{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T20:07:09Z","timestamp":1785874029490,"version":"3.56.0"},"reference-count":96,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2018,1,1]],"date-time":"2018-01-01T00:00:00Z","timestamp":1514764800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/501100004359","name":"Vetenskapsr?det","doi-asserted-by":"publisher","award":["621-2003-3408"],"award-info":[{"award-number":["621-2003-3408"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002794","name":"Cancerfonden","doi-asserted-by":"publisher","award":["4714-B03-02XBB"],"award-info":[{"award-number":["4714-B03-02XBB"]}],"id":[{"id":"10.13039\/501100002794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006313","name":"Barncancerfonden","doi-asserted-by":"publisher","award":["2015\/0096"],"award-info":[{"award-number":["2015\/0096"]}],"id":[{"id":"10.13039\/501100006313","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"crossref","award":["Ho-2559\/3-3"],"award-info":[{"award-number":["Ho-2559\/3-3"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Basement membranes (BMs) are specialized layers of extracellular matrix (ECM) mainly composed of Laminin, type IV Collagen, Perlecan and Nidogen\/entactin (NDG). Recent in vivo studies challenged the initially proposed role of NDG as major ECM linker molecule by revealing dispensability for viability and BM formation. Here, we report the characterization of the single Ndg gene in Drosophila. Embryonic Ndg expression was primarily observed in mesodermal tissues and the chordotonal organs, whereas NDG protein localized to all BMs. While loss of Laminin strongly affected BM-localization of NDG, Ndg null mutants exhibited no overt changes in the distribution of BM components. Although Drosophila Ndg mutants were viable, loss of NDG led to ultrastructural BM defects compromising barrier function and stability in vivo. Moreover, loss of NDG impaired larval crawling behavior and reduced responses to vibrational stimuli. Further morphological analysis revealed accompanying defects in the larval peripheral nervous system especially in the chordotonal organs and the neuromuscular junction (NMJ). Taken together, our analysis suggests that NDG is not essential for BM assembly but mediates BM stability and ECM-dependent neural plasticity during Drosophila development.<\/jats:p>","DOI":"10.1242\/dev.168948","type":"journal-article","created":{"date-parts":[[2018,12,19]],"date-time":"2018-12-19T10:55:24Z","timestamp":1545216924000},"source":"Crossref","is-referenced-by-count":20,"title":["Characterization of\n                    <i>Drosophila Nidogen<\/i>\n                    \/\n                    <i>entactin<\/i>\n                    reveals roles in basement membrane stability, barrier function and nervous system patterning"],"prefix":"10.1242","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8763-1419","authenticated-orcid":false,"given":"Georg","family":"Wolfstetter","sequence":"first","affiliation":[{"name":"Justus-Liebig-Universitaet Giessen, Institut f\u00fcr Allgemeine und Spezielle Zoologie, Allgemeine Zoologie und Entwicklungsbiologie, Stephanstra\u00dfe 24, 35390 Gie\u00dfen, Germany"},{"name":"The Sahlgrenska Academy at the University of Gothenburg, Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology, Medicinaregatan 9A, 41390 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ina","family":"Dahlitz","sequence":"additional","affiliation":[{"name":"Justus-Liebig-Universitaet Giessen, Institut f\u00fcr Allgemeine und Spezielle Zoologie, Allgemeine Zoologie und Entwicklungsbiologie, Stephanstra\u00dfe 24, 35390 Gie\u00dfen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2680-8425","authenticated-orcid":false,"given":"Kathrin","family":"Pfeifer","sequence":"additional","affiliation":[{"name":"The Sahlgrenska Academy at the University of Gothenburg, Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology, Medicinaregatan 9A, 41390 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Uwe","family":"T\u00f6pfer","sequence":"additional","affiliation":[{"name":"Justus-Liebig-Universitaet Giessen, Institut f\u00fcr Allgemeine und Spezielle Zoologie, Allgemeine Zoologie und Entwicklungsbiologie, Stephanstra\u00dfe 24, 35390 Gie\u00dfen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9144-1041","authenticated-orcid":false,"given":"Joscha Arne","family":"Alt","sequence":"additional","affiliation":[{"name":"Justus-Liebig-Universitaet Giessen, Institut f\u00fcr Tierphysiologie, Integrative Sinnesphysiologie, Heinrich-Buff-Ring 26, 35392 