{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T22:24:51Z","timestamp":1790375091012,"version":"4.1.0"},"reference-count":48,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2016,11,15]],"date-time":"2016-11-15T00:00:00Z","timestamp":1479168000000},"content-version":"vor","delay-in-days":319,"URL":"http:\/\/www.biologists.com\/user-licence-1-1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2016,1,1]]},"abstract":"<jats:p>Sebocytes, cells characterized by lipid accumulation leading to cell disruption, can be found in hair follicle-associated sebaceous glands (SGs) or in free SGs such as the Meibomian glands in the eyelids. Because genetic tools allowing sebocyte targeting while maintaining intact epidermal lipids are lacking, the relevance of sebaceous lipids in health and disease remains poorly understood. Using Scd3, a gene expressed exclusively in mature sebocytes, we established a mouse line with sebocyte-specific expression of cre recombinase. Both RT-PCR analysis and crossing into Rosa26-LacZ reporter mice and KrasG12D mice confirmed cre activity specifically in SGs, with no activity in other skin compartments. Importantly, loss of SCD3 function did not cause detectable phenotypical alterations, endorsing the usefulness of Scd3cre mice for further functional studies. Scd3cre-induced, diphtheria chain A toxin-mediated depletion of sebaceous lipids resulted in impaired water repulsion and thermoregulation, increased rates of UVB-induced epidermal apoptosis, and caused a severe pathology of the ocular surface resembling Meibomian gland dysfunction. This novel mouse line will be useful for further investigating the roles of sebaceous lipids in skin and eye integrity.<\/jats:p>","DOI":"10.1242\/dev.132753","type":"journal-article","created":{"date-parts":[[2016,3,18]],"date-time":"2016-03-18T02:40:25Z","timestamp":1458268825000},"source":"Crossref","is-referenced-by-count":30,"title":["Sebaceous lipids are essential for water repulsion, protection against UVB-induced apoptosis, and ocular integrity in mice"],"prefix":"10.1242","author":[{"given":"Maik","family":"Dahlhoff","sequence":"first","affiliation":[{"name":"Institute of Molecular Animal Breeding and Biotechnology, Gene Center, LMU Munich, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuela","family":"Camera","sequence":"additional","affiliation":[{"name":"Laboratory of Cutaneous Physiopathology and Integrated Center of Metabolomics Research, San Gallicano Dermatologic Institute, IRCCS, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Sch\u00e4fer","sequence":"additional","affiliation":[{"name":"Institute of Molecular Health Sciences, ETH Zurich, Zurich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela","family":"Emrich","sequence":"additional","affiliation":[{"name":"Institute of Veterinary Pathology, Center for Clinical Veterinary Medicine, LMU Munich, 80539 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dieter","family":"Riethmacher","sequence":"additional","affiliation":[{"name":"School of Medicine, Nazarbayev University, Astana, Kazachstan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"April","family":"Foster","sequence":"additional","affiliation":[{"name":"Centre for Dermatology Research, Institute of Inflammation and Repair, University of Manchester, Manchester, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf","family":"Paus","sequence":"additional","affiliation":[{"name":"Centre for Dermatology Research, Institute of Inflammation and Repair, University of Manchester, Manchester, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlon R.","family":"Schneider","sequence":"additional","affiliation":[{"name":"Institute of Molecular Animal Breeding and Biotechnology, Gene Center, LMU Munich, Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042608343698500_DEV132753C1","doi-asserted-by":"publisher","first-page":"933","DOI":"10.1111\/j.1751-1097.2010.00744.x","article-title":"GammaH2AX, an accurate marker that analyzes UV genotoxic effects on human keratinocytes and on human skin","volume":"86","author":"Barnes","year":"2010","journal-title":"Photochem. 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