_128_6_827C4","doi-asserted-by":"publisher","first-page":"69","DOI":"10.3109\/15419069609010764","article-title":"Expression patterns of adhesion receptors in the developing mouse lung: functional implications","volume":"4","author":"Buck","year":"1996","journal-title":"Cell Adhes.Commun"},{"key":"2023051200351529300_DEVELOP_128_6_827C5","doi-asserted-by":"publisher","first-page":"6258","DOI":"10.1073\/pnas.93.13.6258","article-title":"Fatal embryonic bleeding events in mice lacking tissue factor, the cell-associated initiator of blood coagulation","volume":"93","author":"Bugge","year":"1996","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023051200351529300_DEVELOP_128_6_827C6","doi-asserted-by":"publisher","first-page":"73","DOI":"10.1038\/383073a0","article-title":"Role of tissue factor in embryonic blood vessel development","volume":"383","author":"Carmeliet","year":"1996","journal-title":"Nature"},{"key":"2023051200351529300_DEVELOP_128_6_827C7","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1172\/JCI5678","article-title":"Factor VII deficiency rescues the intrauterine lethality in mice associated with a tissue factor pathway inhibitor deficit","volume":"103","author":"Chan","year":"1999","journal-title":"J. Clin. Invest"},{"key":"2023051200351529300_DEVELOP_128_6_827C8","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1172\/JCI7141","article-title":"A murine model of myocardial microvascular thrombosis","volume":"104","author":"Christie","year":"1999","journal-title":"J. Clin. Invest"},{"key":"2023051200351529300_DEVELOP_128_6_827C9","doi-asserted-by":"publisher","first-page":"1508","DOI":"10.1126\/science.7985020","article-title":"Implantation and the placenta: key pieces of the development puzzle","volume":"266","author":"Cross","year":"1994","journal-title":"Science"},{"key":"2023051200351529300_DEVELOP_128_6_827C10","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1038\/384066a0","article-title":"Fatal haemorrhage and incomplete block to embryogenesis in mice lacking coagulation factor V","volume":"384","author":"Cui","year":"1996","journal-title":"Nature"},{"key":"2023051200351529300_DEVELOP_128_6_827C11","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1055\/s-0037-1613783","article-title":"Blood coagulation factor X deficiency causes partial embryonic lethality and fatal neonatal bleeding in mice","volume":"83","author":"Dewerchin","year":"2000","journal-title":"Thromb. Haemost"},{"key":"2023051200351529300_DEVELOP_128_6_827C12","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1182\/blood.V75.2.329.329","article-title":"Structure and function of thrombomodulin: a natural anticoagulant","volume":"75","author":"Dittman","year":"1990","journal-title":"Blood"},{"key":"2023051200351529300_DEVELOP_128_6_827C13","doi-asserted-by":"publisher","first-page":"279","DOI":"10.1242\/dev.124.2.279","article-title":"Murine gastrulation requires HNF-4 regulated gene expression in the visceral endoderm: tetraploid rescue of Hnf-4(\u2212\/\u2212) embryos","volume":"124","author":"Duncan","year":"1997","journal-title":"Development"},{"key":"2023051200351529300_DEVELOP_128_6_827C14","doi-asserted-by":"publisher","first-page":"1348","DOI":"10.1126\/science.3029867","article-title":"The regulation of natural anticoagulant pathways","volume":"235","author":"Esmon","year":"1987","journal-title":"Science"},{"key":"2023051200351529300_DEVELOP_128_6_827C15","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1016\/S0167-4838(99)00266-6","article-title":"Regulation of blood coagulation","volume":"1477","author":"Esmon","year":"2000","journal-title":"Biochim. Biophys. Acta"},{"key":"2023051200351529300_DEVELOP_128_6_827C16","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1016\/S0143-4004(98)90057-1","article-title":"Increase in expression and activity of thrombomodulin in term human syncytiotrophoblast microvilli","volume":"19","author":"Fazel","year":"1998","journal-title":"Placenta"},{"key":"2023051200351529300_DEVELOP_128_6_827C17","doi-asserted-by":"publisher","first-page":"364","DOI":"10.1007\/BF00188735","article-title":"Thrombomodulin distribution during murine development","volume":"202","author":"Ford","year":"1993","journal-title":"Roux\u2019s Archiv. Dev. Biol"},{"key":"2023051200351529300_DEVELOP_128_6_827C18","doi-asserted-by":"publisher","first-page":"691","DOI":"10.1097\/00001721-199112000-00001","article-title":"The