Gie\u00dfen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7163-9985","authenticated-orcid":false,"given":"Daniel Christoph","family":"Pfeifer","sequence":"additional","affiliation":[{"name":"Justus-Liebig-Universitaet Giessen, Institut f\u00fcr Allgemeine und Spezielle Zoologie, Allgemeine Zoologie und Entwicklungsbiologie, Stephanstra\u00dfe 24, 35390 Gie\u00dfen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinhard","family":"Lakes-Harlan","sequence":"additional","affiliation":[{"name":"Justus-Liebig-Universitaet Giessen, Institut f\u00fcr Tierphysiologie, Integrative Sinnesphysiologie, Heinrich-Buff-Ring 26, 35392 Gie\u00dfen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5320-8321","authenticated-orcid":false,"given":"Stefan","family":"Baumgartner","sequence":"additional","affiliation":[{"name":"Lund University, Department of Experimental Medical Sciences, BMC D10, 22184 Lund, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2735-8470","authenticated-orcid":false,"given":"Ruth H.","family":"Palmer","sequence":"additional","affiliation":[{"name":"The Sahlgrenska Academy at the University of Gothenburg, Institute of Biomedicine, Department of Medical Biochemistry and Cell Biology, Medicinaregatan 9A, 41390 Gothenburg, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7028-6548","authenticated-orcid":false,"given":"Anne","family":"Holz","sequence":"additional","affiliation":[{"name":"Justus-Liebig-Universitaet Giessen, Institut f\u00fcr Allgemeine und Spezielle Zoologie, Allgemeine Zoologie und Entwicklungsbiologie, Stephanstra\u00dfe 24, 35390 Gie\u00dfen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2018,1,1]]},"reference":[{"key":"2021042513255604900_DEV168948C1","doi-asserted-by":"publisher","first-page":"3577","DOI":"10.1523\/JNEUROSCI.23-09-03577.2003","article-title":"The basement membrane components nidogen and type XVIII collagen regulate organization of neuromuscular junctions in Caenorhabditis elegans","volume":"23","author":"Ackley","year":"2003","journal-title":"J. Neurosci."},{"key":"2021042513255604900_DEV168948C2","doi-asserted-by":"publisher","first-page":"1091","DOI":"10.1534\/genetics.113.156323","article-title":"The extracellular matrix protein artichoke is required for integrity of ciliated mechanosensory and chemosensory organs in Drosophila embryos","volume":"196","author":"Andres","year":"2014","journal-title":"Genetics"},{"key":"2021042513255604900_DEV168948C3","doi-asserted-by":"publisher","first-page":"354","DOI":"10.1038\/15537","article-title":"Perlecan is essential for cartilage and cephalic development","volume":"23","author":"Arikawa-Hirasawa","year":"1999","journal-title":"Nat. Genet."},{"key":"2021042513255604900_DEV168948C4","doi-asserted-by":"publisher","first-page":"6257","DOI":"10.1242\/dev.00843","article-title":"Notch and Ras signaling pathway effector genes expressed in fusion competent and founder cells during Drosophila myogenesis","volume":"130","author":"Artero","year":"2003","journal-title":"Development"},{"key":"2021042513255604900_DEV168948C5","volume-title":"Drosophila: A Laboratory Manual","author":"Ashburner","year":"1989"},{"key":"2021042513255604900_DEV168948C6","doi-asserted-by":"publisher","first-page":"7","DOI":"10.1038\/ki.1993.3","article-title":"Nidogen mediates the formation of ternary complexes of basement membrane components","volume":"43","author":"Aumailley","year":"1993","journal-title":"Kidney Int."},{"key":"2021042513255604900_DEV168948C7","doi-asserted-by":"publisher","first-page":"6846","DOI":"10.1128\/MCB.25.15.6846-6856.2005","article-title":"Compound genetic ablation of nidogen 1 and 2 causes basement membrane defects and perinatal lethality in mice","volume":"25","author":"Bader","year":"2005","journal-title":"Mol. Cell. Biol."},{"key":"2021042513255604900_DEV168948C8","doi-asserted-by":"publisher","first-page":"731","DOI":"10.1534\/genetics.111.126995","article-title":"The Drosophila gene disruption project: progress using transposons with distinctive site specificities","volume":"188","author":"Bellen","year":"2011","journal-title":"Genetics"},{"key":"2021042513255604900_DEV168948C9","doi-asserted-by":"publisher","first-page":"370","DOI":"10.1038\/46463","article-title":"A universal marker for transgenic insects","volume":"402","author":"Berghammer","year":"1999","journal-title":"Nature"},{"key":"2021042513255604900_DEV168948C10","doi-asserted-by":"publisher","first-page":"627","DOI":"10.1242\/dev.085100","article-title":"Cbl-associated protein regulates assembly and function of two tension-sensing structures in Drosophila","volume":"140","author":"Bharadwaj","year":"2013","journal-title":"Development"},{"key":"2021042513255604900_DEV168948C11","doi-asserted-by":"publisher","first-page":"468","DOI":"10.1007\/BF00377228","article-title":"The function of type IV collagen during Drosophila embryogenesis","volume":"205","author":"Borchiellini","year":"1996","journal-title":"Roux. Arch. Dev. Biol."