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Cellular morphogenesis of sperm occurs during the post-meiotic developmental stage; however, little is known about what coordinates this process. In the present study, we investigated the role of A-kinase anchoring protein 3 (AKAP3) during mouse spermiogenesis, using both mouse genetics and proteomics. It was found that AKAP3 is essential for the formation of the specific subcellular structure of the sperm flagellum, motility of sperm and male fertility. Additionally, lack of AKAP3 caused global changes of the sperm proteome and mislocalization of sperm proteins, including accumulation of RNA metabolism and translation factors and displacement of PKA subunits in mature sperm, which may underlie misregulated PKA activity and immotility in sperm. Interestingly, sperm lacking a complete fibrous sheath from both Akap3 and Akap4 null mice accumulated F-actin filaments and morphological defects during post-testicular maturation in the epididymis. These results suggest that the subcellular structures of sperm could be formed via independent pathways, and elucidate the roles of AKAP3 during the coordinated synthesis and organization of the sperm proteome and sperm morphology.<\/jats:p>","DOI":"10.1242\/dev.181057","type":"journal-article","created":{"date-parts":[[2020,1,22]],"date-time":"2020-01-22T15:25:15Z","timestamp":1579706715000},"source":"Crossref","is-referenced-by-count":86,"title":["Lack of AKAP3 disrupts integrity of the subcellular structure and proteome of mouse sperm and causes male sterility"],"prefix":"10.1242","volume":"147","author":[{"given":"Kaibiao","family":"Xu","sequence":"first","affiliation":[{"name":"South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences 1 CAS Key Laboratory of Regenerative Biology , , Guangzhou 510630 , China"},{"name":"South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences 2 Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine , , Guangzhou 510630 , China"},{"name":"University of Chinese Academy of Sciences 3 , Beijing 100049 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lele","family":"Yang","sequence":"additional","affiliation":[{"name":"South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences 1 CAS Key Laboratory of Regenerative Biology , , Guangzhou 510630 , China"},{"name":"South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences 2 Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine , , Guangzhou 510630 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Zhang","sequence":"additional","affiliation":[{"name":"Guangzhou Medical University 4 GIBH-GMU Joint-school of Biological Sciences , , Guangzhou 511436 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1539-5650","authenticated-orcid":false,"given":"Huayu","family":"Qi","sequence":"additional","affiliation":[{"name":"South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences 1 CAS Key Laboratory of Regenerative Biology , , Guangzhou 510630 , China"},{"name":"South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences 2 Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine , , Guangzhou 510630 , China"},{"name":"University of Chinese Academy of Sciences 3 , Beijing 100049 , China"},{"name":"Guangzhou Medical University 4 GIBH-GMU Joint-school of Biological Sciences , , Guangzhou 511436 , China"},{"name":"Guangzhou Regenerative Medicine and Health Guangdong Laboratory 5 , Guangzhou 510005 , China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"237","published-online":{"date-parts":[[2020,1,22]]},"reference":[{"key":"2024061301114241900_DEV181057C1","doi-asserted-by":"publisher","first-page":"2790","DOI":"10.1093\/humrep\/dei126","article-title":"Gene deletions in an infertile man with sperm fibrous sheath dysplasia","volume":"20","author":"Baccetti","year":"2005","journal-title":"Hum. 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