catalytic role of anionic phospholipids in the activation of protein C by factor Xa and expression of its anticoagulant function in human plasma","volume":"2","author":"Freyssinet","year":"1991","journal-title":"Blood Coagul. Fibrinolysis"},{"key":"2023051200351529300_DEVELOP_128_6_827C19","doi-asserted-by":"publisher","first-page":"518","DOI":"10.1038\/35006683","article-title":"Structural basis for the anticoagulant activity of the thrombin-thrombomodulin complex","volume":"404","author":"Fuentes-Prior","year":"2000","journal-title":"Nature"},{"key":"2023051200351529300_DEVELOP_128_6_827C20","doi-asserted-by":"publisher","first-page":"333","DOI":"10.1038\/371333a0","article-title":"Essential role of Mash-2 in extraembryonic development","volume":"371","author":"Guillemot","year":"1994","journal-title":"Nature"},{"key":"2023051200351529300_DEVELOP_128_6_827C21","doi-asserted-by":"publisher","first-page":"4188","DOI":"10.1182\/blood.V92.11.4188","article-title":"Intravascular coagulation activation in a murine model of thrombomodulin deficiency: effects of lesion size, age, and hypoxia on fibrin deposition","volume":"92","author":"Healy","year":"1998","journal-title":"Blood"},{"key":"2023051200351529300_DEVELOP_128_6_827C22","doi-asserted-by":"publisher","first-page":"850","DOI":"10.1073\/pnas.92.3.850","article-title":"Absence of the blood-clotting regulator thrombomodulin causes embryonic lethality in mice before development of a functional cardiovascular system","volume":"92","author":"Healy","year":"1995","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023051200351529300_DEVELOP_128_6_827C23","doi-asserted-by":"publisher","first-page":"944","DOI":"10.1182\/blood.V90.3.944","article-title":"Tissue factor pathway inhibitor gene disruption produces intrauterine lethality in mice","volume":"90","author":"Huang","year":"1997","journal-title":"Blood"},{"key":"2023051200351529300_DEVELOP_128_6_827C24","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1016\/0012-1606(87)90312-5","article-title":"Fetomodulin: marker surface protein of fetal development which is modulatable by cyclic AMP","volume":"122","author":"Imada","year":"1987","journal-title":"Dev. Biol"},{"key":"2023051200351529300_DEVELOP_128_6_827C25","doi-asserted-by":"publisher","first-page":"873","DOI":"10.1172\/JCI10489","article-title":"Complete antithrombin deficiency in mice results in embryonic lethality","volume":"106","author":"Ishiguro","year":"2000","journal-title":"J. Clin. Invest"},{"key":"2023051200351529300_DEVELOP_128_6_827C26","doi-asserted-by":"publisher","first-page":"1481","DOI":"10.1172\/JCI3011","article-title":"Inactivation of the gene for anticoagulant protein C causes lethal perinatal consumptive coagulopathy in mice","volume":"102","author":"Jalbert","year":"1998","journal-title":"J. Clin. Invest"},{"key":"2023051200351529300_DEVELOP_128_6_827C27","volume-title":"The Atlas of Mouse Development","author":"Kaufman","year":"1994"},{"key":"2023051200351529300_DEVELOP_128_6_827C28","first-page":"692","article-title":"Quantitation of a murine lung endothelial cell protein, P112, with a double monoclonal antibody assay","volume":"59","author":"Kennel","year":"1988","journal-title":"Lab. Invest"},{"key":"2023051200351529300_DEVELOP_128_6_827C29","doi-asserted-by":"publisher","first-page":"563","DOI":"10.1111\/j.1432-1033.1991.tb16051.x","article-title":"Different glycoforms of human thrombomodulin. Their glycosaminoglycan-dependent modulatory effects on thrombin inactivation by heparin cofactor II and antithrombin III","volume":"198","author":"Koyama","year":"1991","journal-title":"Eur. J. Biochem"},{"key":"2023051200351529300_DEVELOP_128_6_827C30","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1056\/NEJM199901073400102","article-title":"Increased frequency of genetic thrombophilia in women with complications of pregnancy","volume":"340","author":"Kupferminc","year":"1999","journal-title":"N. Engl. J. Med"},{"key":"2023051200351529300_DEVELOP_128_6_827C31","doi-asserted-by":"publisher","first-page":"489","DOI":"10.1242\/dev.121.2.489","article-title":"Defective development of the embryonic and extraembryonic circulatory systems in vascular cell adhesion molecule (VCAM-1) deficient