},{"key":"2021042513255604900_DEV168948C12","doi-asserted-by":"publisher","first-page":"39620","DOI":"10.1074\/jbc.M607886200","article-title":"Loss of nidogen-1 and \u22122 results in syndactyly and changes in limb development","volume":"281","author":"B\u00f6se","year":"2006","journal-title":"J. Biol. Chem."},{"key":"2021042513255604900_DEV168948C13","doi-asserted-by":"publisher","first-page":"e1002531","DOI":"10.1371\/journal.pgen.1002531","article-title":"A machine learning approach for identifying novel cell type-specific transcriptional regulators of myogenesis","volume":"8","author":"Busser","year":"2012","journal-title":"PLoS Genet."},{"key":"2021042513255604900_DEV168948C14","doi-asserted-by":"publisher","first-page":"e69385","DOI":"10.1371\/journal.pone.0069385","article-title":"Contribution of distinct homeodomain DNA binding specificities to Drosophila embryonic mesodermal cell-specific gene expression programs","volume":"8","author":"Busser","year":"2013","journal-title":"PLoS ONE"},{"key":"2021042513255604900_DEV168948C15","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-662-22489-2","volume-title":"The Embryonic Development of Drosophila Melanogaster","author":"Campos-Ortega","year":"1997","edition":"2nd edn"},{"key":"2021042513255604900_DEV168948C16","doi-asserted-by":"crossref","first-page":"5209","DOI":"10.1016\/S0021-9258(19)69388-9","article-title":"Entactin, a novel basal lamina-associated sulfated glycoprotein","volume":"256","author":"Carlin","year":"1981","journal-title":"J. Biol. Chem."},{"key":"2021042513255604900_DEV168948C17","doi-asserted-by":"crossref","first-page":"3581","DOI":"10.1242\/dev.120.12.3581","article-title":"The novel homeodomain gene buttonless specifies differentiation and axonal guidance functions of Drosophila dorsal median cells","volume":"120","author":"Chiang","year":"1994","journal-title":"Development"},{"key":"2021042513255604900_DEV168948C18","doi-asserted-by":"publisher","first-page":"156","DOI":"10.1016\/0003-2697(87)90021-2","article-title":"Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction","volume":"162","author":"Chomczynski","year":"1987","journal-title":"Anal. Biochem."},{"key":"2021042513255604900_DEV168948C19","doi-asserted-by":"publisher","first-page":"337","DOI":"10.1016\/bs.ctm.2015.08.001","article-title":"Basement Membranes in the Worm: A Dynamic Scaffolding that Instructs Cellular Behaviors and Shapes Tissues","volume":"76","author":"Clay","year":"2015","journal-title":"Curr. Top. Membr."},{"key":"2021042513255604900_DEV168948C20","doi-asserted-by":"crossref","DOI":"10.1007\/978-1-59745-583-1","volume-title":"Drosophila: Methods and Protocols","author":"Dahmann","year":"2008"},{"key":"2021042513255604900_DEV168948C21","doi-asserted-by":"publisher","first-page":"e1007483","DOI":"10.1371\/journal.pgen.1007483","article-title":"Dissection of Nidogen function in Drosophila reveals tissue-specific mechanisms of basement membrane assembly","volume":"14","author":"Dai","year":"2018","journal-title":"PLoS Genet."},{"key":"2021042513255604900_DEV168948C22","doi-asserted-by":"crossref","first-page":"523","DOI":"10.1093\/genetics\/130.3.523","article-title":"l(1)trol and l(1)devl, loci affecting the development of the adult central nervous system in Drosophila melanogaster","volume":"130","author":"Datta","year":"1992","journal-title":"Genetics"},{"key":"2021042513255604900_DEV168948C23","doi-asserted-by":"publisher","first-page":"1617","DOI":"10.1097\/01.LAB.0000042240.52093.0F","article-title":"Neurologic defects and selective disruption of basement membranes in mice lacking entactin-1\/nidogen-1","volume":"82","author":"Dong","year":"2002","journal-title":"Lab. Invest."},{"key":"2021042513255604900_DEV168948C24","doi-asserted-by":"publisher","first-page":"5198","DOI":"10.1021\/bi00414a038","article-title":"Primary structure of the mouse laminin B2 chain and comparison with laminin B1","volume":"27","author":"Durkin","year":"1988","journal-title":"Biochemistry"},{"key":"2021042513255604900_DEV168948C25","doi-asserted-by":"publisher","first-page":"3897","DOI":"10.1242\/jeb.060533","article-title":"Associative learning between odorants and mechanosensory punishment in larval Drosophila","volume":"214","author":"Eschbach","year":"2011","journal-title":"J. Exp. Biol."