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Although molecular signals have been implicated in this process and linked to congenital abnormalities, few studies have examined the role of mechanical forces. In this study, we quantified morphology, cell proliferation and tissue growth in the forebrain of chicken embryos during Hamburger-Hamilton stages 17-21. By altering embryonic cerebrospinal fluid pressure during development, we found that neuroepithelial growth depends on not only chemical morphogen gradients but also mechanical feedback. Using these data, as well as published information on morphogen activity, we developed a chemomechanical growth law to mathematically describe growth of the neuroepithelium. Finally, we constructed a three-dimensional computational model based on these laws, with all parameters based on experimental data. The resulting model predicts forebrain shapes consistent with observations in normal embryos, as well as observations under chemical or mechanical perturbation. These results suggest that molecular and mechanical signals play important roles in early forebrain morphogenesis and may contribute to the development of congenital malformations.<\/jats:p>","DOI":"10.1242\/dev.174318","type":"journal-article","created":{"date-parts":[[2019,10,11]],"date-time":"2019-10-11T17:25:23Z","timestamp":1570814723000},"source":"Crossref","is-referenced-by-count":40,"title":["Molecular and mechanical signals determine morphogenesis of the cerebral hemispheres in the chicken embryo"],"prefix":"10.1242","volume":"146","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1325-0749","authenticated-orcid":false,"given":"Kara E.","family":"Garcia","sequence":"first","affiliation":[{"name":"Department of Biomedical Engineering, Washington University, 1 Brookings Drive, St. Louis, MO 63130, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wade G.","family":"Stewart","sequence":"additional","affiliation":[{"name":"Department of Biomedical Engineering, University of Delaware, 150 Academy Street, Newark, DE 19716, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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