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In chronic airway diseases, defective repair leads to tissue remodeling. Delineating key drivers of differentiation dynamics can help understand how normal or pathological regeneration occurs. Using single-cell transcriptomics and lineage inference, we have unraveled trajectories from basal to luminal cells, providing novel markers for specific populations. We report that: (1) a precursor subgroup of multiciliated cells, which we have entitled deuterosomal cells, is defined by specific markers, such as DEUP1, FOXN4, YPEL1, HES6 and CDC20B; (2) goblet cells can be precursors of multiciliated cells, thus explaining the presence of hybrid cells that co-express markers of goblet and multiciliated cells; and (3) a repertoire of molecules involved in the regeneration process, such as keratins or components of the Notch, Wnt or BMP\/TGF\u03b2 pathways, can be identified. Confirmation of our results on fresh human and pig airway samples, and on mouse tracheal cells, extend and confirm our conclusions regarding the molecular and cellular choreography at work during mucociliary epithelial differentiation.<\/jats:p>","DOI":"10.1242\/dev.177428","type":"journal-article","created":{"date-parts":[[2019,9,26]],"date-time":"2019-09-26T16:12:48Z","timestamp":1569514368000},"source":"Crossref","is-referenced-by-count":314,"title":["Novel dynamics of human mucociliary differentiation revealed by single-cell RNA sequencing of nasal epithelial cultures"],"prefix":"10.1242","volume":"146","author":[{"given":"Sandra","family":"Ruiz Garc\u00eda","sequence":"first","affiliation":[{"name":"Universit\u00e9 C\u00f4te d'Azur, CNRS, IPMC, Sophia-Antipolis 06560, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Deprez","sequence":"additional","affiliation":[{"name":"Universit\u00e9 C\u00f4te d'Azur, CNRS, IPMC, Sophia-Antipolis 06560, 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