mice","volume":"121","author":"Kwee","year":"1995","journal-title":"Development"},{"key":"2023051200351529300_DEVELOP_128_6_827C32","doi-asserted-by":"publisher","first-page":"1517","DOI":"10.1182\/blood.V95.5.1517.005k48_1517_1532","article-title":"Role of hemostatic gene polymorphisms in venous and arterial thrombotic disease","volume":"95","author":"Lane","year":"2000","journal-title":"Blood"},{"key":"2023051200351529300_DEVELOP_128_6_827C33","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1055\/s-0038-1650638","article-title":"Inherited thrombophilia: Part 1","volume":"76","author":"Lane","year":"1996","journal-title":"Thromb. Haemost"},{"key":"2023051200351529300_DEVELOP_128_6_827C34","doi-asserted-by":"publisher","first-page":"824","DOI":"10.1055\/s-0038-1650673","article-title":"Inherited thrombophilia: Part 2","volume":"76","author":"Lane","year":"1996","journal-title":"Thromb. Haemost"},{"key":"2023051200351529300_DEVELOP_128_6_827C35","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1016\/0092-8674(92)90611-F","article-title":"Targeted mutation of the DNA methyltransferase gene results in embryonic lethality","volume":"69","author":"Li","year":"1992","journal-title":"Cell"},{"key":"2023051200351529300_DEVELOP_128_6_827C36","doi-asserted-by":"publisher","first-page":"152","DOI":"10.1056\/NEJM198507183130304","article-title":"Antibody to cardiolipin as a predictor of fetal distress or death in pregnant patients with systemic lupus erythematosus","volume":"313","author":"Lockshin","year":"1985","journal-title":"N. Engl. J. Med"},{"key":"2023051200351529300_DEVELOP_128_6_827C37","doi-asserted-by":"publisher","first-page":"778","DOI":"10.1038\/42022","article-title":"Placental abnormalities in mouse embryos lacking the orphan nuclear receptor ERR-beta","volume":"388","author":"Luo","year":"1997","journal-title":"Nature"},{"key":"2023051200351529300_DEVELOP_128_6_827C38","doi-asserted-by":"publisher","first-page":"299","DOI":"10.1055\/s-2007-1002683","article-title":"Neonatal purpura fulminans due to homozygous protein C or protein S deficiencies","volume":"16","author":"Marlar","year":"1990","journal-title":"Semin. Thromb. Hemost"},{"key":"2023051200351529300_DEVELOP_128_6_827C39","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1083\/jcb.101.2.363","article-title":"Thrombomodulin is found on endothelium of arteries, veins, capillaries, and lymphatics, and on syncytiotrophoblast of human placenta","volume":"101","author":"Maruyama","year":"1985","journal-title":"J. Cell Biol"},{"key":"2023051200351529300_DEVELOP_128_6_827C40","doi-asserted-by":"publisher","first-page":"162","DOI":"10.1073\/pnas.96.1.162","article-title":"Placental failure in mice lacking the homeobox gene Dlx3","volume":"96","author":"Morasso","year":"1999","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023051200351529300_DEVELOP_128_6_827C41","first-page":"2391","article-title":"Production of homozygous mutant ES cells with a single targeting construct","volume":"12","author":"Mortensen","year":"1992","journal-title":"Mol. Cell Biol"},{"key":"2023051200351529300_DEVELOP_128_6_827C42","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1159\/000144801","article-title":"Development of trophoblast and placenta of the mouse. A reinvestigation with regard to the in vitro culture of mouse trophoblast and placenta","volume":"98","author":"Muntener","year":"1977","journal-title":"Acta Anat.(Basel)"},{"key":"2023051200351529300_DEVELOP_128_6_827C43","doi-asserted-by":"publisher","first-page":"8424","DOI":"10.1073\/pnas.90.18.8424","article-title":"Derivation of completely cell culture-derived mice from early-passage embryonic stem cells","volume":"90","author":"Nagy","year":"1993","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023051200351529300_DEVELOP_128_6_827C44","doi-asserted-by":"publisher","first-page":"179","DOI":"10.1016\/0028-2243(91)90021-C","article-title":"Histopathological findings in placentae from patients with intra-uterine fetal death and anti-phospholipid antibodies","volume":"41","author":"Out","year":"1991","journal-title":"Eur. J. Obstet. Gynecol. Reprod. Biol"},{"key":"2023051200351529300_DEVELOP_128_6_827C45","doi-asserted-by":"publisher","first-page":"71","DOI":"10.1016\/S0143-4004(81)80042-2","article-title":"Review article: trophoblast invasion and the establishment of haemochorial placentation in man and