},{"key":"2021042513255604900_DEV168948C26","doi-asserted-by":"publisher","first-page":"3137","DOI":"10.1002\/j.1460-2075.1991.tb04875.x","article-title":"Recombinant nidogen consists of three globular domains and mediates binding of laminin to collagen type IV","volume":"10","author":"Fox","year":"1991","journal-title":"EMBO J."},{"key":"2021042513255604900_DEV168948C27","doi-asserted-by":"publisher","first-page":"24","DOI":"10.1186\/1749-8104-3-24","article-title":"A synaptic nidogen: developmental regulation and role of nidogen-2 at the neuromuscular junction","volume":"3","author":"Fox","year":"2008","journal-title":"Neural Dev."},{"key":"2021042513255604900_DEV168948C28","doi-asserted-by":"publisher","first-page":"3149","DOI":"10.1046\/j.1432-1327.2000.01337.x","article-title":"Perlecan domain V of Drosophila melanogaster. Sequence, recombinant analysis and tissue expression","volume":"267","author":"Friedrich","year":"2000","journal-title":"Eur. J. Biochem."},{"key":"2021042513255604900_DEV168948C29","doi-asserted-by":"crossref","first-page":"2143","DOI":"10.1242\/dev.120.8.2143","article-title":"The role of tinman, a mesodermal cell fate gene, in axon pathfinding during the development of the transverse nerve in Drosophila","volume":"120","author":"Gorczyca","year":"1994","journal-title":"Development"},{"key":"2021042513255604900_DEV168948C30","doi-asserted-by":"publisher","first-page":"116","DOI":"10.1007\/BF00328463","article-title":"A preliminary genetic analysis of TE146, a very large transposing element of Drosophila melanogaster","volume":"92","author":"Gubb","year":"1985","journal-title":"Chromosoma"},{"key":"2021042513255604900_DEV168948C31","doi-asserted-by":"publisher","first-page":"6029","DOI":"10.1523\/JNEUROSCI.22-14-06029.2002","article-title":"A critical function of the pial basement membrane in cortical histogenesis","volume":"22","author":"Halfter","year":"2002","journal-title":"J. Neurosci."},{"key":"2021042513255604900_DEV168948C32","doi-asserted-by":"publisher","first-page":"63","DOI":"10.1016\/j.ymeth.2011.09.017","article-title":"A novel method for obtaining semi-thin cross sections of the Drosophila heart and their labeling with multiple antibodies","volume":"56","author":"Harpaz","year":"2012","journal-title":"Methods"},{"key":"2021042513255604900_DEV168948C33","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1038\/nature13996","article-title":"Spatiotemporal transcriptomics reveals the evolutionary history of the endoderm germ layer","volume":"519","author":"Hashimshony","year":"2015","journal-title":"Nature"},{"key":"2021042513255604900_DEV168948C34","doi-asserted-by":"crossref","first-page":"325","DOI":"10.1242\/dev.118.2.325","article-title":"Genetic analysis of laminin A reveals diverse functions during morphogenesis in Drosophila","volume":"118","author":"Henchcliffe","year":"1993","journal-title":"Development"},{"key":"2021042513255604900_DEV168948C35","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1002\/jemt.20567","article-title":"Nidogens-Extracellular matrix linker molecules","volume":"71","author":"Ho","year":"2008","journal-title":"Microsc. Res. Tech."},{"key":"2021042513255604900_DEV168948C36","doi-asserted-by":"publisher","first-page":"70","DOI":"10.1016\/S0959-4388(03)00002-3","article-title":"Development and maintenance of neuronal architecture at the ventral midline of C. elegans","volume":"13","author":"Hobert","year":"2003","journal-title":"Curr. Opin. Neurobiol."},{"key":"2021042513255604900_DEV168948C37","doi-asserted-by":"publisher","first-page":"56","DOI":"10.4161\/cam.21831","article-title":"Laminins in basement membrane assembly","volume":"7","author":"Hohenester","year":"2013","journal-title":"Cell Adh. Migr."},{"key":"2021042513255604900_DEV168948C38","doi-asserted-by":"publisher","first-page":"529","DOI":"10.1006\/jmbi.2001.4878","article-title":"Mapping of binding sites for nidogens, fibulin-2, fibronectin and heparin to different IG modules of perlecan","volume":"311","author":"Hopf","year":"2001","journal-title":"J. Mol. Biol."},{"key":"2021042513255604900_DEV168948C39","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1101\/gr.185579.114","article-title":"The Release 6 reference sequence of the Drosophila melanogaster genome","volume":"25","author":"Hoskins","year":"2015","journal-title":"Genome Res."},{"key":"2021042513255604900_DEV168948C40","doi-asserted-by":"publisher","first-page":"D245","DOI":"10.1093\/nar\/gkm977","article-title":"The 20 years of PROSITE","volume":"36","author":"Hulo","year":"2008","journal-title":"Nucleic Acids Res."