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While the molecular mechanism that regulates transient cartilage differentiation is relatively well understood, the mechanism of articular cartilage differentiation has only begun to be unraveled. Furthermore, the molecules that coordinate the articular and transient cartilage differentiation processes are poorly understood. Here, we have characterized in chick the regulatory roles of two transcription factors, NFIA and GATA3, in articular cartilage differentiation, maintenance and the coordinated differentiation of articular and transient cartilage. Both NFIA and GATA3 block hypertrophic differentiation. Our results suggest that NFIA is not sufficient but necessary for articular cartilage differentiation. Ectopic activation of GATA3 promotes articular cartilage differentiation, whereas inhibition of GATA3 activity promotes transient cartilage differentiation at the expense of articular cartilage. We propose a novel transcriptional circuitry involved in embryonic articular cartilage differentiation, maintenance and its crosstalk with the transient cartilage differentiation program.<\/jats:p>","DOI":"10.1242\/dev.156554","type":"journal-article","created":{"date-parts":[[2018,10,8]],"date-time":"2018-10-08T12:46:02Z","timestamp":1539002762000},"source":"Crossref","is-referenced-by-count":27,"title":["NFIA and GATA3 are crucial regulators of embryonic articular cartilage differentiation"],"prefix":"10.1242","volume":"145","author":[{"given":"Pratik Narendra Pratap","family":"Singh","sequence":"first","affiliation":[{"name":"Indian Institute of Technology 1 Department of Biological Sciences and Bioengineering , , Kanpur, U.P . 208016 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Upendra Singh","family":"Yadav","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology 1 Department of Biological Sciences and Bioengineering , , Kanpur, U.P . 208016 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimi","family":"Azad","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology 1 Department of Biological Sciences and Bioengineering , , Kanpur, U.P . 208016 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3900-6947","authenticated-orcid":false,"given":"Pooja","family":"Goswami","sequence":"additional","affiliation":[{"name":"Kalinga Institute of Industrial Technology, KIIT University 2 , Bhubaneswar 751024, Odisha , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5089-5151","authenticated-orcid":false,"given":"Veena","family":"Kinare","sequence":"additional","affiliation":[{"name":"Sophia College for Women 3 Department of Lifesciences , , Bhulabhai Desai Road, Mumbai 400026 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0429-438X","authenticated-orcid":false,"given":"Amitabha","family":"Bandyopadhyay","sequence":"additional","affiliation":[{"name":"Indian Institute of Technology 1 Department of Biological Sciences and Bioengineering , , Kanpur, U.P . 208016 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2018,1,17]]},"reference":[{"key":"2024061223452292600_DEV156554C1","doi-asserted-by":"publisher","first-page":"2235","DOI":"10.1128\/MCB.13.4.2235","article-title":"Mouse GATA-4: a retinoic acid-inducible GATA-binding transcription factor expressed in endodermally derived tissues and heart","volume":"13","author":"Arceci","year":"1993","journal-title":"Mol. 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