{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T08:10:26Z","timestamp":1779178226014,"version":"3.51.4"},"reference-count":56,"publisher":"The Company of Biologists","issue":"17","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2002,9,1]]},"abstract":"<jats:p>The fused gene encodes a serine\/threonine kinase involved in Hedgehog signal transduction during Drosophila embryo and larval imaginal disc development. Additionally, fused mutant females exhibit reduced fecundity that we report here to be associated with defects in three aspects of egg chamber formation: encapsulation of germline cysts by prefollicular cells in the germarium, interfollicular stalk morphogenesis and oocyte posterior positioning. Using clonal analysis we show that fused is required cell autonomously in prefollicular and pre-stalk cells to control their participation in these aspects of egg chamber formation. In contrast to what has been found for Hedgehog and other known components of Hedgehog signal transduction, we show that fused does not play a role in the regulation of somatic stem cell proliferation. However, genetic interaction studies, as well as the analysis of the effects of a partial reduction in Hedgehog signaling in the ovary, indicate that fused acts in the classical genetic pathway for Hedgehog signal transduction which is necessary for somatic cell differentiation during egg chamber formation. Therefore, we propose a model in which Hedgehog signals at least twice in germarial somatic cells: first, through a fused-independent pathway to control somatic stem cell proliferation; and second, through a classical fused-dependent pathway to regulate prefollicular cell differentiation.<\/jats:p>","DOI":"10.1242\/dev.129.17.4111","type":"journal-article","created":{"date-parts":[[2021,4,25]],"date-time":"2021-04-25T13:16:10Z","timestamp":1619356570000},"page":"4111-4124","source":"Crossref","is-referenced-by-count":14,"title":["Fused-dependent Hedgehog signal transduction is required for somatic cell differentiation during<i>Drosophila<\/i>egg chamber formation"],"prefix":"10.1242","volume":"129","author":[{"given":"Florence","family":"Besse","sequence":"first","affiliation":[{"name":"Institut Jacques Monod, UMR 7592 \u2013 CNRS\/Universite\u0301 Pierre et Marie Curie\/Universite\u0301 Denis Diderot, Laboratoire de Ge\u0301ne\u0301tique du De\u0301veloppement et Evolution, 2-4 place Jussieu, 75251 Paris Cedex 05, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Denise","family":"Busson","sequence":"additional","affiliation":[{"name":"Institut Jacques Monod, UMR 7592 \u2013 CNRS\/Universite\u0301 Pierre et Marie Curie\/Universite\u0301 Denis Diderot, Laboratoire de Ge\u0301ne\u0301tique du De\u0301veloppement et Evolution, 2-4 place Jussieu, 75251 Paris Cedex 05, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne-Marie","family":"Pret","sequence":"additional","affiliation":[{"name":"Institut Jacques Monod, UMR 7592 \u2013 CNRS\/Universite\u0301 Pierre et Marie Curie\/Universite\u0301 Denis Diderot, Laboratoire de Ge\u0301ne\u0301tique du De\u0301veloppement et Evolution, 2-4 place Jussieu, 75251 Paris Cedex 05, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","reference":[{"key":"2021042511353990000_ALVES-ETAL-1998","doi-asserted-by":"crossref","unstructured":"Alves, G., Limbourg, B. B., Tricoire, H., Brissard, Z. J., Lamour, I. C. and Busson, D. (1998). Modulation of Hedgehog target gene expression by the Fused serine-threonine kinase in wing imaginal discs. Mech. Dev.78, 