},{"key":"2021042513255604900_DEV168948C41","doi-asserted-by":"publisher","first-page":"671","DOI":"10.1083\/jcb.201109041","article-title":"The evolution of metazoan extracellular matrix","volume":"196","author":"Hynes","year":"2012","journal-title":"J. Cell Biol."},{"key":"2021042513255604900_DEV168948C42","doi-asserted-by":"publisher","first-page":"2700","DOI":"10.1073\/pnas.79.8.2700","article-title":"Antibodies to horseradish peroxidase as specific neuronal markers in Drosophila and in grasshopper embryos","volume":"79","author":"Jan","year":"1982","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042513255604900_DEV168948C43","doi-asserted-by":"publisher","first-page":"R207","DOI":"10.1016\/j.cub.2017.02.006","article-title":"Basement membranes","volume":"27","author":"Jayadev","year":"2017","journal-title":"Curr. Biol."},{"key":"2021042513255604900_DEV168948C44","doi-asserted-by":"publisher","first-page":"3911","DOI":"10.1091\/mbc.11.11.3911","article-title":"Nidogen is nonessential and not required for normal type IV collagen localization in Caenorhabditis elegans","volume":"11","author":"Kang","year":"2000","journal-title":"Mol. Biol. Cell"},{"key":"2021042513255604900_DEV168948C45","doi-asserted-by":"publisher","first-page":"301","DOI":"10.1002\/cm.20124","article-title":"Jupiter, a new Drosophila protein associated with microtubules","volume":"63","author":"Karpova","year":"2006","journal-title":"Cell Motil. Cytoskelet."},{"key":"2021042513255604900_DEV168948C46","doi-asserted-by":"publisher","first-page":"150","DOI":"10.1126\/science.288.5463.150","article-title":"Positioning of longitudinal nerves in C. elegans by nidogen","volume":"288","author":"Kim","year":"2000","journal-title":"Science"},{"key":"2021042513255604900_DEV168948C47","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1895\/wormbook.1.16.1","article-title":"Basement membranes","author":"Kramer","year":"2005","journal-title":"WormBook"},{"key":"2021042513255604900_DEV168948C48","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1007\/978-1-59745-583-1_18","article-title":"Fluorescent in situ hybridization protocols in Drosophila embryos and tissues","volume":"420","author":"L\u00e9cuyer","year":"2008","journal-title":"Methods Mol. Biol."},{"key":"2021042513255604900_DEV168948C49","doi-asserted-by":"publisher","first-page":"217","DOI":"10.1111\/j.1768-322X.1986.tb00453.x","article-title":"Characterization and expression of collagen-like genes in Drosophila melanogaster","volume":"56","author":"Le Parco","year":"1986","journal-title":"Biol. Cell"},{"key":"2021042513255604900_DEV168948C50","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/B978-0-12-450990-0.50005-8","article-title":"GENES","volume-title":"The Genome of Drosophila Melanogaster","author":"Lindsley","year":"1992"},{"key":"2021042513255604900_DEV168948C51","doi-asserted-by":"crossref","first-page":"18318","DOI":"10.1016\/S0021-9258(19)81362-5","article-title":"Drosophila basement membrane procollagen IV. I. Protein characterization and distribution","volume":"263","author":"Lunstrum","year":"1988","journal-title":"J. Biol. Chem."},{"key":"2021042513255604900_DEV168948C52","volume-title":"Molecular Cloning: a Laboratory Manual\/T. Maniatis, E.F. Fritsch, J. Sambrook","author":"Maniatis","year":"1982"},{"key":"2021042513255604900_DEV168948C53","doi-asserted-by":"publisher","first-page":"65","DOI":"10.1002\/j.1460-2075.1989.tb03349.x","article-title":"Amino acid sequence of mouse nidogen, a multidomain basement membrane protein with binding activity for laminin, collagen IV and cells","volume":"8","author":"Mann","year":"1989","journal-title":"EMBO J."},{"key":"2021042513255604900_DEV168948C54","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1146\/annurev.cb.03.110187.000421","article-title":"Laminin and other basement membrane components","volume":"3","author":"Martin","year":"1987","journal-title":"Annu. Rev. Cell Biol."},{"key":"2021042513255604900_DEV168948C55","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1083\/jcb.145.1.191","article-title":"wing blister, a new Drosophila laminin alpha chain required for cell adhesion and migration during embryonic and imaginal development","volume":"145","author":"Martin","year":"1999","journal-title":"J. Cell Biol."},{"key":"2021042513255604900_DEV168948C56","doi-asserted-by":"publisher","first-page":"3526","DOI":"10.1016\/j.cub.2017.10.001","article-title":"A moving source of matrix components is essential for de novo basement membrane formation","volume":"27","author":"Matsubayashi","year":"2017","journal-title":"Curr. Biol."