{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T14:15:25Z","timestamp":1790777725818,"version":"4.1.0"},"reference-count":29,"publisher":"The Company of Biologists","issue":"2","license":[{"start":{"date-parts":[[2018,1,15]],"date-time":"2018-01-15T00:00:00Z","timestamp":1515974400000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/100000050","name":"National Heart, Lung and Blood Institute","doi-asserted-by":"crossref","award":["R01HL091724"],"award-info":[{"award-number":["R01HL091724"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung and Blood Institute","doi-asserted-by":"crossref","award":["U01HL100405"],"award-info":[{"award-number":["U01HL100405"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000050","name":"National Heart, Lung and Blood Institute","doi-asserted-by":"crossref","award":["1F31HL120615"],"award-info":[{"award-number":["1F31HL120615"]}],"id":[{"id":"10.13039\/100000050","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["T32 DK07780"],"award-info":[{"award-number":["T32 DK07780"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["T32CA09140"],"award-info":[{"award-number":["T32CA09140"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100005189","name":"Leukemia and Lymphoma Society","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005189","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2018,1,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n               <jats:p>Hematopoietic cells differentiate during embryogenesis from a population of endothelial cells called hemogenic endothelium (HE) in a process called the endothelial-to-hematopoietic transition (EHT). The transcription factor Runx1 is required for EHT, but for how long and which endothelial cells are competent to respond to Runx1 are not known. Here, we show that the ability of Runx1 to induce EHT in non-hemogenic endothelial cells depends on the anatomical location of the cell and the developmental age of the conceptus. Ectopic expression of Runx1 in non-hemogenic endothelial cells between embryonic day (E) 7.5 and E8.5 promoted the formation of erythro-myeloid progenitors (EMPs) specifically in the yolk sac, the dorsal aorta and the heart. The increase in EMPs was accompanied by a higher frequency of HE cells able to differentiate into EMPs in vitro. Expression of Runx1 just 1 day later (E8.5-E9.5) failed to induce the ectopic formation of EMPs. Therefore, endothelial cells, located in specific sites in the conceptus, have a short developmental window of competency during which they can respond to Runx1 and differentiate into blood cells.<\/jats:p>","DOI":"10.1242\/dev.158162","type":"journal-article","created":{"date-parts":[[2018,1,19]],"date-time":"2018-01-19T01:20:13Z","timestamp":1516324813000},"source":"Crossref","is-referenced-by-count":53,"title":["Runx1 is sufficient for blood cell formation from non-hemogenic endothelial cells <i>in vivo<\/i> only during early embryogenesis"],"prefix":"10.1242","volume":"145","author":[{"given":"Amanda D.","family":"Yzaguirre","sequence":"first","affiliation":[{"name":"Abramson Family Cancer Research Institute 1 , Department of Cell and Developmental Biology , , Philadelphia, PA 19104 , USA"},{"name":"Institute for Regenerative Medicine, Perelman School of Medicine at the University of Pennsylvania 1 , Department of Cell and Developmental Biology , , Philadelphia, PA 19104 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth D.","family":"Howell","sequence":"additional","affiliation":[{"name":"Abramson Family Cancer Research Institute 1 , Department of Cell and Developmental Biology , , Philadelphia, PA 19104 , USA"},{"name":"Institute for Regenerative Medicine, Perelman School of Medicine at the University of Pennsylvania 1 , Department of Cell and Developmental Biology , , Philadelphia, PA 19104 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Li","sequence":"additional","affiliation":[{"name":"Abramson Family Cancer Research Institute 1 , Department of Cell and Developmental Biology , , Philadelphia, PA 19104 , USA"},{"name":"Institute for Regenerative Medicine, Perelman School of Medicine at the University of Pennsylvania 1 , Department of Cell and Developmental Biology , , Philadelphia, PA 19104 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zijing","family":"Liu","sequence":"additional","affiliation":[{"name":"Beijing Institute of Biotechnology 2 , Beijing 100850 , People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1893-582X","authenticated-orcid":false,"given":"Nancy A.","family":"Speck","sequence":"additional","affiliation":[{"name":"Abramson Family Cancer Research Institute 1 , Department of Cell and Developmental Biology , , Philadelphia, PA 19104 , USA"},{"name":"Institute for Regenerative Medicine, Perelman School of Medicine at the University of Pennsylvania 1 , Department of Cell and Developmental Biology , , Philadelphia, PA 19104 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2018,1,29]]},"reference":[{"key":"2024061223473743200_DEV158162C1","doi-asserted-by":"publisher","first-page":"889","DOI":"10.1038\/nature07619","article-title":"Runx1 is required for the endothelial to haematopoietic cell transition but not thereafter","volume":"457","author":"Chen","year":"2009","journal-title":"Nature"},{"key":"2024061223473743200_DEV158162C2","doi-asserted-by":"publisher","first-page":"937","DOI":"10.1038\/nature04479","article-title":"Endothelial cells and VEGF in vascular development","volume":"438","author":"Coultas","year":"2005","journal-title":"Nature"},{"key":"2024061223473743200_DEV158162C3","doi-asserted-by":"publisher","first-page":"2185","DOI":"10.1016\/j.celrep.2016.05.001","article-title":"The hemogenic competence of endothelial progenitors is restricted by Runx1 silencing during embryonic development","volume":"15","author":"Eliades","year":"2016","journal-title":"Cell Rep."},{"key":"2024061223473743200_DEV158162C4","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1002\/stem.2213","article-title":"Definitive hematopoiesis in the Yolk Sac emerges from Wnt-responsive hemogenic endothelium independently of circulation and arterial identity","volume":"34","author":"Frame","year":"2015","journal-title":"Stem Cells"},{"key":"2024061223473743200_DEV158162C5","doi-asserted-by":"publisher","first-page":"3194","DOI":"10.1182\/blood-2008-02-139055","article-title":"Cell signaling directing the formation and function of hemogenic endothelium during murine embryogenesis","volume":"112","author":"Goldie","year":"2008","journal-title":"Blood"},{"key":"2024061223473743200_DEV158162C6","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1016\/j.jim.2009.06.008","article-title":"ELDA: extreme limiting dilution analysis for comparing depleted and enriched populations in stem cell and other assays","volume":"347","author":"Hu","year":"2009","journal-title":"J. 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