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Integration of internal cues, such as signalling pathways, with external cues such as nutritional status is paramount for an orderly progression of development in function of growth. In Drosophila, this involves insulin and steroid signalling, but the underlying mechanisms and their coordination are incompletely understood. We show that bioactive 20-hydroxyecdysone production by the enzyme Shade in the fat body is a nutrient-dependent process. We demonstrate that under fed conditions, Shade plays a role in growth control. We identify the trachea and the insulin-producing cells in the brain as direct targets through which 20-hydroxyecdysone regulates insulin-signaling. The identification of the trachea-dependent regulation of insulin-signaling exposes an important variable that may have been overlooked in other studies focusing on insulin-signaling in Drosophila. Our findings provide a potentially conserved, novel mechanism by which nutrition can modulate steroid hormone bioactivation, reveal an important caveat of a commonly used transgenic tool to study IPC function and yield further insights as to how steroid and insulin signalling are coordinated during development to regulate growth and developmental timing.<\/jats:p>","DOI":"10.1242\/dev.165654","type":"journal-article","created":{"date-parts":[[2018,9,28]],"date-time":"2018-09-28T07:39:23Z","timestamp":1538120363000},"source":"Crossref","is-referenced-by-count":35,"title":["Growth control through regulation of insulin-signaling by nutrition-activated steroid hormone in\n                    <i>Drosophila<\/i>"],"prefix":"10.1242","author":[{"given":"Kurt","family":"Buhler","sequence":"first","affiliation":[{"name":"Laboratory of Behavioral and Developmental Genetics, Department of Human Genetics, KU Leuven - University of Leuven, Herestraat 49, Box 602, B-3000, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"Clements","sequence":"additional","affiliation":[{"name":"Laboratory of Behavioral and Developmental Genetics, Department of Human Genetics, KU Leuven - University of Leuven, Herestraat 49, Box 602, B-3000, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mattias","family":"Winant","sequence":"additional","affiliation":[{"name":"Laboratory of Behavioral and Developmental Genetics, Department of Human Genetics, KU Leuven - University of Leuven, Herestraat 49, Box 602, B-3000, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lenz","family":"Bolckmans","sequence":"additional","affiliation":[{"name":"Laboratory of Behavioral and Developmental Genetics, Department of Human Genetics, KU Leuven - University of Leuven, Herestraat 49, Box 602, B-3000, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veerle","family":"Vulsteke","sequence":"additional","affiliation":[{"name":"Laboratory of Behavioral and Developmental Genetics, Department of Human Genetics, KU Leuven - University of Leuven, Herestraat 49, Box 602, B-3000, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4530-4561","authenticated-orcid":false,"given":"Patrick","family":"Callaerts","sequence":"additional","affiliation":[{"name":"Laboratory of Behavioral and Developmental Genetics, Department of Human Genetics, KU Leuven - University of Leuven, Herestraat 49, Box 602, B-3000, Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2018,1,1]]},"reference":[{"key":"2021042609155786700_DEV165654C1","doi-asserted-by":"publisher","first-page":"675","DOI":"10.1016\/j.cmet.2016.03.003","article-title":"The Drosophila TNF eiger is an adipokine that acts on insulin-producing cells to mediate nutrient response","volume":"23","author":"Agrawal","year":"2016","journal-title":"Cell Metab."},{"key":"2021042609155786700_DEV165654C2","doi-asserted-by":"publisher","first-page":"2410","DOI":"10.1242\/dev.133595","article-title":"A biological timer in the fat body comprising Blimp-1, BFtz-f1 and Shade regulates pupation timing in Drosophila melanogaster","volume":"143","author":"Akagi","year":"2016","journal-title":"Development"},{"key":"2021042609155786700_DEV165654C3","doi-asserted-by":"publisher","first-page":"978","DOI":"10.1111\/acel.12000","article-title":"Drosophila insulin-like peptide-6 (dilp6) expression from fat body extends lifespan and represses secretion of Drosophila insulin-like peptide-2 from the brain","volume-title":"Aging Cell","author":"Bai","year":"2012"},{"key":"2021042609155786700_DEV165654C4","doi-asserted-by":"publisher","first-page":"731","DOI":"10.1016\/S0092-8674(03)00420-3","article-title":"The Drosophila orphan nuclear receptor DHR38 mediates an atypical ecdysteroid signaling pathway","volume":"113","author":"Baker","year":"2003","journal-title":"Cell"},{"key":"2021042609155786700_DEV165654C5","doi-asserted-by":"publisher","first-page":"570","DOI":"10.1016\/j.ibmb.2007.03.001","article-title":"Specific transcriptional responses to juvenile hormone and ecdysone in Drosophila","volume":"37","author":"Beckstead","year":"2007","journal-title":"Insect Biochem. 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