laboratory animals","volume":"2","author":"Pijnenborg","year":"1981","journal-title":"Placenta"},{"key":"2023051200351529300_DEVELOP_128_6_827C46","doi-asserted-by":"publisher","first-page":"154","DOI":"10.1056\/NEJM199707173370303","article-title":"Pregnancy loss in the antiphospholipid-antibody syndrome\u2013a possible thrombogenic mechanism","volume":"337","author":"Rand","year":"1997","journal-title":"New Engl. J. Med"},{"key":"2023051200351529300_DEVELOP_128_6_827C47","doi-asserted-by":"publisher","first-page":"4572","DOI":"10.1182\/blood.V91.12.4572","article-title":"Rapid activation of protein C by factor Xa and thrombin in the presence of polyanionic compounds","volume":"91","author":"Rezaie","year":"1998","journal-title":"Blood"},{"key":"2023051200351529300_DEVELOP_128_6_827C48","doi-asserted-by":"publisher","first-page":"912","DOI":"10.1056\/NEJM199504063321403","article-title":"Mutation in the gene coding for coagulation factor V and the risk of myocardial infarction, stroke, and venous thrombosis in apparently healthy men","volume":"332","author":"Ridker","year":"1995","journal-title":"New Engl. J. Med"},{"key":"2023051200351529300_DEVELOP_128_6_827C49","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1038\/ng0398-271","article-title":"The Hand1 bHLH transcription factor is essential for placentation and cardiac morphogenesis","volume":"18","author":"Riley","year":"1998","journal-title":"Nat. Genet"},{"key":"2023051200351529300_DEVELOP_128_6_827C50","doi-asserted-by":"publisher","first-page":"1555","DOI":"10.1056\/NEJM199905203402007","article-title":"Vascular-bed\u2013specific hemostasis and hypercoagulable states","volume":"340","author":"Rosenberg","year":"1999","journal-title":"New Engl. J. Med"},{"key":"2023051200351529300_DEVELOP_128_6_827C51","doi-asserted-by":"publisher","first-page":"1504","DOI":"10.1182\/blood.V85.6.1504.bloodjournal8561504","article-title":"High risk of thrombosis in patients homozygous for factor V Leiden (activated protein C resistance","volume":"85","author":"Rosendaal","year":"1995","journal-title":"Blood"},{"key":"2023051200351529300_DEVELOP_128_6_827C52","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1055\/s-2007-994176","article-title":"Placental pathology and antiphospholipid antibodies: a descriptive study","volume":"14","author":"Salafia","year":"1997","journal-title":"Am. J. Perinatol"},{"key":"2023051200351529300_DEVELOP_128_6_827C53","doi-asserted-by":"publisher","first-page":"1049","DOI":"10.1016\/0002-9378(95)91325-4","article-title":"Intrauterine growth restriction in infants of less than thirty-two weeks\u2019 gestation: associated placental pathologic features","volume":"173","author":"Salafia","year":"1995","journal-title":"Am. J. Obstet. Gynecol"},{"key":"2023051200351529300_DEVELOP_128_6_827C54","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1083\/jcb.144.1.151","article-title":"Absence of basement membranes after targeting the LAMC1 gene results in embryonic lethality due to failure of endoderm differentiation","volume":"144","author":"Smyth","year":"1999","journal-title":"J. Cell Biol"},{"key":"2023051200351529300_DEVELOP_128_6_827C55","doi-asserted-by":"publisher","first-page":"7597","DOI":"10.1073\/pnas.95.13.7597","article-title":"Prothrombin deficiency results in embryonic and neonatal lethality in mice","volume":"95","author":"Sun","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023051200351529300_DEVELOP_128_6_827C56","doi-asserted-by":"publisher","first-page":"1624","DOI":"10.1093\/humrep\/14.6.1624","article-title":"A possible role for activated protein C resistance in patients with first and second trimester pregnancy failure","volume":"14","author":"Tal","year":"1999","journal-title":"Hum. Reprod"},{"key":"2023051200351529300_DEVELOP_128_6_827C57","doi-asserted-by":"publisher","first-page":"2511","DOI":"10.1126\/science.286.5449.2511","article-title":"Leakage-resistant blood vessels in mice transgenically overexpressing angiopoietin-1","volume":"286","author":"Thurston","year":"1999","journal-title":"Science"},{"key":"2023051200351529300_DEVELOP_128_6_827C58","doi-asserted-by":"publisher","first-page":"6922","DOI":"10.1073\/pnas.94.13.6922","article-title":"Effect of tissue factor deficiency on mouse and tumor