},{"key":"2021042513255604900_DEV168948C57","doi-asserted-by":"publisher","first-page":"1879","DOI":"10.1002\/j.1460-2075.1993.tb05836.x","article-title":"A single EGF-like motif of laminin is responsible for high affinity nidogen binding","volume":"12","author":"Mayer","year":"1993","journal-title":"EMBO J."},{"key":"2021042513255604900_DEV168948C58","doi-asserted-by":"publisher","first-page":"681","DOI":"10.1111\/j.1432-1033.1995.tb20188.x","article-title":"Binding properties and protease stability of recombinant human nidogen","volume":"227","author":"Mayer","year":"1995","journal-title":"Eur. J. Biochem."},{"key":"2021042513255604900_DEV168948C59","doi-asserted-by":"publisher","first-page":"571","DOI":"10.1534\/genetics.105.041848","article-title":"Minos as a genetic and genomic tool in Drosophila melanogaster","volume":"171","author":"Metaxakis","year":"2005","journal-title":"Genetics"},{"key":"2021042513255604900_DEV168948C60","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1111\/j.1440-169X.2009.01111.x","article-title":"Drosophila multiplexin (Dmp) modulates motor axon pathfinding accuracy","volume":"51","author":"Meyer","year":"2009","journal-title":"Dev. Growth Differ."},{"key":"2021042513255604900_DEV168948C61","doi-asserted-by":"publisher","first-page":"146","DOI":"10.1002\/jnr.490310120","article-title":"Collagen IV is present in the developing CNS during Drosophila neurogenesis","volume":"31","author":"Mirre","year":"1992","journal-title":"J. Neurosci. Res."},{"key":"2021042513255604900_DEV168948C62","doi-asserted-by":"publisher","first-page":"2441","DOI":"10.1083\/jcb.109.5.2441","article-title":"Drosophila laminin: sequence of B2 subunit and expression of all three subunits during embryogenesis","volume":"109","author":"Montell","year":"1989","journal-title":"J. Cell Biol."},{"key":"2021042513255604900_DEV168948C63","doi-asserted-by":"publisher","first-page":"7007","DOI":"10.1128\/MCB.20.18.7007-7012.2000","article-title":"The absence of nidogen 1 does not affect murine basement membrane formation","volume":"20","author":"Murshed","year":"2000","journal-title":"Mol. Cell. Biol."},{"key":"2021042513255604900_DEV168948C64","doi-asserted-by":"publisher","first-page":"11","DOI":"10.1111\/j.1432-1033.1993.tb17729.x","article-title":"Ascidian entactin\/nidogen. Implication of evolution by shuffling two kinds of cysteine-rich motifs","volume":"213","author":"Nakae","year":"1993","journal-title":"Eur. J. Biochem."},{"key":"2021042513255604900_DEV168948C65","doi-asserted-by":"crossref","first-page":"621","DOI":"10.1093\/genetics\/120.3.621","article-title":"Genetic applications of an inverse polymerase chain reaction","volume":"120","author":"Ochman","year":"1988","journal-title":"Genetics"},{"key":"2021042513255604900_DEV168948C66","doi-asserted-by":"publisher","first-page":"e71706","DOI":"10.1371\/journal.pone.0071706","article-title":"High-throughput analysis of stimulus-evoked behaviors in Drosophila larva reveals multiple modality-specific escape strategies","volume":"8","author":"Ohyama","year":"2013","journal-title":"PLoS ONE"},{"key":"2021042513255604900_DEV168948C67","doi-asserted-by":"publisher","first-page":"1219","DOI":"10.1002\/j.1460-2075.1990.tb08229.x","article-title":"Glutactin, a novel Drosophila basement membrane-related glycoprotein with sequence similarity to serine esterases","volume":"9","author":"Olson","year":"1990","journal-title":"EMBO J."},{"key":"2021042513255604900_DEV168948C68","doi-asserted-by":"publisher","first-page":"245","DOI":"10.1016\/j.devcel.2011.06.026","article-title":"Shaping cells and organs in Drosophila by opposing roles of fat body-secreted Collagen IV and perlecan","volume":"21","author":"Pastor-Pareja","year":"2011","journal-title":"Dev. Cell"},{"key":"2021042513255604900_DEV168948C69","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1007\/s00427-012-0402-z","article-title":"Activation of Hox genes during caudal regeneration of the polychaete annelid Platynereis dumerilii","volume":"222","author":"Pfeifer","year":"2012","journal-title":"Dev. Genes Evol."},{"key":"2021042513255604900_DEV168948C70","doi-asserted-by":"publisher","first-page":"e53","DOI":"10.1371\/journal.pcbi.0020053","article-title":"Expression-guided in silico evaluation of candidate cis regulatory codes for Drosophila muscle founder cells","volume":"2","author":"Philippakis","year":"2006","journal-title":"PLoS Comput. Biol."