17-31.","DOI":"10.1016\/S0925-4773(98)00130-0"},{"key":"2021042511353990000_BIER-ETAL-1989","doi-asserted-by":"crossref","unstructured":"Bier, E., Vaessin, H., Shepherd, S., Lee, K., McCall, K., Barbel, S., Ackerman, L., Carretto, R., Uemura, T., Grell, E. et al. ( 1989). Searching for pattern and mutation in the Drosophila genome with a P-lacZ vector. Genes Dev.3, 1273-1287.","DOI":"10.1101\/gad.3.9.1273"},{"key":"2021042511353990000_BUSSON-ETAL-1988","doi-asserted-by":"crossref","unstructured":"Busson, D., Limbourg-Bouchon, B., Mariol, M. C., Pre\u0301at, T. and Lamour-Isnard, C. (1988). Genetic analysis of viable and lethal fused mutants of Drosophila melanogaster. Roux\u2019s Arch. Dev. Biol.197, 221-230.","DOI":"10.1007\/BF02439429"},{"key":"2021042511353990000_BYERS-ETAL-1987","unstructured":"Byers, T. J., Dubreuil, R., Branton, D., Kiehart, D. P. and Goldstein, L. S. (1987). Drosophila spectrin. II. Conserved features of the alpha-subunit are revealed by analysis of cDNA clones and fusion proteins. J. Cell Biol.105, 2103-2110."},{"key":"2021042511353990000_DAHMANN-AND-BASLER-2000","doi-asserted-by":"crossref","unstructured":"Dahmann, C. and Basler, K. (2000). Opposing transcriptional outputs of Hedgehog signaling and engrailed control compartmental cell sorting at the Drosophila A\/P boundary. Cell100, 411-422.","DOI":"10.1016\/S0092-8674(00)80677-7"},{"key":"2021042511353990000_DESHPANDE-ETAL-2001","doi-asserted-by":"crossref","unstructured":"Deshpande, G., Swanhart, L., Chiang, P. and Schedl, P. (2001). Hedgehog signaling in germ cell migration. Cell106, 759-769.","DOI":"10.1016\/S0092-8674(01)00488-3"},{"key":"2021042511353990000_DOERFLINGER-ETAL-1999","doi-asserted-by":"crossref","unstructured":"Doerflinger, H., Lepesant, J. A. and Yanicostas, C. (1999). Differential expression of the Drosophila zinc finger gene jim in the follicular epithelium. Mech. Dev.86, 177-182.","DOI":"10.1016\/S0925-4773(99)00109-4"},{"key":"2021042511353990000_DUFFY-ETAL-1998","doi-asserted-by":"crossref","unstructured":"Duffy, J. B., Harrison, D. A. and Perrimon, N. (1998). Identifying loci required for follicular patterning using directed mosaics. Development125, 2263-2271.","DOI":"10.1242\/dev.125.12.2263"},{"key":"2021042511353990000_FORBES-ETAL-1996A","doi-asserted-by":"crossref","unstructured":"Forbes, A. J., Lin, H., Ingham, P. W. and Spradling, A. C. (1996a). hedgehog is required for the proliferation and specification of ovarian somatic cells prior to egg chamber formation in Drosophila. Development122, 1125-1135.","DOI":"10.1242\/dev.122.4.1125"},{"key":"2021042511353990000_FORBES-ETAL-1996B","doi-asserted-by":"crossref","unstructured":"Forbes, A. J., Spradling, A. C., Ingham, P. W. and Lin, H. (1996b). The role of segment polarity genes during early oogenesis in Drosophila. Development122, 3283-3294.","DOI":"10.1242\/dev.122.10.3283"},{"key":"2021042511353990000_GODT-AND-TEPASS-1998","doi-asserted-by":"crossref","unstructured":"Godt, D. and Tepass, U. (1998). Drosophila oocyte localization is mediated by differential cadherin-based adhesion. Nature395, 387-391.","DOI":"10.1038\/26493"},{"key":"2021042511353990000_GONZALEZ-AND-ST-JOHNSTON-1998","doi-asserted-by":"crossref","unstructured":"Gonzalez, R. A. and St Johnston, D. (1998). The Drosophila AP axis is polarised by the cadherin-mediated positioning of the oocyte. Development125, 3635-3644.","DOI":"10.1242\/dev.125.18.3635"},{"key":"2021042511353990000_GOODE-ETAL-1996A","doi-asserted-by":"crossref","unstructured":"Goode, S., Melnick, M., Chou, T. B. and Perrimon, N. (1996a). The neurogenic genes egghead and brainiac define a novel signaling pathway