{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T09:16:23Z","timestamp":1790759783284,"version":"4.1.0"},"reference-count":24,"publisher":"The Company of Biologists","issue":"16","license":[{"start":{"date-parts":[[2018,2,15]],"date-time":"2018-02-15T00:00:00Z","timestamp":1518652800000},"content-version":"vor","delay-in-days":184,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/501100008982","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DGF 1106401"],"award-info":[{"award-number":["DGF 1106401"]}],"id":[{"id":"10.13039\/501100008982","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 GM074057"],"award-info":[{"award-number":["R01 GM074057"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,8,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>The blastema is a mass of progenitor cells responsible for regeneration of amputated salamander limbs and fish fins. Previous studies have indicated that resident cell sources producing the blastema contribute lineage-restricted progeny to regenerating tissue. However, these studies have labeled general cell types rather than granular cell subpopulations, and they do not explain the developmental transitions that must occur for distal structures to arise from cells with proximal identities in the appendage stump. Here, we find that regulatory sequences of tph1b, which encodes an enzyme that synthesizes serotonin, mark a subpopulation of fibroblast-like cells restricted to the joints of uninjured adult zebrafish fins. Amputation stimulates serotonin production in regenerating fin fibroblasts, yet targeted tph1b mutations abrogating this response do not disrupt fin regeneration. In uninjured animals, tph1b-expressing cells contribute fibroblast progeny that remain restricted to joints throughout life. By contrast, upon amputation, tph1b+ joint cells give rise to fibroblasts that distribute across the entire lengths of regenerating fin rays. Our experiments visualize and quantify how incorporation into an appendage blastema broadens the progeny contributions of a cellular subpopulation that normally has proximodistal restrictions.<\/jats:p>","DOI":"10.1242\/dev.155655","type":"journal-article","created":{"date-parts":[[2017,8,15]],"date-time":"2017-08-15T11:25:13Z","timestamp":1502796313000},"page":"2889-2895","source":"Crossref","is-referenced-by-count":29,"title":["Live fate-mapping of joint-associated fibroblasts visualizes expansion of cell contributions during zebrafish fin regeneration"],"prefix":"10.1242","volume":"144","author":[{"given":"Valerie A.","family":"Tornini","sequence":"first","affiliation":[{"name":"Duke University Medical Center 1 Department of Cell Biology , , Durham, NC 27710 , USA"},{"name":"Duke University 2 Regeneration Next , , Durham, NC 27710 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John D.","family":"Thompson","sequence":"additional","affiliation":[{"name":"Duke University Medical Center 1 Department of Cell Biology , , Durham, NC 27710 , USA"},{"name":"Duke University 2 Regeneration Next , , Durham, NC 27710 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond L.","family":"Allen","sequence":"additional","affiliation":[{"name":"Duke University Medical Center 1 Department of Cell Biology , , Durham, NC 27710 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6743-5709","authenticated-orcid":false,"given":"Kenneth D.","family":"Poss","sequence":"additional","affiliation":[{"name":"Duke University Medical Center 1 Department of Cell Biology , , Durham, NC 27710 , USA"},{"name":"Duke University 2 Regeneration Next , , Durham, NC 27710 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","reference":[{"key":"2024061213321609900_DEV155655C1","doi-asserted-by":"publisher","first-page":"924","DOI":"10.1016\/j.celrep.2015.09.050","article-title":"Serotonin promotes development and regeneration of spinal motor neurons in zebrafish","volume":"13","author":"Barreiro-Iglesias","year":"2015","journal-title":"Cell Rep."},{"key":"2024061213321609900_DEV155655C2","doi-asserted-by":"publisher","first-page":"355","DOI":"10.1146\/annurev.med.60.042307.110802","article-title":"The expanded biology of serotonin","volume":"60","author":"Berger","year":"2009","journal-title":"Annu. Rev. Med."},{"key":"2024061213321609900_DEV155655C3","doi-asserted-by":"publisher","first-page":"13525","DOI":"10.1073\/pnas.2233056100","article-title":"Disruption of the nonneuronal tph1 gene demonstrates the importance of peripheral serotonin in cardiac function","volume":"100","author":"Cote","year":"2003","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2024061213321609900_DEV155655C4","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1007\/s10158-012-0140-y","article-title":"Serotonin circuits and anxiety: what can invertebrates teach us?","volume":"12","author":"Curran","year":"2012","journal-title":"Invert. Neurosci."},{"key":"2024061213321609900_DEV155655C5","doi-asserted-by":"crossref","first-page":"1321","DOI":"10.1093\/genetics\/155.3.1321","article-title":"Genetic analysis of isometric growth control mechanisms in the zebrafish caudal Fin","volume":"155","author":"Iovine","year":"2000","journal-title":"Genetics"},{"key":"2024061213321609900_DEV155655C6","doi-asserted-by":"publisher","first-page":"713","DOI":"10.1016\/j.devcel.2011.04.014","article-title":"Bone regenerates via dedifferentiation of osteoblasts in the zebrafish fin","volume":"20","author":"Knopf","year":"2011","journal-title":"Dev. Cell"},{"key":"2024061213321609900_DEV155655C7","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1038\/nature08152","article-title":"Cells keep a memory of their tissue origin during axolotl limb regeneration","volume":"460","author":"Kragl","year":"2009","journal-title":"Nature"},{"key":"2024061213321609900_DEV155655C8","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1006\/dbio.2002.0711","article-title":"In vivo imaging of embryonic vascular development using transgenic zebrafish","volume":"248","author":"Lawson","year":"2002","journal-title":"Dev. Biol."