},{"key":"2021042513255604900_DEV168948C71","doi-asserted-by":"publisher","first-page":"1619","DOI":"10.1242\/dev.01037","article-title":"Collagen IV is essential for basement membrane stability but dispensable for initiation of its assembly during early development","volume":"131","author":"Poschl","year":"2004","journal-title":"Development"},{"key":"2021042513255604900_DEV168948C72","doi-asserted-by":"publisher","first-page":"2281","DOI":"10.1093\/bioinformatics\/btp381","article-title":"FancyGene: dynamic visualization of gene structures and protein domain architectures on genomic loci","volume":"25","author":"Rambaldi","year":"2009","journal-title":"Bioinformatics"},{"key":"2021042513255604900_DEV168948C73","doi-asserted-by":"crossref","first-page":"10881","DOI":"10.1016\/S0021-9258(18)82067-1","article-title":"Mapping of nidogen binding sites for collagen type IV, heparan sulfate proteoglycan, and zinc","volume":"268","author":"Reinhardt","year":"1993","journal-title":"J. Biol. Chem."},{"key":"2021042513255604900_DEV168948C74","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1093\/genetics\/143.2.929","article-title":"Identification of immune system and response genes, and novel mutations causing melanotic tumor formation in Drosophila melanogaster","volume":"143","author":"Rodriguez","year":"1996","journal-title":"Genetics"},{"key":"2021042513255604900_DEV168948C75","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1038\/nature14580","article-title":"Genetic compensation induced by deleterious mutations but not gene knockdowns","volume":"524","author":"Rossi","year":"2015","journal-title":"Nature"},{"key":"2021042513255604900_DEV168948C76","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","article-title":"Fiji: an open-source platform for biological-image analysis","volume":"9","author":"Schindelin","year":"2012","journal-title":"Nat. Methods"},{"key":"2021042513255604900_DEV168948C77","doi-asserted-by":"publisher","first-page":"6820","DOI":"10.1128\/MCB.22.19.6820-6830.2002","article-title":"Gene structure and functional analysis of the mouse nidogen-2 gene: nidogen-2 is not essential for basement membrane formation in mice","volume":"22","author":"Schymeinsky","year":"2002","journal-title":"Mol. Cell. Biol."},{"key":"2021042513255604900_DEV168948C78","doi-asserted-by":"publisher","first-page":"18074","DOI":"10.1074\/jbc.271.30.18074","article-title":"Conserved neuron promoting activity in Drosophila and vertebrate laminin alpha1","volume":"271","author":"Takagi","year":"1996","journal-title":"J. Biol. Chem."},{"key":"2021042513255604900_DEV168948C79","doi-asserted-by":"publisher","first-page":"1413","DOI":"10.1038\/ncb1660","article-title":"Integrin-dependent anchoring of a stem-cell niche","volume":"9","author":"Tanentzapf","year":"2007","journal-title":"Nat. Cell Biol."},{"key":"2021042513255604900_DEV168948C80","doi-asserted-by":"publisher","first-page":"123","DOI":"10.1002\/bies.950180208","article-title":"Supramolecular assembly of basement membranes","volume":"18","author":"Timpl","year":"1996","journal-title":"BioEssays"},{"key":"2021042513255604900_DEV168948C81","doi-asserted-by":"publisher","first-page":"455","DOI":"10.1111\/j.1432-1033.1983.tb07849.x","article-title":"Nidogen: a new, self-aggregating basement membrane protein","volume":"137","author":"Timpl","year":"1983","journal-title":"Eur. J. Biochem."},{"key":"2021042513255604900_DEV168948C82","doi-asserted-by":"publisher","first-page":"17699","DOI":"10.1073\/pnas.1206416109","article-title":"Activity-dependent retrograde laminin A signaling regulates synapse growth at Drosophila neuromuscular junctions","volume":"109","author":"Tsai","year":"2012","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"2021042513255604900_DEV168948C83","doi-asserted-by":"publisher","first-page":"3693","DOI":"10.1021\/pr5002065","article-title":"Proteomic view of basement membranes from human retinal blood vessels, inner limiting membranes, and lens capsules","volume":"13","author":"Uechi","year":"2014","journal-title":"J. Proteome Res."},{"key":"2021042513255604900_DEV168948C84","doi-asserted-by":"publisher","first-page":"4165","DOI":"10.1242\/dev.044263","article-title":"Drosophila laminins act as key regulators of basement membrane assembly and morphogenesis","volume":"136","author":"Urbano","year":"2009","journal-title":"Development"},{"key":"2021042513255604900_DEV168948C85","doi-asserted-by":"publisher","first-page":"403","DOI":"10.1002\/dvdy.10120","article-title":"Perlecan participates in proliferation activation of quiescent Drosophila neuroblasts","volume":"224","author":"Voigt","year":"2002","journal-title":"Dev. Dyn."