essential for epithelial morphogenesis during Drosophila oogenesis. Development122, 3863-3879.","DOI":"10.1242\/dev.122.12.3863"},{"key":"2021042511353990000_GOODE-ETAL-1996B","doi-asserted-by":"crossref","unstructured":"Goode, S., Morgan, M., Liang, Y. P. and Mahowald, A. P. (1996b). brainiac encodes a novel, putative secreted protein that cooperates with Grk TGF alpha in the genesis of the follicular epithelium. Dev. Biol.178, 35-50.","DOI":"10.1006\/dbio.1996.0196"},{"key":"2021042511353990000_GOODE-AND-PERRIMON-1997","doi-asserted-by":"crossref","unstructured":"Goode, S. and Perrimon, N. (1997). Inhibition of patterned cell shape change and cell invasion by Discs large during Drosophila oogenesis. Genes Dev.11, 2532-2544.","DOI":"10.1101\/gad.11.19.2532"},{"key":"2021042511353990000_GOODE-ETAL-1992","doi-asserted-by":"crossref","unstructured":"Goode, S., Wright, D. and Mahowald, A. P. (1992). The neurogenic locus brainiac cooperates with the Drosophila EGF receptor to establish the ovarian follicle and to determine its dorsal-ventral polarity. Development116, 177-192.","DOI":"10.1242\/dev.116.1.177"},{"key":"2021042511353990000_GRAMMONT-ETAL-1997","doi-asserted-by":"crossref","unstructured":"Grammont, M., Dastugue, B. and Couderc, J. L. (1997). The Drosophila toucan (toc) gene is required in germline cells for the somatic cell patterning during oogenesis. Development124, 4917-4926.","DOI":"10.1242\/dev.124.24.4917"},{"key":"2021042511353990000_GRAMMONT-AND-IRVINE-2001","unstructured":"Grammont, M. and Irvine, K. D. (2001). fringe and Notch specify polar cell fate during Drosophila oogenesis. Development128, 2243-2253."},{"key":"2021042511353990000_INGHAM-1993","doi-asserted-by":"crossref","unstructured":"Ingham, P. W. (1993). Localized hedgehog activity controls spatial limits of wingless transcription in the Drosophila embryo. Nature366, 560-562.","DOI":"10.1038\/366560a0"},{"key":"2021042511353990000_KING-1970","unstructured":"King, R. C. (1970). Ovarian Development in Drosophila melanogaster. New York: Academic Press."},{"key":"2021042511353990000_LIMBOURG-BOUCHON-ETAL-1991","doi-asserted-by":"crossref","unstructured":"Limbourg-Bouchon, B., Busson, D. and Lamour-Isnard, C. (1991). Interactions between fused, a segment-polarity gene in Drosophila, and other segmentation genes. Development112, 417-429.","DOI":"10.1242\/dev.112.2.417"},{"key":"2021042511353990000_LIN-AND-SPRADLING-1993","doi-asserted-by":"crossref","unstructured":"Lin, H. and Spradling, A. C. (1993). Germline stem cell division and egg chamber development in transplanted Drosophila germaria. Dev. Biol.159, 140-152.","DOI":"10.1006\/dbio.1993.1228"},{"key":"2021042511353990000_LIN-ETAL-1994","doi-asserted-by":"crossref","unstructured":"Lin, H., Yue, L. and Spradling, A. C. (1994). The Drosophila fusome, a germline-specific organelle, contains membrane skeletal proteins and functions in cyst formation. Development120, 947-956.","DOI":"10.1242\/dev.120.4.947"},{"key":"2021042511353990000_LOPEZ-SCHIER-AND-ST-JOHNSTON-2001","doi-asserted-by":"crossref","unstructured":"Lopez-Schier, H. and St Johnston, D. (2001). Delta signaling from the germ line controls the proliferation and differentiation of the somatic follicle cells during Drosophila oogenesis. Genes Dev.15, 1393-1405.","DOI":"10.1101\/gad.200901"},{"key":"2021042511353990000_MA-ETAL-1993","doi-asserted-by":"crossref","unstructured":"Ma, C., Zhou, Y., Beachy, P. A. and Moses, K. (1993). The segment polarity gene hedgehog is required for progression of the morphogenetic furrow in the developing Drosophila eye. Cell75, 