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Here, we show how Wnt signaling controls epithelial morphogenetic changes and differentiation using the salivary gland as a model. Experiments using genetically manipulated mice and organ cultures revealed that Wnt signaling at an early stage (E12-E15) of submandibular salivary gland (SMG) development inhibits end bud morphogenesis and differentiation into proacini by suppressing KIT expression through the up-regulation of Myb transcription factor, and concomitantly increased the expression of distal progenitor markers. In addition, Wnt signaling at the SMG development early stage promoted end bud cell proliferation, leading to duct formation. In contrast, Wnt signaling reduction at a late stage (E16-E18) of SMG development promoted end bud maturation and suppressed duct formation. Thus, Wnt signaling controls the timing of SMG organogenesis by keeping end bud cells in an undifferentiated bipotent state.<\/jats:p>","DOI":"10.1242\/dev.134486","type":"journal-article","created":{"date-parts":[[2016,5,10]],"date-time":"2016-05-10T00:54:01Z","timestamp":1462841641000},"source":"Crossref","is-referenced-by-count":31,"title":["The Wnt-Myb pathway suppresses KIT expression to control the timing of salivary proacinar differentiation and duct formation"],"prefix":"10.1242","author":[{"given":"Shinji","family":"Matsumoto","sequence":"first","affiliation":[{"name":"Departments of Molecular Biology and Biochemistry, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takayuki","family":"Kurimoto","sequence":"additional","affiliation":[{"name":"Departments of Molecular Biology and Biochemistry, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan"},{"name":"The First Department of Oral and Maxillofacial Surgery, Graduate School of Dentistry, Osaka University, 1-8 Yamadaoka, Suita, Osaka 565-0871, Japan"},{"name":"Co-first author."}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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