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Self-organisation can also reform embryonic organ structure after tissue disruption. It has previously been demonstrated that dissociated embryonic kidneys can recreate component epithelial and mesenchymal relationships sufficient to allow continued kidney morphogenesis. Here we investigate the timing and underlying mechanisms driving self-organisation after dissociation of the embryonic kidney using time-lapse imaging, high-resolution confocal analyses and mathematical modelling. Organotypic self-organisation sufficient for nephron initiation was observed within a 24 hour period. This involved cell movement with structure emerging after the clustering of ureteric epithelial cells, a process consistent with models of random cell movement with preferential cell adhesion. Ureteric epithelialisation rapidly followed the formation of ureteric cell clusters with the reformation of nephron forming niches representing a later event. Disruption of P-cadherin interactions was seen to impair this ureteric epithelial cell clustering without affecting epithelial maturation. This understanding may facilitate improved regulation of patterning within organoids and facilitate kidney engineering approaches guided by cell-cell self-organisation.<\/jats:p>","DOI":"10.1242\/dev.140228","type":"journal-article","created":{"date-parts":[[2017,2,8]],"date-time":"2017-02-08T01:25:39Z","timestamp":1486517139000},"source":"Crossref","is-referenced-by-count":20,"title":["Self-organisation after embryonic kidney dissociation is driven via selective adhesion of ureteric epithelial cells."],"prefix":"10.1242","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5945-9575","authenticated-orcid":false,"given":"J. 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