927-938.","DOI":"10.1016\/0092-8674(93)90536-Y"},{"key":"2021042511353990000_MARGOLIS-AND-SPRADLING-1995","doi-asserted-by":"crossref","unstructured":"Margolis, J. and Spradling, A. (1995). Identification and behavior of epithelial stem cells in the Drosophila ovary. Development121, 3797-3807.","DOI":"10.1242\/dev.121.11.3797"},{"key":"2021042511353990000_MCKEARIN-AND-OHLSTEIN-1995","doi-asserted-by":"crossref","unstructured":"McKearin, D. and Ohlstein, B. (1995). A role for the Drosophila Bag-of-marbles protein in the differentiation of cystoblasts from germline stem cells. Development121, 2937-2947.","DOI":"10.1242\/dev.121.9.2937"},{"key":"2021042511353990000_METHOT-AND-BASLER-1999","doi-asserted-by":"crossref","unstructured":"Methot, N. and Basler, K. (1999). Hedgehog controls limb development by regulating the activities of distinct transcriptional activator and repressor forms of Cubitus interruptus. Cell96, 819-831.","DOI":"10.1016\/S0092-8674(00)80592-9"},{"key":"2021042511353990000_MURONE-ETAL-1999","doi-asserted-by":"crossref","unstructured":"Murone, M., Rosenthal, A. and de Sauvage, F. (1999). Hedgehog signal transduction: from flies to vertebrates. Exp. Cell Res.253, 25-33.","DOI":"10.1006\/excr.1999.4676"},{"key":"2021042511353990000_NEUFELD-ETAL-1998","doi-asserted-by":"crossref","unstructured":"Neufeld, T. P., de la Cruz, A. F., Johnston, L. A. and Edgar, B. A. (1998). Coordination of growth and cell division in the Drosophila wing. Cell93, 1183-1193.","DOI":"10.1016\/S0092-8674(00)81462-2"},{"key":"2021042511353990000_NIEWIADOMSKA-ETAL-1999","doi-asserted-by":"crossref","unstructured":"Niewiadomska, P., Godt, D. and Tepass, U. (1999). DE-Cadherin is required for intercellular motility during Drosophila oogenesis. J Cell Biol144, 533-547.","DOI":"10.1083\/jcb.144.3.533"},{"key":"2021042511353990000_ODA-ETAL-1994","doi-asserted-by":"crossref","unstructured":"Oda, H., Uemura, T., Harada, Y., Iwai, Y. and Takeichi, M. (1994). A Drosophila homolog of cadherin associated with armadillo and essential for embryonic cell-cell adhesion. Dev. Biol.165, 716-726.","DOI":"10.1006\/dbio.1994.1287"},{"key":"2021042511353990000_PIGNONI-AND-ZIPURSKI-1997","doi-asserted-by":"crossref","unstructured":"Pignoni, F. and Zipurski, S. L. (1997). Induction of Drosophila eye development by Decapentaplegic. Development124, 271-278.","DOI":"10.1242\/dev.124.2.271"},{"key":"2021042511353990000_PREACUTEAT-1992","doi-asserted-by":"crossref","unstructured":"Pre\u0301at, T. (1992). Characterization of Suppressor of fused, a complete suppressor of the fused segment polarity gene of Drosophila melanogaster. Genetics132, 725-736.","DOI":"10.1093\/genetics\/132.3.725"},{"key":"2021042511353990000_PREACUTEAT-ETAL-1993","doi-asserted-by":"crossref","unstructured":"Pre\u0301at, T., Therond, P., Limbourg-Bouchon, B., Pham, A., Tricoire, H., Busson, D. and Lamour-Isnard, C. (1993). Segmental polarity in Drosophila melanogaster: genetic dissection of fused in a Suppressor of fused background reveals interaction with costal-2. Genetics135, 1047-1062.","DOI":"10.1093\/genetics\/135.4.1047"},{"key":"2021042511353990000_RAMIREZ-WEBER-ETAL-2000","doi-asserted-by":"crossref","unstructured":"Ramirez-Weber, F. A., Casso, D. J., Aza-Blanc, P., Tabata, T. and Kornberg, T. B. (2000). Hedgehog signal transduction in the posterior compartment of the Drosophila wing imaginal disc. Mol. Cell6, 479-485.","DOI":"10.1016\/S1097-2765(00)00046-0"},{"key":"2021042511353990000_ROBBINS-ETAL-1997","doi-asserted-by":"crossref","unstructured":"Robbins, D. J., Nybakken, K. E., Kobayashi, R., Sisson, J. C., Bishop, J. M. and