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PDGFR\u03b1 is a marker of adipocyte precursors, and increased PDGFR\u03b1 activity causes adipose tissue fibrosis in adult mice. However, the function of PDGFR\u03b1 during adipose tissue organogenesis is unknown. Here, by analyzing mice with juxtamembrane or kinase domain point mutations that increase PDGFR\u03b1 activity (V561D or D842V), we found that PDGFR\u03b1 activation inhibits embryonic white adipose tissue organogenesis in a tissue-autonomous manner. By lineage tracing analysis, we also found that collagen-expressing precursor fibroblasts differentiate into white adipocytes in the embryo. PDGFR\u03b1 inhibited the formation of adipocytes from these precursors while favoring the formation of stromal fibroblasts. This imbalance between adipocytes and stromal cells was accompanied by overexpression of the cell fate regulator Zfp521. PDGFR\u03b1 activation also inhibited the formation of juvenile beige adipocytes in the inguinal fat pad. Our data highlight the importance of balancing stromal versus adipogenic cell expansion during white adipose tissue development, with PDGFR\u03b1 activity coordinating this crucial process in the embryo.<\/jats:p>","DOI":"10.1242\/dev.135962","type":"journal-article","created":{"date-parts":[[2017,1,3]],"date-time":"2017-01-03T12:58:27Z","timestamp":1483448307000},"page":"83-94","source":"Crossref","is-referenced-by-count":84,"title":["PDGFR\u03b1 controls the balance of stromal and adipogenic cells during adipose tissue organogenesis"],"prefix":"10.1242","volume":"144","author":[{"given":"Chengyi","family":"Sun","sequence":"first","affiliation":[{"name":"Cardiovascular Biology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA"},{"name":"Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William L.","family":"Berry","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2168-7836","authenticated-orcid":false,"given":"Lorin E.","family":"Olson","sequence":"additional","affiliation":[{"name":"Cardiovascular Biology Program, Oklahoma Medical Research Foundation, Oklahoma City, OK 73104, USA"},{"name":"Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2021042608511180000_DEV135962C1","doi-asserted-by":"publisher","first-page":"8015","DOI":"10.1128\/MCB.22.22.8015-8025.2002","article-title":"Early B-cell factor (O\/E-1) is a promoter of adipogenesis and involved in control of genes important for terminal adipocyte differentiation","volume":"22","author":"\u00c5kerblad","year":"2002","journal-title":"Mol. 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