{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T19:12:05Z","timestamp":1790536325512,"version":"4.1.0"},"reference-count":85,"publisher":"The Company of Biologists","license":[{"start":{"date-parts":[[2019,1,1]],"date-time":"2019-01-01T00:00:00Z","timestamp":1546300800000},"content-version":"am","delay-in-days":0,"URL":"http:\/\/www.biologists.com\/user-licence-1-1\/"}],"funder":[{"DOI":"10.13039\/501100009053","name":"DBT India Alliance","doi-asserted-by":"publisher","award":["IA\/I\/15\/2\/502077"],"award-info":[{"award-number":["IA\/I\/15\/2\/502077"]}],"id":[{"id":"10.13039\/501100009053","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:p>Proper establishment of cell polarity is essential for development. In the one-cell C. elegans embryo, a centrosome-localised signal provides spatial information for polarity establishment. It is hypothesised that this signal causes local inhibition of the cortical actomyosin network, and breaks symmetry to direct partitioning of the PAR proteins. However, the molecular nature of the centrosomal signal that triggers cortical anisotropy in the actomyosin network to promote polarity establishment remains elusive. Here, we discover that depletion of Aurora A kinase (AIR-1 in C. elegans) causes pronounced cortical contractions on the embryo surface, and this creates more than one PAR-2 polarity axis. This function of AIR-1 appears independent of its role in microtubule nucleation. Importantly, upon AIR-1 depletion, centrosome positioning becomes dispensable in dictating the PAR-2 axis. Moreover, we uncovered that a Rho GEF, ECT-2 acts downstream to AIR-1 in regulating contractility and PAR-2 localisation, and notably, AIR-1 depletion influences ECT-2 cortical localisation. Overall, our study unravels a novel insight whereby an evolutionarily conserved centrosome Aurora A kinase inhibits promiscuous PAR-2 domain formation to ensure singularity in the polarity establishment axis.<\/jats:p>","DOI":"10.1242\/dev.174565","type":"journal-article","created":{"date-parts":[[2019,10,21]],"date-time":"2019-10-21T13:45:33Z","timestamp":1571665533000},"source":"Crossref","is-referenced-by-count":36,"title":["Centrosome Aurora A regulates RhoGEF ECT-2 localisation and ensures a single PAR-2 polarity axis in\n                    <i>C. elegans<\/i>\n                    embryos"],"prefix":"10.1242","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7391-5610","authenticated-orcid":false,"given":"Sukriti","family":"Kapoor","sequence":"first","affiliation":[{"name":"Department of Microbiology and Cell Biology (MCB), Indian Institute of Science (IISc), Bangalore, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0219-7970","authenticated-orcid":false,"given":"Sachin","family":"Kotak","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Cell Biology (MCB), Indian Institute of Science (IISc), Bangalore, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2019,1,1]]},"reference":[{"key":"2021042622580454200_DEV174565C1","doi-asserted-by":"publisher","first-page":"237","DOI":"10.1242\/dev.042754","article-title":"Regulation of cortical contractility and spindle positioning by the protein phosphatase 6 PPH-6 in one-cell stage C. elegans embryos","volume":"137","author":"Afshar","year":"2010","journal-title":"Development"},{"key":"2021042622580454200_DEV174565C2","doi-asserted-by":"publisher","first-page":"267","DOI":"10.1083\/jcb.200506124","article-title":"A complex containing the Sm protein CAR-1 and the RNA helicase CGH-1 is required for embryonic cytokinesis in Caenorhabditis elegans","volume":"171","author":"Audhya","year":"2005","journal-title":"J. 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