{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:50:00Z","timestamp":1792788600461,"version":"4.5.0"},"reference-count":38,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2017,1,1]],"date-time":"2017-01-01T00:00:00Z","timestamp":1483228800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"name":"National Institute of Health","award":["R00HD073269"],"award-info":[{"award-number":["R00HD073269"]}]},{"name":"National Institute of Health","award":["R01NS097231"],"award-info":[{"award-number":["R01NS097231"]}]},{"name":"National Institute of Health","award":["R01NS096176"],"award-info":[{"award-number":["R01NS096176"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>The Zika virus (ZIKV) has two lineages, Asian and African, and their impact on developing brains has not been compared. Dengue virus (DENV) is a close family member of ZIKV and co-circulates with ZIKV. Here we performed intracerebral inoculation of embryonic mouse brains with dengue virus 2 (DENV2), and found that DENV2 is sufficient to cause smaller brain size due to increased cell death in neural progenitor cells (NPCs) and neurons. Compared to the currently circulating Asian lineage of ZIKV (MEX1-44), DENV2 grows slower, causes less neuronal death, and fails to cause postnatal animal death. Surprisingly, our side-by-side comparison uncovered that African ZIKV isolate (MR-766) is more potent in causing brain damage and postnatal lethality than MEX1-44. In comparison to MEX1-44, MR-766 grows faster in NPCs and in the developing brain, and causes more pronounced cell death in NPCs and neurons, resulting in more severe neuronal loss. Together, these results reveal that DENV2 is sufficient to cause smaller brain sizes, and suggest that the ZIKV African lineage is more virulent and causes more severe brain damage than the Asian lineage.<\/jats:p>","DOI":"10.1242\/dev.156752","type":"journal-article","created":{"date-parts":[[2017,10,9]],"date-time":"2017-10-09T20:10:32Z","timestamp":1507579832000},"source":"Crossref","is-referenced-by-count":61,"title":["The African Zika virus MR-766 is more virulent and causes more severe brain damage than current Asian lineage and Dengue virus"],"prefix":"10.1242","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5307-8264","authenticated-orcid":false,"given":"Qiang","family":"Shao","sequence":"first","affiliation":[{"name":"Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, CA 90033, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6049-0246","authenticated-orcid":false,"given":"Stephanie","family":"Herrlinger","sequence":"additional","affiliation":[{"name":"Department of Genetics, Department of Biochemistry & Molecular Biology, University of Georgia, Athens, GA, 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Nan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Genetics, Department of Biochemistry & Molecular Biology, University of Georgia, Athens, GA, 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9988-8991","authenticated-orcid":false,"given":"Mei","family":"Yang","sequence":"additional","affiliation":[{"name":"Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, CA 90033, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3717-9433","authenticated-orcid":false,"given":"Forrest","family":"Goodfellow","sequence":"additional","affiliation":[{"name":"Regenerative Bioscience Center, University of Georgia, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5710-5872","authenticated-orcid":false,"given":"Steven L.","family":"Stice","sequence":"additional","affiliation":[{"name":"Regenerative Bioscience Center, University of Georgia, Athens, GA 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7982-7486","authenticated-orcid":false,"given":"Xiao-Peng","family":"Qi","sequence":"additional","affiliation":[{"name":"Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4929-8085","authenticated-orcid":false,"given":"Melinda A.","family":"Brindley","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, Department of Population Health and Center for Vaccines and Immunology, University of Georgia, Athens, GA, 30602, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8869-5913","authenticated-orcid":false,"given":"Jian-Fu","family":"Chen","sequence":"additional","affiliation":[{"name":"Center for Craniofacial Molecular Biology, University of Southern California, Los Angeles, CA 90033, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2017,1,1]]},"reference":[{"key":"2021042609045337100_DEV156752C1","doi-asserted-by":"publisher","first-page":"766","DOI":"10.1038\/192766b0","article-title":"Autoradiographic study of cell migration during histogenesis of cerebral cortex in the mouse","volume":"192","author":"Angevine","year":"1961","journal-title":"Nature"},{"key":"2021042609045337100_DEV156752C2","doi-asserted-by":"publisher","first-page":"39384","DOI":"10.1038\/srep39384","article-title":"Simple reverse genetics systems for Asian and African Zika viruses","volume":"6","author":"Atieh","year":"2016","journal-title":"Sci. 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