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The transcription factor Shox2 (short stature homeobox\u00a02) is involved in the generation of TRKB+ low-threshold mechanoreceptors (LTMRs), but mechanisms terminating this program and allowing alternative fates are unknown. Here, we show that the conditional loss of the miR-183-96-182 cluster in mouse leads to a failure of extinction of Shox2 during development and an increase in the proportion of A\u03b4 LTMRs (TRKB+\/NECAB2+) neurons at the expense of A\u03b2 slowly adapting (SA)-LTMRs (TRKC+\/Runx3\u2212) neurons. Conversely, overexpression of miR-183 cluster that represses Shox2 expression, or loss of Shox2, both increase the A\u03b2 SA-LTMRs population at the expense of A\u03b4 LTMRs. Our results suggest that the miR-183 cluster determines the timing of Shox2 expression by direct targeting during development, and through this determines the population sizes of A\u03b4 LTMRs and A\u03b2 SA-LTMRs.<\/jats:p>","DOI":"10.1242\/dev.165613","type":"journal-article","created":{"date-parts":[[2018,8,20]],"date-time":"2018-08-20T11:35:22Z","timestamp":1534764922000},"source":"Crossref","is-referenced-by-count":11,"title":["Termination of cell-type specification gene programs by the miR-183 cluster determines the population sizes of low-threshold mechanosensitive neurons"],"prefix":"10.1242","volume":"145","author":[{"given":"Changgeng","family":"Peng","sequence":"first","affiliation":[{"name":"The First Rehabilitation Hospital of Shanghai, Tongji University School of Medicine 1 , 200029 Shanghai , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandro","family":"Furlan","sequence":"additional","affiliation":[{"name":"Karolinska Institutet 2 Department of Medical Biochemistry and Biophysics, Division of Molecular Neurobiology , , 17177 Stockholm , Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming-Dong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Karolinska Institutet 2 Department of Medical Biochemistry and Biophysics, Division of Molecular Neurobiology , , 17177 Stockholm , Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Su","sequence":"additional","affiliation":[{"name":"Karolinska Institutet 2 Department of Medical Biochemistry and Biophysics, Division of Molecular Neurobiology , , 17177 Stockholm , Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8103-0185","authenticated-orcid":false,"given":"Moritz","family":"L\u00fcbke","sequence":"additional","affiliation":[{"name":"Karolinska Institutet 2 Department of Medical Biochemistry and Biophysics, Division of Molecular Neurobiology , , 17177 Stockholm , Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"L\u00f6nnerberg","sequence":"additional","affiliation":[{"name":"Karolinska Institutet 2 Department of Medical Biochemistry and Biophysics, Division of Molecular Neurobiology , , 17177 Stockholm , Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hind","family":"Abdo","sequence":"additional","affiliation":[{"name":"Karolinska Institutet 2 Department of Medical Biochemistry and Biophysics, Division of Molecular Neurobiology , , 17177 Stockholm , Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jana","family":"Sontheimer","sequence":"additional","affiliation":[{"name":"Karolinska Institutet 2 Department of Medical Biochemistry and Biophysics, Division of Molecular Neurobiology , , 17177 Stockholm , Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erik","family":"Sundstr\u00f6m","sequence":"additional","affiliation":[{"name":"Care Sciences and Society, Karolinska Institutet 3 Department of Neurobiology , , 17177 Stockholm , Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1140-3986","authenticated-orcid":false,"given":"Patrik","family":"Ernfors","sequence":"additional","affiliation":[{"name":"Karolinska Institutet 2 Department of Medical Biochemistry and Biophysics, Division of Molecular Neurobiology , , 17177 Stockholm , Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"237","published-online":{"date-parts":[[2018,9,25]]},"reference":[{"key":"2024061300162952800_DEV165613C1","doi-asserted-by":"publisher","first-page":"1529","DOI":"10.1111\/j.1460-9568.2011.07883.x","article-title":"Dependence on the transcription factor Shox2 for specification of sensory neurons conveying discriminative touch","volume":"34","author":"Abdo","year":"2011","journal-title":"Eur. 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