},{"key":"2021042513255604900_DEV168948C86","doi-asserted-by":"publisher","first-page":"833","DOI":"10.1016\/j.neuron.2006.02.008","article-title":"Bruchpilot, a protein with homology to ELKS\/CAST, is required for structural integrity and function of synaptic active zones in Drosophila","volume":"49","author":"Wagh","year":"2006","journal-title":"Neuron"},{"key":"2021042513255604900_DEV168948C87","doi-asserted-by":"crossref","first-page":"2711","DOI":"10.1242\/dev.129.11.2711","article-title":"Specific ablation of the nidogen-binding site in the laminin gamma1 chain interferes with kidney and lung development","volume":"129","author":"Willem","year":"2002","journal-title":"Development"},{"key":"2021042513255604900_DEV168948C88","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1007\/s00018-011-0652-3","article-title":"The role of LamininB2 (LanB2) during mesoderm differentiation in Drosophila","volume":"69","author":"Wolfstetter","year":"2012","journal-title":"Cell. Mol. Life Sci."},{"key":"2021042513255604900_DEV168948C89","doi-asserted-by":"publisher","first-page":"721","DOI":"10.1016\/j.mod.2009.05.001","article-title":"Fusion of circular and longitudinal muscles in Drosophila is independent of the endoderm but further visceral muscle differentiation requires a close contact between mesoderm and endoderm","volume":"126","author":"Wolfstetter","year":"2009","journal-title":"Mech. Dev."},{"key":"2021042513255604900_DEV168948C90","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1016\/j.neuron.2011.02.050","article-title":"A combinatorial semaphorin code instructs the initial steps of sensory circuit assembly in the Drosophila CNS","volume":"70","author":"Wu","year":"2011","journal-title":"Neuron"},{"key":"2021042513255604900_DEV168948C91","doi-asserted-by":"publisher","first-page":"1095","DOI":"10.1007\/s00018-016-2381-0","article-title":"Laminin: loss-of-function studies","volume":"74","author":"Yao","year":"2017","journal-title":"Cell. Mol. Life Sci."},{"key":"2021042513255604900_DEV168948C92","doi-asserted-by":"publisher","first-page":"609","DOI":"10.1006\/dbio.1995.1173","article-title":"Laminin is required for heart, somatic muscles, and gut development in the Drosophila embryo","volume":"169","author":"Yarnitzky","year":"1995","journal-title":"Dev. Biol."},{"key":"2021042513255604900_DEV168948C93","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1016\/S0378-1119(97)00274-6","article-title":"viking: identification and characterization of a second type IV collagen in Drosophila","volume":"198","author":"Yasothornsrikul","year":"1997","journal-title":"Gene"},{"key":"2021042513255604900_DEV168948C94","doi-asserted-by":"publisher","first-page":"a004911","DOI":"10.1101\/cshperspect.a004911","article-title":"Basement membranes: cell scaffoldings and signaling platforms","volume":"3","author":"Yurchenco","year":"2011","journal-title":"Cold Spring Harb Perspect Biol"},{"key":"2021042513255604900_DEV168948C95","doi-asserted-by":"publisher","first-page":"1457","DOI":"10.1242\/dev.069005","article-title":"Differential regulation of mesodermal gene expression by Drosophila cell type-specific Forkhead transcription factors","volume":"139","author":"Zhu","year":"2012","journal-title":"Development"},{"key":"2021042513255604900_DEV168948C96","doi-asserted-by":"publisher","first-page":"553","DOI":"10.1016\/j.jgg.2017.09.011","article-title":"Short body length phenotype is compensated by the upregulation of nidogen family members in a deleterious nid1a mutation of zebrafish","volume":"44","author":"Zhu","year":"2017","journal-title":"J. Genet. Genomics"}],"container-title":["Development"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.1242\/dev.168948","content-type":"unspecified","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/doi\/10.1242\/dev.168948\/1871068\/dev168948.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/doi\/10.1242\/dev.168948\/2055201\/dev_168948v1.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"http:\/\/journals.biologists.com\/dev\/article-pdf\/doi\/10.1242\/dev.168948\/2055201\/dev_168948v1.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,12]],"date-time":"2023-09-12T05:02:43Z","timestamp":1694494963000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.biologists.com\/dev\/article\/doi\/10.1242\/dev.168948\/264783\/Characterization-of-Drosophila-Nidogen-entactin"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1,1]]},"references-count":96,"URL":"https:\/\/doi.org\/10.1242\/dev.168948","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/348631","asserted-by":"object"}]},"ISSN":["1477-9129","0950-1991"],"issn-type":[{"value":"1477-9129","type":"electronic"},{"value":"0950-1991","type":"print"}],"subject":[],"published":{"date-parts":[[2018,1,1]]},"article-number":"dev.168948"}}