{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T01:10:53Z","timestamp":1791335453566,"version":"4.3.1"},"reference-count":69,"publisher":"The Company of Biologists","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,5,15]]},"abstract":"<jats:p>We have used a morpholino-based knockdown approach to investigate the functions of a pair of zebrafish Hox gene duplicates, hoxb1a and hoxb1b, which are expressed during development of the hindbrain. We find that the zebrafish hoxb1 duplicates have equivalent functions to mouse Hoxb1 and its paralogue Hoxa1. Thus, we have revealed a \u2018function shuffling\u2019 among genes of paralogue group 1 during the evolution of vertebrates. Like mouse Hoxb1, zebrafish hoxb1a is required for migration of the VIIth cranial nerve branchiomotor neurons from their point of origin in hindbrain rhombomere 4 towards the posterior. By contrast, zebrafish hoxb1b, like mouse Hoxa1, is required for proper segmental organization of rhombomere 4 and the posterior hindbrain. Double knockdown experiments demonstrate that the zebrafish hoxb1 duplicates have partially redundant functions. However, using an RNA rescue approach, we reveal that these duplicated genes do not have interchangeable biochemical functions: only hoxb1a can properly pattern the VIIth cranial nerve. Despite this difference in protein function, we provide evidence that the hoxb1 duplicate genes were initially maintained in the genome because of complementary degenerative mutations in defined cis-regulatory elements.<\/jats:p>","DOI":"10.1242\/dev.129.10.2339","type":"journal-article","created":{"date-parts":[[2021,4,26]],"date-time":"2021-04-26T00:52:31Z","timestamp":1619398351000},"page":"2339-2354","source":"Crossref","is-referenced-by-count":153,"title":["Knockdown of duplicated zebrafish\n                    <i>hoxb1<\/i>\n                    genes reveals distinct roles in hindbrain patterning and a novel mechanism of duplicate gene retention"],"prefix":"10.1242","volume":"129","author":[{"given":"James M.","family":"McClintock","sequence":"first","affiliation":[{"name":"Committee on Developmental Biology, The University of Chicago, 1027 E 57th Street, Chicago, IL 60637, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mazen A.","family":"Kheirbek","sequence":"additional","affiliation":[{"name":"Department of Organismal Biology and Anatomy, The University of Chicago, 1027 E 57th Street, Chicago, IL 60637, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria E.","family":"Prince","sequence":"additional","affiliation":[{"name":"Committee on Developmental Biology, The University of Chicago, 1027 E 57th Street, Chicago, IL 60637, USA"},{"name":"Department of Organismal Biology and Anatomy, The University of Chicago, 1027 E 57th Street, Chicago, IL 60637, USA"},{"name":"Committees on Neurobiology and Evolutionary Biology, The University of Chicago, 1027 E 57th Street, Chicago, IL 60637, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042604180729300_ALEXANDRE-ETAL-1996","unstructured":"Alexandre, D., Clarke, J. D., Oxtoby, E., Yan, Y. L., Jowett, T. and Holder, N. (1996). Ectopic expression of Hoxa1 in the zebrafish alters the fate of the mandibular arch neural crest and phenocopies a retinoic acid-induced phenotype. Development122, 735-746."},{"key":"2021042604180729300_AMORES-ETAL-1998","doi-asserted-by":"crossref","unstructured":"Amores, A., Force, A., Yan, Y.-L., Amemiya, C., Fritz, A., Ho, R. K., Joly, L., Langeland, J., Prince, V., Wang, Y-L. et al. (1998). Genome duplications in vertebrate evolution: evidence from zebrafish Hox clusters. Science282, 1711-1714.","DOI":"10.1126\/science.282.5394.1711"},{"key":"2021042604180729300_APARICIO-ETAL-1997","doi-asserted-by":"crossref","unstructured":"Aparicio, S., Hawker, K., Cottage, A., Mikawa, Y., Zuo, L., Venkatesh, B., Chen, E., Krumlauf, R. and Brenner, S. (1997). Organization of the Fugu rubripes Hox clusters: evidence for continuing evolution of vertebrate Hox complexes. Nat. 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Development118, 1063-1075."},{"key":"2021042604180729300_CARROLL-1988","unstructured":"Carroll, R. L. (1988). Vertebrate Paleontology and Evolution. New York: W.H. Freeman."},{"key":"2021042604180729300_CHANDRASEKHAR-ETAL-1997","unstructured":"Chandrasekhar, A., Moens, C., Warren, J. T., Jr, Kimmel, C. B. and Kuwada, J. Y. (1997). Development of branchiomotor neurons in zebra fish. Development124, 2633-2644."},{"key":"2021042604180729300_CHISAKA-ETAL-1992","doi-asserted-by":"crossref","unstructured":"Chisaka, O., Musci, T. E. and Capecchi, M. R. (1992). Developmental defects of the ear, cranial nerves and hindbrain resulting from targeted disruption of the mouse homeobox gene Hox 1.6. Nature355, 516-520.","DOI":"10.1038\/355516a0"},{"key":"2021042604180729300_CONDIE-AND-CAPECCHI-1993","unstructured":"Condie, B. G. and Capecchi, M. R. 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