{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T11:14:58Z","timestamp":1740136498612,"version":"3.37.3"},"reference-count":29,"publisher":"The Company of Biologists","issue":"5","license":[{"start":{"date-parts":[[2017,9,1]],"date-time":"2017-09-01T00:00:00Z","timestamp":1504224000000},"content-version":"vor","delay-in-days":184,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/100004440","name":"Wellcome Trust","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,3,1]]},"abstract":"<jats:p>A core structural and functional motif of the vertebrate central nervous system is discrete clusters of neurons or \u2018nuclei\u2019. Yet the developmental mechanisms underlying this fundamental mode of organisation are largely unknown. We have previously shown that the assembly of motor neurons into nuclei depends on cadherin-mediated adhesion. Here, we demonstrate that the emergence of mature topography among motor nuclei involves a novel interplay between spontaneous activity, cadherin expression and gap junction communication. We report that nuclei display spontaneous calcium transients, and that changes in the activity patterns coincide with the course of nucleogenesis. We also find that these activity patterns are disrupted by manipulating cadherin or gap junction expression. Furthermore, inhibition of activity disrupts nucleogenesis, suggesting that activity feeds back to maintain integrity among motor neurons within a nucleus. Our study suggests that a network of interactions between cadherins, gap junctions and spontaneous activity governs neuron assembly, presaging circuit formation.<\/jats:p>","DOI":"10.1242\/dev.144063","type":"journal-article","created":{"date-parts":[[2017,2,28]],"date-time":"2017-02-28T12:30:59Z","timestamp":1488285059000},"page":"830-836","source":"Crossref","is-referenced-by-count":9,"title":["The assembly of developing motor neurons depends on an interplay between spontaneous activity, type II cadherins and gap junctions"],"prefix":"10.1242","volume":"144","author":[{"given":"Karli","family":"Montague","sequence":"first","affiliation":[{"name":"Wolfson Centre for Age-related Diseases, King's College London, Guy's Hospital Campus, London SE1 1UL, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew S.","family":"Lowe","sequence":"additional","affiliation":[{"name":"Department of Developmental Neurobiology, King's College London, Guy's Hospital Campus, London SE1 1UL, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Uzquiano","sequence":"additional","affiliation":[{"name":"\u00c9cole de Neuroscience-Paris \u00cele-de-France, ENP-DIM, 15 Rue de L\u2019\u00c9cole de M\u00e9d\u00e9cine, Paris 75006, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Athene","family":"Kn\u00fcfer","sequence":"additional","affiliation":[{"name":"Department of Developmental Neurobiology, King's College London, Guy's Hospital Campus, London SE1 1UL, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Astick","sequence":"additional","affiliation":[{"name":"Institut de Recherche Interdisciplinaire en Biologie Humaine et Mol\u00e9culaire, Universit\u00e9 de Bruxelles, Route de Lennik 808, Bruxelles B1070, Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen R.","family":"Price","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology, University College London, Gower Street, London WC1E 6BT, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8446-9150","authenticated-orcid":false,"given":"Sarah","family":"Guthrie","sequence":"additional","affiliation":[{"name":"Department of Developmental Neurobiology, King's College London, Guy's Hospital Campus, London SE1 1UL, UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","reference":[{"key":"2021042609080434300_DEV144063C1","doi-asserted-by":"publisher","first-page":"2541","DOI":"10.1016\/j.cub.2014.08.067","article-title":"Central topography of cranial motor nuclei controlled by differential cadherin expression","volume":"24","author":"Astick","year":"2014","journal-title":"Curr. 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