},{"key":"2024061213321609900_DEV155655C9","doi-asserted-by":"publisher","first-page":"20609","DOI":"10.1073\/pnas.1118017108","article-title":"Mouse digit tip regeneration is mediated by fate-restricted progenitor cells","volume":"108","author":"Lehoczky","year":"2011","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2024061213321609900_DEV155655C10","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1126\/science.1123842","article-title":"Platelet-derived serotonin mediates liver regeneration","volume":"312","author":"Lesurtel","year":"2006","journal-title":"Science"},{"key":"2024061213321609900_DEV155655C11","doi-asserted-by":"crossref","first-page":"5407","DOI":"10.1523\/JNEUROSCI.13-12-05407.1993","article-title":"Serotonin-deficient mutants and male mating behavior in the nematode Caenorhabditis elegans","volume":"13","author":"Loer","year":"1993","journal-title":"J. Neurosci."},{"key":"2024061213321609900_DEV155655C12","doi-asserted-by":"publisher","first-page":"169","DOI":"10.1242\/dev.059345","article-title":"Ubiquitous transgene expression and Cre-based recombination driven by the ubiquitin promoter in zebrafish","volume":"138","author":"Mosimann","year":"2011","journal-title":"Development"},{"key":"2024061213321609900_DEV155655C13","doi-asserted-by":"publisher","first-page":"3754","DOI":"10.1242\/dev.098798","article-title":"Transcriptional components of anteroposterior positional information during zebrafish fin regeneration","volume":"140","author":"Nachtrab","year":"2013","journal-title":"Development"},{"key":"2024061213321609900_DEV155655C14","doi-asserted-by":"publisher","first-page":"756","DOI":"10.1111\/j.1601-183X.2007.00307.x","article-title":"Compartmentalization of neuronal and peripheral serotonin synthesis in Drosophila melanogaster","volume":"6","author":"Neckameyer","year":"2007","journal-title":"Genes Brain Behav."},{"key":"2024061213321609900_DEV155655C15","doi-asserted-by":"publisher","first-page":"409","DOI":"10.1038\/nature10346","article-title":"Germ-layer and lineage-restricted stem\/progenitors regenerate the mouse digit tip","volume":"476","author":"Rinkevich","year":"2011","journal-title":"Nature"},{"key":"2024061213321609900_DEV155655C16","doi-asserted-by":"publisher","first-page":"1240","DOI":"10.1002\/dvdy.22534","article-title":"Evx1 is required for joint formation in zebrafish fin dermoskeleton","volume":"240","author":"Schulte","year":"2011","journal-title":"Dev. Dyn."},{"key":"2024061213321609900_DEV155655C17","doi-asserted-by":"publisher","first-page":"879","DOI":"10.1016\/j.devcel.2012.03.006","article-title":"Regeneration of amputated zebrafish fin rays from de novo osteoblasts","volume":"22","author":"Singh","year":"2012","journal-title":"Dev. Cell"},{"key":"2024061213321609900_DEV155655C18","doi-asserted-by":"publisher","first-page":"533","DOI":"10.1016\/j.cmet.2008.04.012","article-title":"Serotonin regulates C. elegans fat and feeding through independent molecular mechanisms","volume":"7","author":"Srinivasan","year":"2008","journal-title":"Cell Metab."},{"key":"2024061213321609900_DEV155655C19","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1016\/j.ydbio.2012.02.031","article-title":"Limited dedifferentiation provides replacement tissue during zebrafish fin regeneration","volume":"365","author":"Stewart","year":"2012","journal-title":"Dev. Biol."},{"key":"2024061213321609900_DEV155655C20","doi-asserted-by":"publisher","first-page":"560","DOI":"10.1038\/35000609","article-title":"Food and metabolic signalling defects in a Caenorhabditis elegans serotonin-synthesis mutant","volume":"403","author":"Sze","year":"2000","journal-title":"Nature"},{"key":"2024061213321609900_DEV155655C21","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1016\/j.devcel.2014.04.007","article-title":"Keeping at arm's length during regeneration","volume":"29","author":"Tornini","year":"2014","journal-title":"Dev. Cell"},{"key":"2024061213321609900_DEV155655C22","doi-asserted-by":"publisher","first-page":"2981","DOI":"10.1016\/j.cub.2016.08.072","article-title":"Live monitoring of blastemal cell contributions during appendage regeneration","volume":"26","author":"Tornini","year":"2016","journal-title":"Curr. Biol."},{"key":"2024061213321609900_DEV155655C23","doi-asserted-by":"publisher","first-page":"725","DOI":"10.1016\/j.devcel.2011.04.013","article-title":"Fate restriction in the growing and regenerating zebrafish fin","volume":"20","author":"Tu","year":"2011","journal-title":"Dev. Cell"},{"key":"2024061213321609900_DEV155655C24","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1016\/S0896-6273(00)80527-9","article-title":"Control of alternative behavioral states by serotonin in Caenorhabditis elegans","volume":"21","author":"Waggoner","year":"1998","journal-title":"Neuron"}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1242\/dev.155655","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/144\/16\/2889\/3473312\/dev155655.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/journals.biologists.com\/dev\/article-pdf\/144\/16\/2889\/3473312\/dev155655.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,12]],"date-time":"2024-06-12T13:37:58Z","timestamp":1718199478000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/144\/16\/2889\/19215\/Live-fate-mapping-of-joint-associated-fibroblasts"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,8,15]]},"references-count":24,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2017,8,15]]}},"URL":"https:\/\/doi.org\/10.1242\/dev.155655","relation":{},"ISSN":["1477-9129","0950-1991"],"issn-type":[{"value":"1477-9129","type":"electronic"},{"value":"0950-1991","type":"print"}],"subject":[],"published":{"date-parts":[[2017,8,15]]}}}