The\u0301rond, P. P. (1997). Hedhehog elicits signal transduction by means of a large complex containing the kinesin-related protein Costal2. Cell90, 225-234.","DOI":"10.1016\/S0092-8674(00)80331-1"},{"key":"2021042511353990000_RUBSAM-ETAL-1998","doi-asserted-by":"crossref","unstructured":"Rubsam, R., Hollmann, M., Simmerl, E., Lammermann, U., Schafer, M. A., Buning, J. and Schafer, U. (1998). The egghead gene product influences oocyte differentiation by follicle cell-germ cell interactions in Drosophila melanogaster. Mech. Dev.72, 131-140.","DOI":"10.1016\/S0925-4773(98)00023-9"},{"key":"2021042511353990000_RUOHOLA-ETAL-1991","doi-asserted-by":"crossref","unstructured":"Ruohola, H., Bremer, K. A., Baker, D., Swedlow, J. R., Jan, L. Y. and Jan, Y. N. (1991). Role of neurogenic genes in establishment of follicle cell fate and oocyte polarity during oogenesis in Drosophila. Cell66, 433-449.","DOI":"10.1016\/0092-8674(81)90008-8"},{"key":"2021042511353990000_SANCHEZ-HERRERO-ETAL-1996","doi-asserted-by":"crossref","unstructured":"Sanchez-Herrero, E., Couso, J. P., Capdevila, J. and Guerrero, I. (1996). The fu gene discriminates between pathways to control dpp expression in Drosophila imaginal discs. Mech. Dev.55, 159-170.","DOI":"10.1016\/0925-4773(96)00498-4"},{"key":"2021042511353990000_SISSON-ETAL-1997","doi-asserted-by":"crossref","unstructured":"Sisson, J. C., Ho, K. S., Suyama, K. and Scott, M. P. (1997). Costal2, a novel kinesin-related protein in the Hedgehog signaling pathway. Cell90, 235-245.","DOI":"10.1016\/S0092-8674(00)80332-3"},{"key":"2021042511353990000_SPRADLING-1993","unstructured":"Spradling, A. (1993). Developmental genetics of oogenesis. In Development of Drosophila melanogaster (ed. M. Bate and A. Martinez-Arias), pp. 1-70. Cold Spring Harbor, New York: Cold Spring Harbor Laboratory Press."},{"key":"2021042511353990000_SPRADLING-ETAL-1997","unstructured":"Spradling, A. C., de Cuevas, M., Drummond, B. D., Keyes, L., Lilly, M., Pepling, M. and Xie, T. (1997). The Drosophila germarium: stem cells, germ line cysts, and oocytes. Cold Spring Harb. Symp. Quant. Biol.62, 25-34."},{"key":"2021042511353990000_SUZUKI-AND-SAIGO-2000","doi-asserted-by":"crossref","unstructured":"Suzuki, T. and Saigo, K. (2000). Transcriptional regulation of atonal required for Drosophila larval eye development by concerted action of Eyes absent, Sine oculis and Hedgehog signaling independent of Fused kinase and Cubitus interruptus. Development127, 1531-1540.","DOI":"10.1242\/dev.127.7.1531"},{"key":"2021042511353990000_TABATA-AND-KORNBERG-1994","doi-asserted-by":"crossref","unstructured":"Tabata, T. and Kornberg, T. B. (1994). Hedgehog is a signaling protein with a key role in patterning Drosophila imaginal discs. Cell76, 89-102.","DOI":"10.1016\/0092-8674(94)90175-9"},{"key":"2021042511353990000_TANENTZAPF-ETAL-2000","doi-asserted-by":"crossref","unstructured":"Tanentzapf, G., Smith, C., McGlade, J. and Tepass, U. (2000). Apical, lateral, and basal polarization cues contribute to the development of the follicular epithelium during Drosophila oogenesis. J. Cell Biol.151, 891-904.","DOI":"10.1083\/jcb.151.4.891"},{"key":"2021042511353990000_THEROND-ETAL-1993","doi-asserted-by":"crossref","unstructured":"Therond, P., Busson, D., Guillemet, E., Limbourg-Bouchon, B., Preat, T., Terracol, R., Tricoire, H. and Lamour-Isnard, C. (1993). Molecular organisation and expression pattern of the segment polarity gene fused of Drosophila melanogaster. Mech. Dev. 44, 