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Here, we show that laminin alpha 2 (lm-\u03b12) is a component of the midbrain dopaminergic (mDA) progenitor niche in the ventral midbrain (VM) and identify a concentration-dependent role for lm211 in regulating mDA progenitor proliferation and survival via distinct set of receptors. At high-concentrations, lm211 rich environments maintain mDA progenitors in a proliferative state via integrins \u03b16\u03b21 and \u03b17\u03b21. Whereas low concentrations of lm211 support mDA lineage survival via dystroglycan receptors. We confirmed our findings in vivo where, in the absence of lm-\u03b12, the VM was smaller, with increased apoptosis, and the progenitor pool depleted through premature differentiation resulting in fewer mDA neurons. In examining mDA neuron subtype composition we found a reduction in later-born mDA neurons of the ventral tegmental area, which control a range of cognitive behaviours. Our results identify a novel role for lm in neural development and provide a possible mechanism for autism-like behaviours and brainstem hypoplasia seen in some patients with mutations of the human lm-\u03b12 gene.<\/jats:p>","DOI":"10.1242\/dev.172668","type":"journal-article","created":{"date-parts":[[2019,8,1]],"date-time":"2019-08-01T21:09:11Z","timestamp":1564693751000},"source":"Crossref","is-referenced-by-count":16,"title":["Laminin \u03b12 controls mouse and human stem cell behaviour during midbrain dopaminergic neuron development"],"prefix":"10.1242","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8539-7462","authenticated-orcid":false,"given":"Maqsood","family":"Ahmed","sequence":"first","affiliation":[{"name":"MRC Centre of Regenerative Medicine, University of Edinburgh, EH16 4UU, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leandro N.","family":"Marziali","sequence":"additional","affiliation":[{"name":"Departments of Biochemistry and Neurology, School of Medicine and Biomedical Sciences, University at Buffalo, Buffalo, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernest","family":"Arenas","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Neurobiology, Department of Medical Biochemistry and Biophysics, Karolinska Institutet, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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