{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T06:27:44Z","timestamp":1785738464538,"version":"3.56.0"},"reference-count":32,"publisher":"The Company of Biologists","issue":"1","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/501100000703","name":"Action on Hearing Loss","doi-asserted-by":"publisher","award":["PA13"],"award-info":[{"award-number":["PA13"]}],"id":[{"id":"10.13039\/501100000703","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100010269","name":"Welcome Trust","doi-asserted-by":"crossref","award":["102889\/Z\/13\/Z"],"award-info":[{"award-number":["102889\/Z\/13\/Z"]}],"id":[{"id":"10.13039\/100010269","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,1,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The middle ear epithelium is derived from neural crest and endoderm, which line distinct regions of the middle ear cavity. Here, we investigate the distribution of putative stem cell markers in the middle ear, combined with an analysis of the location of label-retaining cells (LRCs) to create a map of the middle ear mucosa. We show that proliferating cells and LRCs were associated with specific regions of the ear epithelium, concentrated in the hypotympanum at the base of the auditory bulla and around the ear drum. Sox2 was widely expressed in the endodermally derived ciliated pseudostratified epithelium of the hypotympanum. This part of the middle ear showed high levels of Wnt activity, as indicated by the expression of Axin2, a readout of Wnt signalling. Keratin 5 showed a more restricted expression within the basal cells of this region, with very little overlap between the Sox2- and keratin 5-positive epithelium, indicating that these genes mark distinct populations. Little expression of Sox2 or keratin 5 was observed in the neural crest-derived middle ear epithelium that lined the promontory, except in cases of otitis media when this epithelium underwent hyperplasia. This study lays the foundation for furthering our understanding of homeostasis and repair in the middle ear.<\/jats:p>","DOI":"10.1242\/dev.154393","type":"journal-article","created":{"date-parts":[[2018,1,12]],"date-time":"2018-01-12T01:20:25Z","timestamp":1515720025000},"source":"Crossref","is-referenced-by-count":19,"title":["Mapping the distribution of stem\/progenitor cells across the mouse middle ear during homeostasis and inflammation"],"prefix":"10.1242","volume":"145","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8871-6094","authenticated-orcid":false,"given":"Abigail S.","family":"Tucker","sequence":"first","affiliation":[{"name":"Centre for Craniofacial and Regenerative Biology 1 , Department of Craniofacial Development and Stem Cell Biology , , London SE1 9RT , UK"},{"name":"King's College London 1 , Department of Craniofacial Development and Stem Cell Biology , , London SE1 9RT , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlene J.","family":"Dyer","sequence":"additional","affiliation":[{"name":"Centre for Craniofacial and Regenerative Biology 1 , Department of Craniofacial Development and Stem Cell Biology , , London SE1 9RT , UK"},{"name":"King's College London 1 , Department of Craniofacial Development and Stem Cell Biology , , London SE1 9RT , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan M.","family":"Fons Romero","sequence":"additional","affiliation":[{"name":"Centre for Craniofacial and Regenerative Biology 1 , Department of Craniofacial Development and Stem Cell Biology , , London SE1 9RT , UK"},{"name":"King's College London 1 , Department of Craniofacial Development and Stem Cell Biology , , London SE1 9RT , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tathyane H. N.","family":"Teshima","sequence":"additional","affiliation":[{"name":"Centre for Craniofacial and Regenerative Biology 1 , Department of Craniofacial Development and Stem Cell Biology , , London SE1 9RT , UK"},{"name":"King's College London 1 , Department of Craniofacial Development and Stem Cell Biology , , London SE1 9RT , UK"},{"name":"School of Dentistry, University of S\u00e3o Paulo 2 Department of Stomatology , , S\u00e3o Paulo, 05508-000 , Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8701-1228","authenticated-orcid":false,"given":"Jennifer C.","family":"Fuchs","sequence":"additional","affiliation":[{"name":"Centre for Craniofacial and Regenerative Biology 1 , Department of Craniofacial Development and Stem Cell Biology , , London SE1 9RT , UK"},{"name":"King's College London 1 , Department of Craniofacial Development and Stem Cell Biology , , London SE1 9RT , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hannah","family":"Thompson","sequence":"additional","affiliation":[{"name":"Centre for Craniofacial and Regenerative Biology 1 , Department of Craniofacial Development and Stem Cell Biology , , London SE1 9RT , UK"},{"name":"King's College London 1 , Department of Craniofacial Development and Stem Cell Biology , , London SE1 9RT , UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2018,1,11]]},"reference":[{"key":"2024061216102368700_DEV154393C1","doi-asserted-by":"publisher","first-page":"433","DOI":"10.1016\/j.stem.2013.07.004","article-title":"Sox2+ stem\/progenitor cells in the adult mouse pituitary support organ homeostasis and have tumor-inducing potential","volume":"13","author":"Andoniadou","year":"2013","journal-title":"Cell Stem Cell"},{"key":"2024061216102368700_DEV154393C2","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1016\/j.stem.2011.09.001","article-title":"Sox2+ adult stem and progenitor cells are important for tissue regeneration and survival of mice","volume":"9","author":"Arnold","year":"2011","journal-title":"Cell Stem Cell"},{"key":"2024061216102368700_DEV154393C3","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1258\/002221504323086516","article-title":"Ciliary count in chronic suppurative otitis media: comparative quantitative study between mucosal and squamous types using scanning electron microscopy and image analysis","volume":"118","author":"Atef","year":"2004","journal-title":"J. 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