development","volume":"94","author":"Toomey","year":"1997","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023051200351529300_DEVELOP_128_6_827C59","doi-asserted-by":"publisher","first-page":"1583","DOI":"10.1182\/blood.V88.5.1583.1583","article-title":"Targeted disruption of the murine tissue factor gene results in embryonic lethality","volume":"88","author":"Toomey","year":"1996","journal-title":"Blood"},{"key":"2023051200351529300_DEVELOP_128_6_827C60","doi-asserted-by":"publisher","first-page":"702","DOI":"10.1038\/373702a0","article-title":"Placental defect and embryonic lethality in mice lacking hepatocyte growth factor\/scatter factor","volume":"373","author":"Uehara","year":"1995","journal-title":"Nature"},{"key":"2023051200351529300_DEVELOP_128_6_827C61","doi-asserted-by":"publisher","first-page":"11563","DOI":"10.1073\/pnas.94.21.11563","article-title":"A factor IX-deficient mouse model for hemophilia B gene therapy","volume":"94","author":"Wang","year":"1997","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023051200351529300_DEVELOP_128_6_827C62","doi-asserted-by":"publisher","first-page":"27176","DOI":"10.1074\/jbc.273.42.27176","article-title":"A study of the mechanism of inhibition of fibrinolysis by activated thrombin-activable fibrinolysis inhibitor","volume":"273","author":"Wang","year":"1998","journal-title":"J. Biol. Chem"},{"key":"2023051200351529300_DEVELOP_128_6_827C63","doi-asserted-by":"publisher","first-page":"55","DOI":"10.1016\/S0925-4773(99)00325-1","article-title":"The molecular basis of lung morphogenesis","volume":"92","author":"Warburton","year":"2000","journal-title":"Mech. Dev"},{"key":"2023051200351529300_DEVELOP_128_6_827C64","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1161\/01.RES.78.2.180","article-title":"Targeting of transgene expression to the vascular endothelium of mice by homologous recombination at the thrombomodulin locus","volume":"78","author":"Weiler-Guettler","year":"1996","journal-title":"Circ.Res"},{"key":"2023051200351529300_DEVELOP_128_6_827C65","doi-asserted-by":"publisher","first-page":"2271","DOI":"10.1242\/dev.122.7.2271","article-title":"Developmentally regulated gene expression of thrombomodulin in postimplantation mouse embryos","volume":"122","author":"Weiler-Guettler","year":"1996","journal-title":"Development"},{"key":"2023051200351529300_DEVELOP_128_6_827C66","doi-asserted-by":"publisher","first-page":"1983","DOI":"10.1172\/JCI2006","article-title":"A targeted point mutation in thrombomodulin generates viable mice with a prethrombotic state","volume":"101","author":"Weiler-Guettler","year":"1998","journal-title":"J. Clin. Invest"},{"key":"2023051200351529300_DEVELOP_128_6_827C67","doi-asserted-by":"publisher","first-page":"831","DOI":"10.1093\/hmg\/6.6.831","article-title":"Dystroglycan is essential for early embryonic development: disruption of Reichert\u2019s membrane in Dag1-null mice","volume":"6","author":"Williamson","year":"1997","journal-title":"Hum. Mol. Genet"},{"key":"2023051200351529300_DEVELOP_128_6_827C68","doi-asserted-by":"publisher","first-page":"7603","DOI":"10.1073\/pnas.95.13.7603","article-title":"Incomplete embryonic lethality and fatal neonatal hemorrhage caused by prothrombin deficiency in mice","volume":"95","author":"Xue","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2023051200351529300_DEVELOP_128_6_827C69","doi-asserted-by":"publisher","first-page":"6734","DOI":"10.1073\/pnas.120081897","article-title":"Prothrombotic phenotype of protein Z deficiency","volume":"97","author":"Yin","year":"2000","journal-title":"Proc. Natl. Acad. Sci. USA"}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/128\/6\/827\/2846198\/develop_128_6_827.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/128\/6\/827\/2846198\/develop_128_6_827.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,12]],"date-time":"2023-05-12T00:35:26Z","timestamp":1683851726000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/128\/6\/827\/41523\/Tissue-restricted-expression-of-thrombomodulin-in"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,3,15]]},"references-count":69,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2001,3,15]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.128.6.827","relation":{},"ISSN":["0950-1991","1477-9129"],"issn-type":[{"value":"0950-1991","type":"print"},{"value":"1477-9129","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,3,15]]}}}