65-80.","DOI":"10.1016\/0925-4773(93)90017-R"},{"key":"2021042511353990000_THEROND-ETAL-1996","doi-asserted-by":"crossref","unstructured":"Therond, P., Alves, G., Limbourg, B. B., Tricoire, H., Guillemet, E., Brissard-Zahraoui, J., Lamour, I. C. and Busson, D. (1996). Functional domains of fused, a serine-threonine kinase required for signaling in Drosophila. Genetics142, 1181-1198.","DOI":"10.1093\/genetics\/142.4.1181"},{"key":"2021042511353990000_THEROND-ETAL-1999","doi-asserted-by":"crossref","unstructured":"Therond, P. P., Limbourg, B. B., Gallet, A., Dussilol, F., Pietri, T., van den Heuvel, M. and Tricoire, H. (1999). Differential requirements of the Fused kinase for Hedgehog signalling in the Drosophila embryo. Development126, 4039-4051.","DOI":"10.1242\/dev.126.18.4039"},{"key":"2021042511353990000_TWOROGER-ETAL-1999","doi-asserted-by":"crossref","unstructured":"Tworoger, M., Larkin, M. K., Bryant, Z. and Ruohola, B. H. (1999). Mosaic analysis in the drosophila ovary reveals a common hedgehog-inducible precursor stage for stalk and polar cells. Genetics151, 739-748.","DOI":"10.1093\/genetics\/151.2.739"},{"key":"2021042511353990000_VERVOORT-ETAL-1999","doi-asserted-by":"crossref","unstructured":"Vervoort, M., Crozatier, M., Valle, D. and Vincent, A. (1999). The COE transcription factor Collier is a mediator of short-range Hedgehog-induced patterning of the Drosophila wing. Curr. Biol.9, 632-639.","DOI":"10.1016\/S0960-9822(99)80285-1"},{"key":"2021042511353990000_VIED-AND-HORABIN-2001","unstructured":"Vied, C. and Horabin, J. I. (2001). The sex determination master switch, Sex-lethal, responds to Hedgehog signaling in the Drosophila germline. Development128, 2649-2660."},{"key":"2021042511353990000_WIESCHAUS-AND-SZABAD-1979","doi-asserted-by":"crossref","unstructured":"Wieschaus, E. and Szabad, J. (1979). The development and function of the female germ line in Drosophila melanogaster: a cell lineage study. Dev. Biol.68, 29-46.","DOI":"10.1016\/0012-1606(79)90241-0"},{"key":"2021042511353990000_ZARNESCU-AND-THOMAS-1999","doi-asserted-by":"crossref","unstructured":"Zarnescu, D. C. and Thomas, G. H. (1999). Apical spectrin is essential for epithelial morphogenesis but not apicobasal polarity in Drosophila. J. Cell Biol.146, 1075-1086.","DOI":"10.1083\/jcb.146.5.1075"},{"key":"2021042511353990000_ZHANG-AND-KALDERON-2000","doi-asserted-by":"crossref","unstructured":"Zhang, Y. and Kalderon, D. (2000). Regulation of cell proliferation and patterning in Drosophila oogenesis by Hedgehog signaling. Development127, 2165-2176.","DOI":"10.1242\/dev.127.10.2165"},{"key":"2021042511353990000_ZHANG-AND-KALDERON-2001","doi-asserted-by":"crossref","unstructured":"Zhang, Y. and Kalderon, D. (2001). Hedgehog acts as a somatic stem cell factor in the Drosophila ovary. Nature410, 599-604.","DOI":"10.1038\/35069099"}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/129\/17\/4111\/1139700\/4111.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/129\/17\/4111\/1139700\/4111.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,25]],"date-time":"2022-12-25T11:27:29Z","timestamp":1671967649000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/129\/17\/4111\/17828\/Fused-dependent-Hedgehog-signal-transduction-is"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,9,1]]},"references-count":56,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2002,9,1]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.129.17.4111","relation":{},"ISSN":["1477-9129","0950-1991"],"issn-type":[{"value":"1477-9129","type":"electronic"},{"value":"0950-1991","type":"print"}],"subject":[],"published":{"date-parts":[[2002,9,1]]}}}